Reticular and erosive oral lichen planus have a distinct metabolomic profile: A preliminary study using gas chromatography‐mass spectrometry

Cruz, Aline Fernanda; Vitório, Jéssica Gardone; Duarte‐Andrade, Filipe Fideles; Diniz, Marina Gonçalves; Canuto, Gisele André Baptista; Toledo, Juliano Simões de; Fonseca, Felipe Paiva; Fernandes, Ana Paula; André, Leiliane Coelho; Gomes, Carolina Cavaliéri; Gomez, Ricardo Santiago

doi:10.1111/jop.12842

Cited by 4 publications

(2 citation statements)

References 22 publications

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“…Contrary to other studies, this one did not use saliva as a sample but formalin-fixed and paraffin-embedded tissue of erosive and reticular oral lichen planus. The method of choice was GC-MS [55].…”

Section: Oral Lichen Planusmentioning

confidence: 99%

Applications of Mass Spectrometry in Dentistry

et al. 2023

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Mass Spectrometry (MS) is one of the fastest-developing methods in analytical instrumentation. As a highly sensitive, universal detector, it can identify known and unknown compounds, which can indeed be found in a minimal concentration. This review aims to highlight the significant milestones in MS applications in dentistry during recent decades. MS can be applied in three different fields of dentistry: (1) in research of dental materials and chemical agents, (2) in laboratory analysis of biospecimens, and (3) as a real-time diagnostic tool in service of oral surgery and pathology. MS applications on materials and agents may focus on numerous aspects, such as their clinical behavior, possible toxicity, or antimicrobial properties. MS is also a valuable, non-invasive tool for biomarkers’ detection in saliva and has found great application in -omics technologies as it achieves efficient structure-finding in metabolites. As metabolites are located beyond the central dogma, this technique can provide a complete understanding of cellular functions. Thus, it is possible to determine the biological profile in normal and pathological conditions, detect various oral or systematic diseases and conditions, and predict their course. Lastly, some promising advances concerning the surgical approach to potentially oral malignant or malignant disorders exist. This breakthrough method provides a comprehensive approach to dental materials research and biomarker discovery in dental and craniofacial tissues. The current availability of various ‘OMIC’ approaches paves the way for individualized dentistry and provides suggestions for clinical applications in the point-of-care hubs.

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Section: Oral Lichen Planusmentioning

confidence: 99%

Applications of Mass Spectrometry in Dentistry

et al. 2023

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“…Cruz et al. ( 9 )performed metabolomics analysis on erosive and reticulated OLP tissue samples, and found the capability of this technology to distinguish OLP tissue typing. Wang et al.…”

Section: Introductionmentioning

confidence: 99%

Metabolomics and Transcriptomics Analysis on Metabolic Characteristics of Oral Lichen Planus

et al. 2021

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ObjectiveTo explore metabolic biomarkers related to erosive and reticulated oral lichen planus (OLP) by non-targeted metabolomics methods and correlate metabolites with gene expression, and to investigate the pathological network pathways of OLP from the perspective of metabolism.MethodsA total of 153 individuals were enrolled in this study, including 50 patients with erosive oral lichen planus (EOLP), 51 patients with reticulated oral lichen planus (ROLP), and 52 healthy controls (HC). The ultra-high-performance liquid chromatography quadrupole-Orbitrap high-resolution accurate mass spectrometry (UHPLC/Q-Orbitrap HRMS) was used to analyze the metabolites of 40 EOLP, 40 ROLP, and 40 HC samples, and the differential metabolic biomarkers were screened and identified. The regulatory genes were further screened through the shared metabolites between EOLP and ROLP, and cross-correlated with the OLP-related differential genes in the network database. A “gene-metabolite” network was constructed after finding the key differential genes. Finally, the diagnostic efficiency of the biomarkers was verified in the validation set and a diagnostic model was constructed.ResultCompared with HC group, a total of 19 and 25 differential metabolites were identified in the EOLP group and the ROLP group, respectively. A total of 14 different metabolites were identified between EOLP and ROLP. Two diagnostic models were constructed based on these differential metabolites. There are 14 differential metabolites shared by EOLP and ROLP. The transcriptomics data showed 756 differentially expressed genes, and the final crossover network showed that 19 differential genes were associated with 12 metabolites. Enrichment analysis showed that alanine, aspartate and glutamate metabolism were closely associated with the pathogenesis of OLP.ConclusionThe metabolic change of different types of OLP were clarified. The potential gene perturbation of OLP was provided. This study provided a strong support for further exploration of the pathogenic mechanism of OLP.

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Effects of Stress on Biological Characteristics and Metabolism of Periodontal Ligament Stem Cells of Deciduous Teeth

Li,

Dai

et al. 2024

International Dental Journal

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Reticular and erosive oral lichen planus have a distinct metabolomic profile: A preliminary study using gas chromatography‐mass spectrometry

Cited by 4 publications

References 22 publications

Applications of Mass Spectrometry in Dentistry

Applications of Mass Spectrometry in Dentistry

Metabolomics and Transcriptomics Analysis on Metabolic Characteristics of Oral Lichen Planus

Effects of Stress on Biological Characteristics and Metabolism of Periodontal Ligament Stem Cells of Deciduous Teeth

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