Reticulocyte count in red‐blood‐cell units stored in <scp>AS</scp>‐1

Urbina, Adriana; Palomino, Fernando

doi:10.1111/vox.12011

Cited by 1 publication

(4 citation statements)

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“…When the aliquots taken at different blood bank storage times were compared, it was observed that the reticulocyte disappearance rate increased (shorter disappearance times) for RBC units having longer storage times in a glucose-rich medium such as AS-1. Since there was no difference between these aliquots regarding hemolysis, one plausible explanation is that the maturation of reticulocytes to discocytes occurred somewhat during storage in blood bank conditions 9 . Nevertheless, the maturation rate during storage at 4 °C appears to be much lower than that at 37 °C as reticulocytes were present even in aliquots taken from refrigerated RBC units after the sixth week of storage.…”

Section: Discussionmentioning

confidence: 93%

“…Differential centrifugation, density gradient centrifugation, and elutriation attain reticulocyte concentrations of 2.6%, 14 7–13% 14 and 4–5%,15, 16 respectively, while immunomagnetic separation using anti-CD71 antibodies attains concentrations greater than 90% 8, 15. However, none of these methods have been used in samples from RBC units that have been obtained by the centrifugation of whole blood units and have lower reticulocyte concentrations than those observed in venous blood 9 . In this study, we used a Percoll density gradient centrifugation technique with reticulocyte-poor samples due to the standard blood bank processing to obtain RBC units; this technique obtained inoculums for culturing with reticulocyte counts close to those of fresh peripheral venous blood.…”

Section: Discussionmentioning

confidence: 99%

“…Considering that the reticulocyte count in blood bank RBC units is lower than in peripheral blood and the number decreases further during refrigerated storage as we have previously reported, 9 a reticulocyte-enrichment procedure was designed to obtain culture inoculums with a reticulocyte count not below that usually expected in peripheral venous blood. Thus, before inoculation in culture bottles, reticulocyte-enriched fractions were obtained from RBC unit aliquots using Percoll density gradient separation (Sigma–Aldrich, St. Louis, MO, USA).…”

Section: Methodsmentioning

confidence: 99%

“…The reticulocyte counts in whole blood and RBC units drop progressively but do not disappear completely during standard blood bank storage5, 9 and reticulocytes can be detected in the recipients’ circulation up to 24–48 h post-transfusion 10 . A previous study characterized the pattern of total reticulocytes and reticulocyte subtypes in RBC units stored in additive solution-1 (AS-1: Adsol ® ) under blood bank conditions for up to six weeks, suggesting that they continue to mature slowly during refrigerated storage 9 . Blood bank storage implies not only a low temperature (4 °C) but also other conditions called the ‘storage lesion’, including but not limited to extracellular fluid acidification.…”

Section: Introductionmentioning

confidence: 99%

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In vitro kinetics of reticulocyte subtypes: maturation after red blood cell storage in additive solution-1 (AS-1)

Urbina

Palomino

2018

Hematology, Transfusion and Cell Therapy

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BackgroundReticulocytes are immature red blood cells containing RNA remnants. Their population kinetics has been documented under various in vivo and in vitro conditions, including after storage of red blood cells in blood banks. The purpose of this study was to describe the influence of blood bank storage on the kinetics of reticulocyte disappearance by in vitro culturing.MethodSamples of reticulocyte-enriched fractions (Percoll density-gradient) were obtained over different storage times from six red blood cell units stored in additive solution-1 (AS-1). Reticulocyte fractions were then cultured in enriched media at 37 °C and analyzed by flow cytometry with thiazole orange taking into account hemolysis.ResultsDensity-gradient enriched reticulocyte fractions were obtain from standard red blood cell units with <1% of reticulocytes. An exponential drop of reticulocytes was observed in cultures. The time taken for reticulocyte disappearance in cultures was shorter with increased blood bank storage time (144 ± 46 h at 0.5 weeks of storage and 15 ± 14 h in the sixth week). High fluorescence reticulocytes disappeared completely in 42.5 ± 8.5 h, medium fluorescence reticulocytes in 73.4 ± 20.8 h and low fluorescence reticulocytes in 269.9 ± 98.8 h in red blood cell units stored for half a week. These times significantly decreased in red blood cell units stored for more time.ConclusionIn vitro reticulocyte disappearance was significantly faster after prolonged storage of red blood cell units at 4 °C. The in vitro half-life at 0.5 weeks of storage was not significantly different from the values reported for fresh venous blood, but after the sixth week of storage, the half-lives were shorter. The possible explanation is that blood bank storage does not cause irreversible damage to the human reticulocyte maturational machinery.

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Section: Discussionmentioning

confidence: 93%