Retention of insulin-like growth factor I bioactivity during the fabrication of sintered polymeric scaffolds

Clark, Amanda M.; Milbrandt, Todd A.; Hilt, J. Zach; Puleo, David A.

doi:10.1088/1748-6041/9/2/025015

Cited by 16 publications

(12 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Recent investigations demonstrated retention of IGF-I bioactivity during scaffold fabrication, which includes fusion of microspheres at 49°C. 29 Seeding BMCs onto the scaffold was performed in an effort to add mesenchymal cells to the implant site directly instead of waiting for cell recruitment and proliferation during recovery.…”

Section: Discussionmentioning

confidence: 99%

Treating Proximal Tibial Growth Plate Injuries Using Poly(Lactic-co-Glycolic Acid) Scaffolds

Clark

Hilt

Milbrandt

et al. 2015

BioResearch Open Access

Self Cite

View full text Add to dashboard Cite

Growth plate fractures account for nearly 18.5% of fractures in children. Depending on the type and severity of the injury, inhibited bone growth or angular deformity caused by bone forming in place of the growth plate can occur. The current treatment involves removal of the bony bar and replacing it with a filler substance, such as a free fat graft. Unfortunately, reformation of the bony bar frequently occurs, preventing the native growth plate from regenerating. The goal of this pilot study was to determine whether biodegradable scaffolds can enhance native growth plate regeneration following a simulated injury that resulted in bony bar formation in the proximal tibial growth plate of New Zealand white rabbits. After removing the bony bar, animals received one of the following treatments: porous poly(lactic-co-glycolic acid) (PLGA) scaffold; PLGA scaffold loaded with insulin-like growth factor I (IGF-I); PLGA scaffold loaded with IGF-I and seeded with autogenous bone marrow cells (BMCs) harvested at the time of implantation; or fat graft (as used clinically). The PLGA scaffold group showed an increased chondrocyte population and a reduced loss of the remaining native growth plate compared to the fat graft group (the control group). An additional increase in chondrocyte density was seen in scaffolds loaded with IGF-I, and even more so when BMCs were seeded on the scaffold. While there was no significant reduction in the angular deformation of the limbs, the PLGA scaffolds increased the amount of cartilage and reduced the amount of bony bar reformation.

show abstract

Section: Discussionmentioning

confidence: 99%

Treating Proximal Tibial Growth Plate Injuries Using Poly(Lactic-co-Glycolic Acid) Scaffolds

Clark

Hilt

Milbrandt

et al. 2015

BioResearch Open Access

Self Cite

View full text Add to dashboard Cite

show abstract

“…working at temperatures up to 60°C and in the presence of organic solvents, while here IGF-1 was dissolved in bi-distilled water and loaded on preformed microspheres at room temperature). 35 However, the retention of growth factor activity was indirectly verified, through the effect of loaded particles on cell response (both in vitro and in vivo ), as it will be detailed in the next paragraphs.…”

Section: Resultsmentioning

confidence: 99%

IGF-1 loaded injectable microspheres for potential repair of the infarcted myocardium

et al. 2020

View full text Add to dashboard Cite

The use of injectable scaffolds to repair the infarcted heart is receiving great interest. Thermosensitive polymers, in situ polymerization, in situ cross-linking, and self-assembling peptides are the most investigated approaches to obtain injectability. Aim of the present work was the preparation and characterization of a novel bioactive scaffold, in form of injectable microspheres, for cardiac repair. Gellan/gelatin microspheres were prepared by a water-in-oil emulsion and loaded by adsorption with Insulin-like growth factor 1 to promote tissue regeneration. Obtained microspheres underwent morphological, physicochemical and biological characterization, including cell culture tests in static and dynamic conditions and in vivo tests. Morphological analysis of the microspheres showed a spherical shape, a microporous surface and an average diameter of 66 ± 17µm (under dry conditions) and 123 ± 24 µm (under wet conditions). Chemical Imaging analysis pointed out a homogeneous distribution of gellan, gelatin and Insulin-like growth factor-1 within the microsphere matrix. In vitro cell culture tests showed that the microspheres promoted rat cardiac progenitor cells adhesion, and cluster formation. After dynamic suspension culture within an impeller-free bioreactor, cells still adhered to microspheres, spreading their cytoplasm over microsphere surface. Intramyocardial administration of microspheres in a cryoinjury rat model attenuated chamber dilatation, myocardial damage and fibrosis and improved cell homing. Overall, the findings of this study confirm that the produced microspheres display morphological, physicochemical, functional and biological properties potentially adequate for future applications as injectable scaffold for cardiac tissue engineering.

