Retention of cytoplasmic droplet by rat cauda epididymal spermatozoa after treatment with cytotoxic and xenobiotic agents

Akbarsha, Mohammad Abdulkader; Latha, P.; Murugaian, P.

doi:10.1530/jrf.0.1200385

Cited by 32 publications

(24 citation statements)

References 16 publications

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“…Another study revealed a positive association between OP metabolite levels and sex null and total aneuploidy frequencies in Mexican agricultural workers 19) . The sprayers in the present study had been exposed to fenitrothion most frequently, the testicular toxicity of which has not been reported, and often sprayed it together with DDVP, which was reported to be a possible testicular toxin 27,28) and to cause retention of cytoplasmic droplet and reduce sperm motility 29) in animal studies. But it is difficult to attribute the lower semen quality of the sprayer group to specific pesticides at this time.…”

Section: Discussionmentioning

confidence: 83%

“…Since they often sprayed the insecticides in narrow spaces such as under floors, they could potentially receive extensive exposure [22][23][24][25][26] . Despite the male reproductive toxicity of DDVP suggested in rodent studies [27][28][29] , no human data on exposed populations have been reported to date. As expected, there was great difficulty in conducting the semen study and genital organ examination of 'healthy' subjects, which we managed to overcome.…”

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confidence: 99%

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A Survey of Semen Indices in Insecticide Sprayers

Kamijima

Hibi

Gotoh

et al. 2004

Journal of Occupational Health

100

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A Survey of Semen Indices in InsecticideSprayers: Michihiro Kamijima, et al. Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine-This study aims at clarifying the semen indices of insecticide sprayers who are exposed mainly to organophosphorus and pyrethroid insecticides. Eighteen male sprayers out of 54 working for 9 companies in central Japan and 18 age-matched students or medical doctors as unexposed controls participated in detailed reproductive check-ups conducted in summer and the following winter. The sprayers were exposed to insecticides more in summer, the busiest season, than winter, the off-season (p<0.05). Erythrocyte true cholinesterase activities in the sprayers were lower than in the controls in summer (p<0.05), and decreased in significant association with the increase in exposure frequency. Testicular volumes in the sprayers tended to be smaller than in the controls (p=0.06). The serum testosterone concentration in winter in the sprayers was higher than in the controls (p<0.05), though luteinizing h o r m o n e a n d f o l l i c l e s t i m u l a t i n g h o r m o n e concentrations were not significantly different. The sperm counts and vitality were comparable between the groups, but detailed sperm motility analysis in summer revealed that the percentages of slow progressive and nonprogressive motile sperm were twice as high in the sprayers (p<0.05), and that of rapid progressive sperm tended to be lower (p=0.06). Such differences were not observed in winter. Differential sperm morphology counts showed that interaction of group and abstinence effects were significant in sperm Received Aug 19, 2003; Accepted Nov 28, 2003 Correspondence to: M. Kamijima, Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Aichi 466-8550, Japan (e-mail: kamijima@med.nagoya-u.ac.jp) with normal morphology and with head deformity only in the summer check-up. Despite possible inherent differences between the groups, the above seasondependent differences suggested that the observed lower semen quality in the sprayers was associated with pesticide spraying work.

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Section: Discussionmentioning

confidence: 83%

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confidence: 99%

A Survey of Semen Indices in Insecticide Sprayers

Kamijima

Hibi

Gotoh

et al. 2004

Journal of Occupational Health

100

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“…Dimethoate would be cytotoxic. This cytotoxic effect seems to be exerted by a phenomenon of germ cells necrosis (Akbarsha et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

Effect of Dimethoate (An Organophosphate Insecticide) on the Reproductive System and Fertility of Adult Male Rat

