Retention of microbial cells in substratum surface features of micrometer and sub-micrometer dimensions

Whitehead, Kathryn A.; Colligon, J.S.; Verran, Joanna

doi:10.1016/j.colsurfb.2004.11.010

Cited by 277 publications

(206 citation statements)

References 39 publications

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“…Whitehead et al further supported this view when they found higher bacterial counts on substrates that contained 2 µm diameter pits as opposed to those in the range of 0.5 µm. 75 Xu et al compared bacterial adhesion between two polyurethane surfaces of the same chemistry; one was smooth and the other contained oriented protrusions or pillars (400 nm diameter × 650 nm height). 76 They found biofilm formation significantly less for both S. epidermidis and S. aureus when using the highly textured polyurethane.…”

Section: Nanostructured Surfacesmentioning

confidence: 99%

Decreased bacteria activity on Si3N4 surfaces compared with PEEK or titanium

Gorth¹,

Puckett²,

Ercan³

et al. 2012

IJN

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A significant need exists for orthopedic implants that can intrinsically resist bacterial colonization. In this study, three biomaterials that are used in spinal implants -titanium (Ti), polyether-ether-ketone (PEEK), and silicon nitride (Si 3 N 4 ) -were tested to understand their respective susceptibility to bacterial infection with Staphylococcus epidermidis, Staphlococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Enterococcus. Specifically, the surface chemistry, wettability, and nanostructured topography of respective biomaterials, and the effects on bacterial biofilm formation, colonization, and growth were investigated. Ti and PEEK were received with as-machined surfaces; both materials are hydrophobic, with net negative surface charges. Two surface finishes of Si 3 N 4 were examined: as-fired and polished. In contrast to Ti and PEEK, the surface of Si 3 N 4 is hydrophilic, with a net positive charge. A decreased biofilm formation was found, as well as fewer live bacteria on both the as-fired and polished Si 3 N 4 . These differences may reflect differential surface chemistry and surface nanostructure properties between the biomaterials tested. Because protein adsorption on material surfaces affects bacterial adhesion, the adsorption of fibronectin, vitronectin, and laminin on Ti, PEEK, and Si 3 N 4 were also examined. Significantly greater amounts of these proteins adhered to Si 3 N 4 than to Ti or PEEK. The findings of this study suggest that surface properties of biomaterials lead to differential adsorption of physiologic proteins, and that this phenomenon could explain the observed in-vitro differences in bacterial affinity for the respective biomaterials. Intrinsic biomaterial properties as they relate to resistance to bacterial colonization may reflect a novel strategy toward designing future orthopedic implants.

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Section: Nanostructured Surfacesmentioning

confidence: 99%

Decreased bacteria activity on Si3N4 surfaces compared with PEEK or titanium

Gorth¹,

Puckett²,

Ercan³

et al. 2012

IJN

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“…Having said this, they also correctly state that these surfaces are not fully stable and as a result require further investigation. Ion sputtering technologies have also been implemented for the capture and immobilization of bacterial cells, as shown in the research conducted by Whitehead et al [174,175]. This research on the capture and immobilization of bacterial cells has shown how micrometre-scale features on silicon and titanium can give rise to controlled bacterial adhesion and growth in specific, discrete areas.…”

Section: Ion Beam and Electron Beam Processingmentioning

confidence: 98%

“…This ultimately indicates that EB processing can be used in applications such as biosensors and tissue engineering devices. Ion beam technology has also been employed to produce various topographies and surface chemistries, mainly through the use of ion beam sputtering [173][174][175] but ion beam etching has also been effectively employed [176]. Trujillo et al [173] have shown that ion beam sputtered silver-doped hydroxyapatite on titanium can have the advantage of hindering Staphylococcus epidermis and Pseudomonas aeruginosa growth.…”

Section: Ion Beam and Electron Beam Processingmentioning

confidence: 99%

Surface Treatments to Modulate Bioadhesion: A Critical Review

Waugh¹,

Toccaceli²,

Gillett³

et al. 2016

rev adhes adhesives

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On account of the recent increase in importance of biological and microbiological adhesion in industries such as healthcare and food manufacturing many researchers are now turning to the study of materials, wettability and adhesion to develop the technology within these industries further. This is highly significant as the stem cell industry alone, for example, is currently worth £3.5 million in the United Kingdom (UK) alone. This paper reviews the current state-of-the-art techniques used for surface treatment with regards to modulating biological adhesion including laser surface treatment, plasma treatment, micro/nano printing and lithography, specifically highlighting areas of interest for further consideration by the scientific community. What is more, this review discusses the advantages and disadvantages of the current techniques enabling the assessment of the most attractive means for modulating biological adhesion, taking in to account cost effectiveness, complexity of equipment and capabilities for processing and analysis.

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“…Also, Anselme [49] showed a relationship between roughness and adherent bacteria number on polyurethane-coated glass plates preconditioned in seawater. Whitehead et al [50] & Campoccia et al [51] studied this subject by testing different pits with specific diameter and depth, whereas in our assay, the marks were irregular on the plates and the results expressed as displacement and not bacteria number; in the first study, there was an increase of adherent bacteria number on rough surfaces, especially on the bottom of crevices [52], although the lowest number was obtained on the larger pit, but in the second one, no differences were observed. In addition, some authors have proposed that bacterial response to nanometer scale roughness is mediated by structures such as fimbriae [48,49].…”

Section: Discussionmentioning

confidence: 99%

Effect of Different Culture Conditions on the (Twitching) Displacement of Photobacterium Damselae subsp. Piscicida

Vega¹,

Jose²,

Bravo³

et al. 2017

JBMOA

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Photobacterium damselae subsp. piscicida (Phdp), etiological agent of photobacteriosis, is a non-motile bacterium. Notwithstanding, it is able to move on solid surfaces. The presence of pili-like structures on the bacterial surface has been recently described and being associated with twitching displacement by our team. The objective of this study was to modify some conditions (temperature, pH, concentration of nutrients, salinity, solidification of the medium, surface roughness and presence of target cells of Phdp) to determine which ones promote motility on solid surfaces.Lower temperatures (≤ 18˚C), alkaline and acidic environments and lacking salinity (<0.5%) media had a negative effect on cell motility, while nutrient limitation did not affect the bacterial response. The best twitching motility was observed when Phdp was inoculated in medium with 0.2% agar and pH 7.0. Also, interesting results were obtained on rough surfaces or by the addition of cellular debris of SAF-1 cell line to the medium.According to the results, the "new" composition of the "twitching displacement" medium could be as follows: 3.7% (w/v) brain-heart infusion broth (BHIB) supplemented with about 1.0% (w/v) NaCl and 0.2% (w/v) agar, with a final pH of 7.0, and maybe, scrape petri dishes and add debris of SAF-1 cells 1:2-factor dilution to medium. If bacterial strains are incubated at 22-25˚C for three days, a good response should be achieved in terms of fimbriae production.

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Retention of microbial cells in substratum surface features of micrometer and sub-micrometer dimensions

Cited by 277 publications

References 39 publications

Decreased bacteria activity on Si3N4 surfaces compared with PEEK or titanium

Decreased bacteria activity on Si3N4 surfaces compared with PEEK or titanium

Surface Treatments to Modulate Bioadhesion: A Critical Review

Effect of Different Culture Conditions on the (Twitching) Displacement of Photobacterium Damselae subsp. Piscicida

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