Reticulated Platelets and Platelet Reactivity in Renal Transplant Recipients Receiving Antiplatelet Therapy

Zanazzi, M.; Cesari, Francesca; Rosso, Gabriele; Farsetti, Silvia; Caroti, Leonardo; Gori, Anna Maria; Cerini, Gabriele; Cioni, Gabriele; Bertoni, E.; Abbate, Rosanna; Salvadori, M.

doi:10.1016/j.transproceed.2010.03.042

Cited by 11 publications

(6 citation statements)

References 8 publications

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“…IPF was assessed in patients who underwent renal transplantation; the Sysmex 2100 XE was also the device used in this study [24]. A higher IPF was detected in transplant recipient group when compared to a healthy control group, IPF values for both groups were not reported.…”

Section: Discussionmentioning

confidence: 99%

“…high platelet turnover) has been explained by increased generation of new platelets that are unaffected by antiplatelet therapy, possibly because of the uninhibited activity of COX1, and COX2 in the newly formed platelets [13,14,[20][21][22][23][24][25][26]. Recent studies showing that platelet mRNA is actively spliced and translated in response to platelet stimulation [27,28] also raises the possibility that prothrombotic pathways may also be activated in this platelet population despite partial blockade of P2Y12 by the active metabolite of clopidogrel.…”

Section: Discussionmentioning

confidence: 99%

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Immature platelet fraction (IPF) determined with an automated method predicts clopidogrel hyporesponsiveness

Ibrahim

Nadipalli

DeLao

et al. 2011

J Thromb Thrombolysis

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The mechanisms for the variability in antiplatelet effects of clopidogrel are not elucidated entirely. Immature (reticulated) platelets may modulate the antiplatelet effects of clopidogrel but must be measured using flow cytometry. Whether new automated detection techniques yield similar results is not known. The objectives of the study to evaluate the role of immature platelets assessed by an automated method in response to the antiplatelet effects of clopidogrel. Twenty-nine healthy volunteers had platelet studies performed before and 1 week after 75 mg daily dosing of clopidogrel. Immature platelet fraction (IPF) was determined using an automated particle counter. Subjects were stratified into tertiles based on the IPF. Platelet studies included light transmission aggregometry (LTA), and vasodilator stimulated phosphoprotein phosphorylation (VASP-P) determined by platelet reactivity index (PRI). Baseline platelet aggregation responses to 2, 5 and 20 μM ADP, were similar in all three tertiles, however they were greater in the upper than in the lower tertile of immature platelets after clopidogrel in response to 5 μM ADP (54% vs. 23%, P = 0.02), with concordant trends for the other two concentrations. PRI was also greater in the upper tertile after clopidogrel (71.2% vs. 57.8%, P = 0.04). The frequency of clopidogrel hyporesponsiveness (aggregation >50% in response to 5 μM of ADP) was also higher in the upper tertile when compared to lower tertile, (60%) versus (10%) respectively (P = 0.02). [corrected]. Immature platelets measured using an automated method, are associated with impaired response to antiplatelet effects of clopidogrel.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Immature platelet fraction (IPF) determined with an automated method predicts clopidogrel hyporesponsiveness

Ibrahim

Nadipalli

DeLao

et al. 2011

J Thromb Thrombolysis

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“…Increased circulating RPs has been proposed as an important mechanism associated with cardiovascular risk in these patients [20]. A significant association between RP count and platelet function supports the role for platelets in cardiovascular complications in kidney transplantation [58].…”

Section: Kidney Dysfunctionmentioning

confidence: 94%

“…It has been suggested that RPs are present in inflammation and interact with leukocytes and endothelial cells [19]. Moreover, RPs have reduced response to antiplatelet drugs, ie, aspirin, possibly due to their greater reactivity and impaired cyclooxygenase-1 and -2 inhibition [20].…”

Section: Properties Of Reticulated Plateletsmentioning

confidence: 99%

Clinical applicability of reticulated platelets

Dusse

Freitas

2015

Clinica Chimica Acta

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“…Степень подавления агрегации тромбоцитовпри изолированномпримененииаспирина (ингибитора циклооксигеназы и синтезатромбоксана А2) в зависимости от содержания РТ исследовали в группе здоровых доноров [10] и у больныхсо стабильнойстенокардией [10,27], диабетом [20] и перенесших трансплантацию почки [28]. Во всех работах повышение содержания РТ было ассоциировано с уменьшением антиагрегантногодействияаспирина.…”

Section: ретикулярные тромбоциты и эффективность антитромбоцитарных препаратовunclassified

Reticulated platelets – a new risk factor of atherothrombosis?

et al. 2021

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In this review we described the properties of reticulated platelets (RP) and showed how variations of their content might influence platelet activity, efficacy of antiplatelet drugs and the rate of thrombotic events in patients with cardiovascular diseases. RP represent a minor platelet fraction containing residual RNA from megakaryocytes. Platelets have no nucleus and do not synthesize RNA de novo, and RNA of megakaryocytic origin is destroyed during their circulation. That is why only recently produced “young” platelets contain RNA. In healthy donors RP are identified by staining with the RNA specific fluorescent dyes by flow cytofluorimetry or using standard protocols in modern flow haematological analyzers. RP content in blood reflects the level of thrombocytopoesis in the bone marrow. RP on average amounted from 3 to 10% of all platelets in the circulation depending on the method applied for their determination. RP absolute amount and/or their percentage is changed in haematological diseases associated with the alterations of megakaryocyte productive properties. RT measurements in patients with cardiovascular diseases have shown that their content is increased in acute coronary syndrome patients. RP are larger and functionally more active in comparison with not reticulated forms. They more frequently incorporate into the platelet aggregates and contain more intracellular granules. Increase of RT content in the circulation correlates with the increase of the average size and functional activity in the whole platelet population. High RP content in patients with cardiovascular diseases reduces antiaggregative effects of aspirin and P2Y12 APD receptor antagonists and increases the risk of atherothrombotic events.

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Reticulated Platelets and Platelet Reactivity in Renal Transplant Recipients Receiving Antiplatelet Therapy

Abstract: We documented the presence of higher concentrations of RPs in transplant recipients compared with a control population, and a significant association between RPs and platelet function.

Cited by 11 publications

References 8 publications

Immature platelet fraction (IPF) determined with an automated method predicts clopidogrel hyporesponsiveness

Immature platelet fraction (IPF) determined with an automated method predicts clopidogrel hyporesponsiveness

Clinical applicability of reticulated platelets

Reticulated platelets – a new risk factor of atherothrombosis?

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