Ngoula¹

2014

American Journal of Pharmacology and Toxicology

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The pesticides are one of the most potentially harmful chemicals liberated in the environment in an unplanned manner. Dimethoate is widely used as a potent pesticide in many countries and has been shown to produce some adverse health effects. In the present study, we investigated the effect of dimethoate (an organophosphate insecticide) on the reproductive system and fertility of male rats and the possible mechanism underlining its action. Twenty four adult Wistar male rats were divided into 4 groups of 6 animals per group and force-fed with 0, 3.66, 5.50 and 11 mg kg −1 body weight of dimethoate for 90 days. At 80 days of treatment, each males was allows to mate with two primiparous cyclic females. The results showed a significant decrease (p<0.05) of body weight gain in rats gavaged with dimethoate compared to control. In addition, this insecticide at the doses of 3.66, 5.5 and 11 mg kg −1 caused a significant (p<0.05) increase of the relative weight of epididymis. Dimethoate at its highest dose caused a decrease in the relative weights of testes and ventral prostate. The doses 3.66, 5.50 and 11.00 mg kg −1 body weight of dimethoate caused a significant decrease (p<0.05) of the sperm concentration and motility. The level of protein and cholesterol in the serum and testes as well as the activity of prostatic acid phosphatase decreased significantly (p<0.05) in rats treated with dimethoate compared to control. Fertility, gestation and parturition indices as well as the litter size decreased significantly in animals treated with 5.50 and 11.00 mg kg −1 body weight of dimethoate compared to control. The testicular histology of animals treated with high doses of dimethoate generally showed span of Sertoli cells destruction and disorganization of the germinal epithelium. The lumen of seminiferous tubules of treated rats was generally poor in sperm. The results of this study confirmed that dimethoate seriously deteriorate the male reproductive system resulting in decreased fertility.

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“…The motility of spermatozoa may be compromised [Akbarsha et al 2000, Amann et al 2000, Thundathil et al 2001, or the spermatozoa with an attached CD may have a diminished ability to interact with the oviductal epithelium [Petrunkina et al 2001] and oocytes [Amann et al 2000, Thundathil et al 2001 in the female reproductive tract. The detrimental effects of attached CDs may stem from the production of reactive oxygen species, that have been associated with loss of functional competence in mammalian sperm [Aitken 1995].…”

Section: Introductionmentioning

confidence: 99%

High Resolution Light Microscopic Evaluation of Boar Semen Quality Sperm Cytoplasmic Droplet Retention in Relationship with Boar Fertility Parameters

Lovercamp

Safranski

Fischer

et al. 2007

Archives of Andrology

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The purpose of this study was to investigate the relationship between fertility and quantitative measures of boar semen quality, including various patterns of sperm cytoplasmic droplet (CD) retention, as determined by high power differential interference contrast (DIC) microscopy. A total of 116 ejaculates were collected from a nucleus herd of 18 Large White boars over an eight month period. Semen quality parameters were analyzed for each ejaculate by calculating the percentage of normal spermatozoa, spermatozoa possessing a CD in the proximal, distal, or distal midpiece reflex position, total spermatozoa with an attached cytoplasmic droplet, spermatozoa with non-CD related aberrations and total spermatozoa with abnormalities. Of the 116 ejaculates received, 71 ejaculates from 13 boars had corresponding fertility data from single-sire inseminations of multiparous sows. The fertility data included farrowing rate (FR) and total number born (TNB). The monthly FR encompassed one month before and one month after the date of semen collection. Detection of differences for fertility and semen quality parameters was performed by separating the boars into either an above-average or below-average group based on the mean FR (74.01 AE 1.43%) or TNB (12.34 AE 0.17) for the study. For FR, the boars in the below-average group had a significantly lower percentage of normal spermatozoa and significantly higher percentage of spermatozoa possessing distal CDs, total attached CDs and total abnormalities compared to the boars in the above-average group. Conversely, for TNB there were no significant differences between the above-and below-average groups for the semen quality parameters. These data suggest that the attached CD may negatively affect FR, but not TNB. The detection of relationships between the boar fertility parameters and the retention of the sperm CD after ejaculation, document the advantage of high power DIC microscopy in conventional semen evaluation.

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Retention of cytoplasmic droplet by rat cauda epididymal spermatozoa after treatment with cytotoxic and xenobiotic agents

Cited by 32 publications

References 16 publications

A Survey of Semen Indices in Insecticide Sprayers

A Survey of Semen Indices in Insecticide Sprayers

Effect of Dimethoate (An Organophosphate Insecticide) on the Reproductive System and Fertility of Adult Male Rat

High Resolution Light Microscopic Evaluation of Boar Semen Quality Sperm Cytoplasmic Droplet Retention in Relationship with Boar Fertility Parameters

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