Retention Behavior of Various Aromatic Compounds on Poly(butylene terephthalate) Stationary Phase in Liquid Chromatography

Abstract

Poly(butylene terephthalate)-coated silica (PBT) have been introduced as a stationary phase in liquid chromatography (LC) and the retention behavior of polycyclic aromatic compounds (PACs) was evaluated in reversed-phase LC. The trend for the retention was compared with that obtained on two types of commercially-available octadecylisilica (ODS) phases and phenylbutylsilica (PBS) phase. A good liner relationship between molecular size of planar PACs and the corresponding logarithmic retention factor was confirmed on the PBT stationary phase, and the trend is quite similar to that obtained on a conventional polymeric ODS stationary phase. In addition, a good molecular shape recognition capability of the PBT stationary phase was confirmed for several solute pairs consisted of planar and non-planar PACs with a similar two-dimensional molecular size. The selectivities to some planar/non-planar solute pairs on the PBT stationary phase were significantly better than conventional ODS phases, even when compared with that of typical polymeric ODS stationary phases operated in a similar experimental condition. In the case of structural isomers of dichlorobenzene and dibromobenzene, the elution order on the PBT stationary phase was o-, m-and p-, however the corresponding elution order in typical ODS phases, and PBS phase was different, o-, p-and m-. The results can be explained on the basis of the molecular-molecular interaction between the stationary phase ligand and the analyte molecule, because the PBT stationary phase has a similar partial chemical structure to these p-isomers on the silica support.

Molecular Shape Selectivity for Polycyclic Aromatic Compoundson a Poly(4-vinylpyridine) Stationary Phase in Liquid Chromatography

“…The F number was proposed by Hurtubise et al [45,46] and defined as follows: F = (number of double bonds) + (number of primary and secondary carbons) -0.5 × (number of non-aromatic rings), and for planar PACs, a high linear correlation between the logarithmic retention factor and F number has been confirmed for typical monomeric and polymeric ODS stationary phases in aqueous LC conditions [47]. Regarding the relationship between the F number of PACs and the corresponding logarithmic retention factor, a similar trend was also observed on the PBS phase in our previous work [48]. As shown in Fig.…”

Fibrous Polyimide Stationary Phase for the Separation of Aromatic Compounds in Reversed-Phase Liquid Chromatography

“…However, the introduction of synthetic polymer materials as a support for the stationary phase can counter the several disadvantages of silicagel support [32]. One of the advantages of the polymer-based stationary phase is the easy synthesis of the particle as the support of stationary phase, and in addition, the designs of the chemical structure of the ligands are very flexible [33,34]. Various types of synthetic polymer materials have been developed; thus, many candidates are available for the support of separation media in separation science.…”

Molecular Shape Selectivity for Polycyclic Aromatic Compounds on a Poly(benzoguanamine-<i>co</i>-melamine-<i>co</i>-formaldehyde) Stationary Phase in Reversed-Phase Liquid Chromatography

“…Liquid chromatography (LC) is one of the most powerful and convenient separation methods, especially for the separation of non-volatile sample matrix in separation science [1][2][3][4]. In order to obtain an effective separation of the complex sample mixtures commonly encountered in actual separation problems, the stationary phase plays an essential role [5][6][7][8]. Development of novel stationary phases has been regarded as a key process to create more efficient and cost-effective separations.…”