Retention behaviour of amylopectins in asymmetrical flow field-flow fractionation studied by multi-angle light scattering detection

Bruijnsvoort, M. van; Wahlund, Karl-Gustav; Nilsson, Gunilla; Kok, Wim Th.

doi:10.1016/s0021-9673(01)01020-2

Cited by 67 publications

(44 citation statements)

References 35 publications

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“…Most often, retention is seen to decrease when sample concentration increases (i. e. positive dR/dpcP), as for particulate suspensions. This is the case for synthetic polymers, such as polystyrenesulfonates [8,15,22,24,26,33], polyvinylpyridines [26], or polyethylene oxides [32], but also for an amphiphilic graft copolymer [25], a protein [24], polysaccharides [24,34], and DNAs [20]. It has, however, been observed that the direction of variation of R with sample concentration depends on the eluent ionic strength, which influences the degree of interaction between macromolecules.…”

Section: Comparison With Experimental Datamentioning

confidence: 99%

Onset of sample concentration effects on retention in field‐flow fractionation

Martin¹,

Feuillebois²

2003

J of Separation Science

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In field-flow fractionation (FFF), when the sample amount is increased, a shift in retention occurs. Positive as well as negative deviations of the retention have been observed, depending on the type of sample and on the experimental conditions. Two concentration effects have been invoked to explain this retention shift: the viscosity effect (the velocity profile of the analyte suspension or solution is modified by the concentration dependence of the viscosity) and the mean distance effect (the mean distance of the analyte molecules or particles to the accumulation wall and the steady state cross-sectional concentration profile of the analyte are affected by concentration-dependent interactions between analyte molecules or particles). The general FFF retention equation has been solved in the case where the reciprocal of the viscosity and the FFF analyte-field interaction parameter, k, are linearly related to concentration. The two proportionality coefficients involved in these relationships are identified as the intrinsic viscosity and twice the second virial coefficient of the osmotic pressure, respectively. A general expression of the derivative of the analyte average relative velocity, R, with respect to the cross-sectional average analyte concentration, pcP, has been obtained as a function of these two coefficients, and of k o , the limit of k for infinite dilution. A survey of retention effects reported in FFF literature for various sample types and FFF methods is presented.

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Section: Comparison With Experimental Datamentioning

confidence: 99%

Onset of sample concentration effects on retention in field‐flow fractionation

Martin¹,

Feuillebois²

2003

J of Separation Science

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“…The technique minimizes or eliminates degradation and unwanted interaction [29] (i.e., low shear forces and small internal surface area). AF4 coupled with multi-angle light scattering (MALS) has already been applied successfully for M and root-meansquare radius (r rms ) determination of starches [5,7,15,16,30] (Table 1).…”

Section: Introductionmentioning

confidence: 99%

Development and evaluation of methods for starch dissolution using asymmetrical flow field-flow fractionation. Part II: Dissolution of amylose

2015

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In this paper, we investigate whether dissolution in water under autoclaving conditions (140 °C, 20 min) or in dimethyl sulfoxide, DMSO (100 °C, 1 h), is preferable for characterization of amylose. Two types of amylose, potato and maize, were dissolved either in water using an autoclave or in DMSO. On the aqueous solutions obtained, the extent of molecular dissolution of the sample (referred to as the dissolution yield) was determined by enzymatic analysis as well as the molecular properties, such as molar mass and root-mean-square radius, obtained with asymmetrical flow field-flow fractionation coupled to multi-angle light scattering and differential refractive index detection (AF4-MALS-dRI). The results showed that both dissolution methods are efficient at dissolving amylose. However, AF4-MALS-dRI analysis revealed substantial differences. Amylose aqueous solutions obtained by dissolution in DMSO were relatively stable over time, but the dissolution method in autoclave caused some degradation of the molecules, and their solutions display a high tendency to retrograde.

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“…The rms-MW plot indicated that the conformation of AMP, as expected, had a more compact structure than the lower molar mass components (AMY). Another study of AMP from waxy corn starch yielded an rms range of 250-1250 nm and molar masses from 10 7 to 10 9 g/mol [105]. In this case, the FFF separation occurred in the hyperlayer mode rather than the normal mode that we have described throughout this paper.…”

Section: Starchmentioning

confidence: 77%

Field‐flow fractionation of proteins, polysaccharides, synthetic polymers, and supramolecular assemblies

Williams¹,

Lee

2006

J of Separation Science

125

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This review summarizes developments and applications of flow and thermal field-flow fractionation (FFF) in the areas of macromolecules and supramolecular assemblies. In the past 10 years, the use of these FFF techniques has extended beyond determining diffusion coefficients, hydrodynamic diameters, and molecular weights of standards. Complex samples as diverse as polysaccharides, prion particles, and block copolymers have been characterized and processes such as aggregation, stability, and infectivity have been monitored. The open channel design used in FFF makes it a gentle separation technique for high- and ultrahigh-molecular weight macromolecules, aggregates, and self-assembled complexes. Coupling FFF with other techniques such as multiangle light scattering and MS provides additional invaluable information about conformation, branching, and identity.

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Retention behaviour of amylopectins in asymmetrical flow field-flow fractionation studied by multi-angle light scattering detection

Cited by 67 publications

References 35 publications

Onset of sample concentration effects on retention in field‐flow fractionation

Onset of sample concentration effects on retention in field‐flow fractionation

Development and evaluation of methods for starch dissolution using asymmetrical flow field-flow fractionation. Part II: Dissolution of amylose

Field‐flow fractionation of proteins, polysaccharides, synthetic polymers, and supramolecular assemblies

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