Retention behavior of multiple sulfonamides in various liquid chromatographic systems

Botsoglou, Nikolaos; Fletouris, Dimitrios; Simeonidou, E. J.; Psomas, Ioannis E.

doi:10.1007/bf02496364

Cited by 6 publications

(2 citation statements)

References 19 publications

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“…38 Additionally, very weakly basic or acidic sites may also be present in the side R-group, which can also play a crucial role in retention on the stationary phase. As observed by Botsoglou et al, 39 the retention time of the investigated sulfonamides increased slightly in the pH range 2.5-3.5, the retention being greatest for sulfonamides bearing an additional methyl group on the R-side-chain. This suggests that the R-side-chain plays a crucial role in the hydrophobic interactions of the compounds with the stationary phase; 39 this was also observed in the present experiments.…”

Section: Discussionsupporting

confidence: 73%

A very fast and simple method for the determination of sulfonamide residues in seawaters

et al. 2011

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The development of simple and reproducible analytical methods for the determination of new organic pollutants like pharmaceuticals and personal care products in seawaters is of the highest priority. In the present study, a new analytical method based on offline solid phase extraction and liquid chromatography with standard UV detection (SPE-HPLC-UV) was developed to determine the presence of sulfonamides in seawaters. Special attention was paid to the sample preparation step. Different variables affecting the extraction process, such as seawater salinity and the humic acid content were studied. As a result, the presented SPE procedure for the extraction of sulfonamides from seawaters is also applicable to other techniques like LC-MS and LC-MS/MS. This method was optimized and fully validated for its performance parameters. It has very good selectivity, linearity (R 2 > 0.995), precision (RSD < 5%), accuracy (76.7-115.4%), as well as low limits of detection (LOD, 167 ng L À1 ) and quantification (LOQ, 500 ng L À1 ). This SPE procedure proved to be very effective (absolute recovery >75%), even in highly saline waters and in the presence of humic acids.

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Section: Discussionsupporting

confidence: 73%

A very fast and simple method for the determination of sulfonamide residues in seawaters

et al. 2011

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“…Changes in the retention of a series of sulfonamides on a polystyrene-divinylbenzene (PS-DVB) column with the pH of the eluent are compared with separations in conventional eluents. These drugs have been of considerable analytical interest over many years and a large number of methods have been reported for their determination, including reversed-phase liquid chromatography with buffered eluents on both ODS silica [6][7][8][9][10] and PS-DVB columns [11][12][13] and more recently capillary electrophoresis [14][15][16]. Changes in retention over a limited pH range are used to determine if the apparent pKa of the analytes is changed significantly by the elevated temperature.…”

Section: Introductionmentioning

confidence: 99%

Buffered superheated water as an eluent for reversed-phase high performance liquid chromatography

Chienthavorn

Smith

1999

Chromatographia

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SummaryInorganic buffers have been used to control the pH of a superheated water eluent for reversed-phase liquid chromatography. Using a temperature gradient from 70-190 ~ the relative order of elution of a series of sulfonamides on a polystyrene-divinyl benzene column was determined at pH 3, 7 and 11. The separations were compared with conventional reversed-phase separations on polymer and ODS-bonded silica columns. The apparent pKa values of selected sulfonamides at elevated temperatures were determined from their retention factors across in a range of superheated buffers and the values were compared to those reported at room temperature.

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Antibiotics, Pharmaceutical Analysis of

Gilpin

Pachla³

2000

Encyclopedia of Analytical Chemistry

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An antibiotic is a chemical substance, produced by microorganisms (or recently, synthetic routes) which has the capacity to inhibit the growth of other microorganisms/bacterial agents. The terminology for antibiotics stems from the Latin term of antibiosis and the noun antibiotic was coined by Professor Waksman in 1942. Considered as a single therapeutic class, antibiotics are one of the most diverse groups of medicinal agents. They can be grouped into several subclasses depending on their source, structure and activity. These are the cephalosporins, penicillins, quinolones, streptomyces and tetracycline. In addition to these agents, the sulfonamides and other miscellaneous compounds are also included owing to their antibacterial activity.

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Retention behavior of multiple sulfonamides in various liquid chromatographic systems

Cited by 6 publications

References 19 publications

A very fast and simple method for the determination of sulfonamide residues in seawaters

A very fast and simple method for the determination of sulfonamide residues in seawaters

Buffered superheated water as an eluent for reversed-phase high performance liquid chromatography

Antibiotics, Pharmaceutical Analysis of

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