Retentive efficacy, antimicrobial and cytotoxicity comparisons between different types of commercial and experimental denture adhesives with antifungal action

Abstract

The effect of the addition of nystatin and an alternative antifungal derived from pyrazoles in different commercial denture adhesives on their retentive efficacy, cytotoxicity and antimicrobial activity against Candida albicans was evaluated. Commercial denture adhesives were prepared with the inclusion of nystatin and 3,5-diaryl-4,5-dihydro-1H-pyrazole-1-carboximidamide (pyrazole) in three concentrations: 23.78 %w/w, 3.02 %w/w, and 0.31 %w/w (0.015 g, 0.0015 g, and 0.00015 g, respectively). The retentive efficacy was tested observing the influence of the medium, type of commercial denture type and the test condition (dipping). The antifungal action through disk diffusion and direct contact tests at 1, 4, 8 and 12 h and cytotoxic activity was evaluated in mouse fibroblasts (NIH/3T3) by the MTT reduction colorimetric assay. The addition of pyrazole and nystatin in commercial denture adhesives did not affect retentive efficacy rates and enhanced antifungal actions against Candida albicans. Results show a possibility of using denture adhesives as a delivery system for commercial antifungals (Nystatin) or pyrazole, with the second concentration (1,560 µg-3.02 %w/w) as the most efficient.

Biomaterials for the Prevention of Oral Candidiasis Development

“…have been shown to form biofilms on the surface of various medical devices made of PMMA, silicone elastomer, polyurethane, polyvinyl chloride, polypropylene, and polystyrene, among others [27]. Additionally, the use of denture adhesives, besides their functional and psychological advantages, has been reported to predispose wearers to oral candidiasis [134]. Hence, these two elements could hold great improvement potential in synergy with fungicidal or fungistatic materials [135].…”

“…Alternatively, Namangkalakul et al [22] stated that high-molecular-weight watersoluble chitosan can serve as an antifungal adhesive to prevent and treat denture stomatitis. Besides, denture adhesives could be used as delivery systems for antifungal agents without affecting their adhesion capacity [134].…”