IntroductionHealth workforce reform was undertaken in Guinea in 2016 following the Ebola outbreak to overcome the decades-long shortage and maldistribution of HCWs. This study aims to assess the effects of this programme on local health systems and its influence on HCWs turnover in rural Guinea. MethodsAn exploratory study design using a mixed-method approach was conducted in five rural health districts. Data were collected through a semi-structured questionnaire, in-depth interviews guides and documentary review. ResultsOut of the 611 HCWs officially deployed in the selected districts, 600 (98%) took-up duties. Female HCWs (64%), assistant nurses (39%), nurses (26%) and medical doctors (20%) represented the majority. Findings showed that 69% of HCWs were posted in health centres; the majority of which were medical doctors, nurses and midwives. The deployment has reportedly enhanced quality and timely data reporting. However, challenges were faced by local health authorities in the posting of HCWs including the unfamiliarity of some with primary healthcare delivery, collaboration conflicts between HCWs and the high feminization of the recruitment. One year after their deployment, 31% of the HCWs were absent from their posts. This included 59% of nurses, 29% of medical doctors and 11% of midwives. Main reasons for absenteeism were unknown (51%), continuing training (12%), illness (10%) and maternity leave (9%). Finding showed a confusion of roles and responsibilities between national and local actors in the management of HCWs; which was accentuated by lack of policy documents. ConclusionThe post-Ebola healthcare workers policy appears to have been successfully positive in the redistribution of HCWs, quality improvement of staffing levels in peripheral healthcare facilities, and the enhancing of district health offices capacities. However, greater attention should be given to the development of policy guidance documents with the full participation of all actors and a clear distinction of their roles and responsibilities for improved implementation and efficacy of this programme.