show abstract

“…H-Tecido de reparação neoformado, constituído principalmente de cartilagem hialina (Obj: 10x). Alguns trabalhos científicos demonstraram que o IGF-1 eleva a síntese de colágeno tipo 2 e proteoglicanos [1,11,25]. O colágeno tipo 2 é a base da cartilagem hialina e um importante componente da rede fibrilar de colágenos, que permite a cartilagem aprisionar o proteoglicano formado, proporcionando força de tensão ao tecido [21].…”

Section: Discussionunclassified

“…A utilização do enxerto osteocondral autógeno possibilita a implantação da cartilagem hialina de forma direta no interior do defeito, em um procedimento único, permitindo o movimento articular precoce e o apoio do membro lesionado [7,14,24]. Alguns fatores de crescimento têm sido avaliados em associação aos tecidos enxertados, tendo destaque para a utilização do fator de crescimento semelhante à insulina tipo 1 (IGF-1), que estimula a proliferação de vários tipos celulares e auxilia na formação de tecido novo [1,11,12,15,16,20,25].…”

Section: Introductionunclassified

Autogenous Osteochondral Graft Associated with IGF-1 in Induced Articular Cartilage Lesion in Rabbits

Kawamoto

Muzzi

Junior

et al. 2018

Acta Scientiae. Vet.

View full text Add to dashboard Cite

Background: Articular cartilage has a limited capacity for regeneration and of the various treatments proposed, none have reached appropriate therapeutic effectiveness. This study aimed to evaluate autogenous osteochondral grafts in intact or macerated format, in association with or without insulin-like growth factor type-1 (IGF-1) in the repair of osteochondral defects induced in the femoral trochlear groove of rabbits.Materials, Methods & Results: Seventeen healthy White New Zealand rabbits were selected for this study. The rabbits were female, six months old, and had an average body weight of 4.5 kg. All 34 stifle joints were subjected to autogenous osteochondral grafting in the femoral trochlear groove. The joints were divided into four groups designated as intact osteochondral graft with IGF-1 (INT + IGF), intact osteochondral graft with physiological solution (INT + FIS), macerated osteochondral graft with IGF-1 (MAC + IGF), and macerated osteochondral graft with physiological solution (MAC + FIS). Serial evaluations were performed by orthopedic and radiographic examination. After 6 and 12 weeks postoperatively, the grafted area was subjected to macroscopic, histological, and immunohistochemical analyses. Although no statistically significant differences were found between the groups in relation to clinical, macroscopic, histological, and immunohistochemical aspects, a tendency of IGF-1 to promote tissue repair was evident. In the radiographic evaluation, the articular surface and the recipient site in both groups with IGF-1 showed significantly more effective filling (P ≤ 0.05). Regardless of the group, collagen type 2 production, as assessed by immunohistochemistry, was found to be appropriate on the grafted articular surface.Discussion: In extensive cartilage lesions, the use of intact osteochondral grafts may be infeasible due to donor site morbidity. An alternative is the use of macerated osteochondral grafts, which cover a larger area and act as a support and cellular source in the repair process. Growth factors have been evaluated in association with grafted tissues to aid tissue repair, and IGF-1 is currently prominent. In the radiographic analysis of the present study, when comparing sites subjected to osteochondral grafting, presence of the whole graft evidenced adequate local filling in all groups. However, graft integration was apparently rapid and effective in the INT + IGF and MAC + IGF groups from the sixth and ninth weeks of the procedure, respectively. In the macroscopic evaluation at the twelfth week, graft integration with the original cartilaginous tissue was more evident, especially in both groups treated with IGF-1. It is likely that the property of IGF-1 to increase chondrogenesis in the cartilage repair of articular lesions in vivo may have contributed to these results in radiographic and macroscopic examinations. Histological examination showed no significant difference between groups in the same period of time; however, it was observed that addition of IGF-1 promoted a more evident tissue reaction and cellular activation, potentiating the process of reabsorption and repair in the grafted tissue. Immunohistochemical analysis showed similar immunoreactivity for collagen type 2 in all groups as early as the sixth week. However, a small portion of these tissues cannot be considered true hyaline cartilage due to the absence of some typical features. In summary, addition of IGF-1 to the autogenous osteochondral graft seemed to stimulate reabsorption and replacement processes in the grafted tissue. The grafts showed adequate ability to repair articular cartilage, displaying formation of collagen type 2 similar to that in the original tissue.

show abstract

Retention of insulin-like growth factor I bioactivity during the fabrication of sintered polymeric scaffolds

Cited by 16 publications

References 56 publications

Treating Proximal Tibial Growth Plate Injuries Using Poly(Lactic-co-Glycolic Acid) Scaffolds

Treating Proximal Tibial Growth Plate Injuries Using Poly(Lactic-co-Glycolic Acid) Scaffolds

IGF-1 loaded injectable microspheres for potential repair of the infarcted myocardium

Autogenous Osteochondral Graft Associated with IGF-1 in Induced Articular Cartilage Lesion in Rabbits

Contact Info

Product

Resources

About