Retention mechanism and the role of the mobile phase in normal-phase separation on amino-bonded-phase columns

Snyder, Lloyd R.; Schunk, T. C.

doi:10.1021/ac00248a025

Cited by 89 publications

(26 citation statements)

References 23 publications

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“…In this paper, we use the widely accepted Snyder-Soczewinski model [26][27][28][29][30]. In the 1960s, Snyder developed a model for liquid solid chromatography [31][32][33][34].…”

Section: Theorymentioning

confidence: 99%

Retention mechanism of hypercrosslinked polystyrene silica hybrid phase in normal phase chromatography

Nedev

Lucy

2014

Journal of Chromatography A

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“…In this paper, we use the widely accepted Snyder-Soczewinski model [26][27][28][29][30]. In the 1960s, Snyder developed a model for liquid solid chromatography [31][32][33][34].…”

Section: Theorymentioning

confidence: 99%

Retention mechanism of hypercrosslinked polystyrene silica hybrid phase in normal phase chromatography

Nedev

Lucy

2014

Journal of Chromatography A

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“…The most commonly accepted retention mechanism is Snyder's model, which assumes the competitive adsorption between solutes and solvent molecules on active sites of the silica surface. Several studies have shown that this model is applicable for diol- [23,24], cyano- [23,25], and aminopropyl-bonded silica [26,27].…”

Section: Types Of Stationary Phasesmentioning

confidence: 99%

“…Retention is governed by the extent to which the analyte molecules displace the adsorbed solvent molecules on the surface of the stationary phase. This retention model based on adsorption was first proposed by Snyder [2][3][4][5] to describe retention on silica and alumina adsorbents and later extended to explain retention on polar bonded phases, such as diol-, cyano-, and amino-bonded silica. Snyder assumed a homogeneous surface so that adsorption energies for solute and solvent molecules are constant.…”

mentioning

confidence: 99%

Normal‐Phase HPLC

Liu

Vailaya

2006

HPLC for Pharmaceutical Scientists

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“…To the best of our knowledge there is no UHPLC method reported for the simultaneous estimation of PARA, ACF and CHZ in combined dosage forms. The structure of Paracetamol, Aceclofenac and Chlorzoxazone is represented in Figure 1 [2]. …”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Paracetamol, Aceclofenac and Chlorzoxazone in Pharmaceutical Dosage Form by UHPLC Method

Kb¹,

Murugan²,

Ezhilarasan³

et al. 2017

J Chromatogr Sep Tech

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Aim: UHPLC method was developed and validated for the determination of Paracetamol, Aceclofenac and Chlorzoxazone in pharmaceutical dosage form.Methods: Separation was performed in Agilent technologies 1220 infinity LC system equipped with open lab CDS Chemstation software using Eclipse plus C18 (50 × 4.6 mm id, 1.8 µm) column with mobile phase containing 0.05% orthophosphoric acid, adjusted to pH 6.0 with triethylamine and acetonitrile in the ratio of 70:30 v/v with a flow rate of 0.5 mL/min and detection wavelength 270 nm. Results:The method was found to be linear in the concentration range of 161.59-484.77 µg/mL for paracetamol, 49.69-149.09 µg/mL for aceclofenac and 124.81-374.43 µg/mL for chlorzoxazone with correlation coefficient of 0.999 for all the three drugs respectively. The %RSD of 0.57, 0.45 and 0.60 for intra-day and 0.96, 0.88 and 0.84 for inter-day precision, respectively for of Paracetamol, Aceclofenac and Chlorzoxazone suggest the precision of the method as all these values are less than 2%. The method has shown good, consistent recoveries for of Paracetamol (99.90-99.95%), Aceclofenac (99.83-99.92%) and Chlorzoxazone (99.39-99.59%) which are close to 100%. Conclusion:The method was found to be accurate, precise, specific, robust and linear for the determination of Paracetamol, Aceclofenac and Chlorzoxazone in pharmaceutical dosage form.

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Retention mechanism and the role of the mobile phase in normal-phase separation on amino-bonded-phase columns

Cited by 89 publications

References 23 publications

Retention mechanism of hypercrosslinked polystyrene silica hybrid phase in normal phase chromatography

Retention mechanism of hypercrosslinked polystyrene silica hybrid phase in normal phase chromatography

Normal‐Phase HPLC

Simultaneous Determination of Paracetamol, Aceclofenac and Chlorzoxazone in Pharmaceutical Dosage Form by UHPLC Method

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