Reticulated platelet values in normal and thrombocytopenic neonates

Peterec, Steven M.; Brennan, Sharon A.; Rinder, Henry M.; Wnek, Judith L.; Beardsley, Diana S.

doi:10.1016/s0022-3476(96)70253-6

Cited by 67 publications

(28 citation statements)

References 24 publications

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“…Richards & Baglin (1995) studied 79 thrombocytopenic patients (25 with immune thrombocytopenia and a normal bone marrow, 31 with bone marrow aplasia, and seven with consumptive coagulopathy) and found that the patients with increased peripheral platelet destruction had a higher RP% than those with decreased platelet production. Similar results have been reported by other groups (Watanabe et al, 1995;Chavda et al, 1996;Peterec et al, 1996;Koike et al, 1998;Saxon et al, 1998;Stohlawetz et al, 1999). It has even been postulated that the RP% alone has an even better predictive value than PA-IgG measured with flow cytometry for the diagnosis of autoimmune thrombocytopenia .…”

Section: Rp In Healthy Controls and Patientssupporting

confidence: 87%

Autoimmune Thrombocytopenia

2017

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Section: Rp In Healthy Controls and Patientssupporting

confidence: 87%

Autoimmune Thrombocytopenia

2017

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“…Quantification of reticulated PLTs as an index of neonatal thrombopoiesis may be one option. 31,32 Evaluation of circulating TPO alone may have limited diagnostic value; 33 however, the combination of several approaches such as evaluation of plasma TPO, glycocalicin, and megakaryocyte cultures may help to distinguish mechanisms underlying neonatal thrombocytopenia. 34 Overall, thrombocytosis was common and, in noninfected AGA infants, it was more prevalent than thrombocytopenia, and varied inversely to postconceptional age, with a peak incidence at 35 weeks CGA.…”

Section: Discussionmentioning

confidence: 99%

Patterns of Thrombocytosis and Thrombocytopenia in Hospitalized Neonates

McPherson

Juul

2004

J Perinatol

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We sought to identify changes in platelet (PLT) counts over time, and to evaluate the patterns of thrombocytopenia and thrombocytosis in hospitalized infants 23.8 weeks to term gestation. STUDY DESIGN:Neonates were divided into four gestational age groups and their PLT counts were retrospectively compared for prevalence of thrombocytopenia, thrombocytosis, and associated conditions. RESULTS:Postconceptional age, postnatal age, and sepsis (among other factors) affected PLT counts. When counts from noninfected appropriately grown infants were evaluated, the risk of thrombocytopenia and thrombocytosis were highest in the most preterm infants, and these risks changed with corrected gestational age. PLT counts increased weekly over the first 4 weeks of life for all but the most preterm infants. CONCLUSIONS:These data characterize the incidence of thrombocytopenia and thrombocytosis across a wide range of gestational ages and show that, even in noninfected neonates, these conditions are common, and risk decreases with increasing maturity. The age-related changes in PLT patterns may reflect maturation of platelet regulation.

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“…Kienast and Schmitz initiated a breakthrough in the field when they described a flow cytometric technique for analyzing retPLT, based on RNA staining by thiazole orange [15]. In subsequent years, several research groups published their findings in a wide variety of conditions like thrombocytopenia [16][17][18][19][20][21], thrombocytosis [22,23], after stem cell transplantation [24][25][26][27], hereditary platelet diseases [28,29], thrombo-embolic disorders [30,31], kidney disease [32][33][34], preeclampsia [35], hyperthyroidism [36] and in healthy and thrombocytopenic neonates [37,38]. The overall conclusion from these studies is that retPLT in blood represent a useful non-invasive marker of megakaryopoietic activity in the bone marrow.…”

Section: Reticulated Platelet Methods -Flow Cytometrymentioning

confidence: 99%

Reticulated platelets: analytical aspects and clinical utility

Hoffmann

2014

Clinical Chemistry and Laboratory Medicine (CCLM)

139

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Reticulated platelets are immature platelets circulating in blood; they reflect the activity of megakaryopoiesis in the bone marrow. Therefore, they can be used as a non-invasive test in patients with thrombocytopenia in various clinical conditions. The preferred method of analysis is by flow cytometry. However, there is an evident lack of analytical standardization, making it difficult to compare results obtained in different laboratories. Currently, two types of hematology analyzers are on the market offering fully automated measurement of reticulated or immature platelets: the high end analyzers manufactured by Sysmex (XE-and XN-series) and Abbott (CELL-DYN Sapphire). Although the methods are essentially different and cannot be used interchangeably, both have been proven to have clinical utility. Reticulated or immature platelet assays are useful for the differential diagnosis of thrombocytopenia and for monitoring bone marrow recovery after chemotherapy or stem cell transplantation. These assays may aid clinicians in platelet transfusion decisions when recovery from thrombocytopenia is imminent. In addition, preliminary findings indicate that there is a rationale for reticulated or immature platelets for risk stratification in acute coronary syndromes and for monitoring the effect of treatment with antiplatelet drugs in patients with coronary artery diseases. The aim of this paper is to present the present technology available for measuring reticulated platelets as well as an overview of the current status of clinical application. This overview also indicates that more research is needed before reticulated or immature platelet assays can be applied in other clinical conditions than thrombocytopenia and after transplantation.

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Reticulated platelet values in normal and thrombocytopenic neonates

Cited by 67 publications

References 24 publications

Autoimmune Thrombocytopenia

Autoimmune Thrombocytopenia

Patterns of Thrombocytosis and Thrombocytopenia in Hospitalized Neonates

Reticulated platelets: analytical aspects and clinical utility

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