Reticuloendotheliosis and 'pullet disease' in Israel

Meroz, M.

doi:10.1136/vr.130.5.107-a

Cited by 6 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is noteworthy to state that several previous studies across the world detected REV sequences integrated in the genomes of field and vaccine strains of FWPV, PGPV, and TKPV [16,17,[31][32][33][34][35][36][37][38][39][40][41][42][43]. Still, no REV full or partial LTRs have never seen integrated in canarypox viruses (CNPVs) [16,17,[31][32][33][34][35][36][37].…”

Section: Introductionmentioning

confidence: 99%

“…It has been reported that the FWPV vaccines are the cause of REV provirus retention in fowls, but some researchers disagree with this hypothesis, since REV integration was detected in the FWPV genome before the wide spread of the FWPV vaccine in poultry [ 30 ]. It is noteworthy to state that several previous studies across the world detected REV sequences integrated in the genomes of field and vaccine strains of FWPV, PGPV, and TKPV [ 16 , 17 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 ]. Still, no REV full or partial LTRs have never seen integrated in canarypox viruses (CNPVs) [ 16 , 17 , 31 , 32 , 33 , 34 , 35 , 36 , 37 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular Detection of Reticuloendotheliosis Virus 5′ Long Terminal Repeat Integration in the Genome of Avipoxvirus Field Strains from Different Avian Species in Egypt

Mosad

El‐Tholoth

El-Kenawy

et al. 2020

Biology

View full text Add to dashboard Cite

Avipoxviruses (APVs) are among the most complex viruses that infect a wide range of birds’ species. The infection by APVs is often associated with breathing and swallowing difficulties, reduced growth, decreased egg production, and high mortalities in domestic poultry. In the present study, 200 cutaneous nodular samples were collected from different avian species (chicken, pigeon, turkey, and canary) suspected to be infected with APVs from Dakahlia Governorate, Egypt. Pooled samples (n = 40) were prepared and inoculated in embryonated chicken eggs (ECEs). APVs were then identified by polymerase chain reaction (PCR) and sequence analysis of the APV P4b gene. Furthermore, the forty strains of APVs were screened for the presence of reticuloendotheliosis virus (REV)-5’LTR in their genomes. Interestingly, the phylogenic tree of the APV P4b gene was separated into 2 clades: clade 1, in which our fowlpox virus (FWPV), turkeypox virus (TKPV), and canarypox virus (CNPV) isolates were grouped, along with reference FWPVs and TKPVs retrieved from GenBank, whereas, in clade2, the pigeonpox virus (PGPV) isolate was grouped with PGPVs retrieved from GenBank. Likewise, REV-5’LTR was amplified from 30 strains isolated from chicken, turkey, and canary, while PGPV strains were free from REV-5’LTR integration. To the best of our knowledge, this study involved the detection and characterization of REV-5’LTR insertions in the APVs field isolates in Egypt for the first time. Given the above information, further future research seems recommended to understand the impact of the resulting REV-5’LTR insertions on the pathogenesis, virulence, and inadequate vaccine protection against APVs.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular Detection of Reticuloendotheliosis Virus 5′ Long Terminal Repeat Integration in the Genome of Avipoxvirus Field Strains from Different Avian Species in Egypt

Mosad

El‐Tholoth

El-Kenawy

et al. 2020

Biology

View full text Add to dashboard Cite

show abstract

“…In Middle East and Africa, a REV associated lymphoma not related to contaminated vaccine have been reported (Meroz, 1992 andOkoye et al, 1993). On the other hand, in United States Fadly et al (1996) reported an outbreak of lymphomas in two broiler breeder flocks following the using of REV contaminated fowl pox vaccine.…”

Section: Introductionmentioning

confidence: 99%

Detection of Reticuloendotheliosis Virus as Contaminant of Fowl Pox Vaccines

Hamid

Awad

Abou-Rawash

et al. 2008

Kafrelsheikh Veterinary Medical Journal

View full text Add to dashboard Cite

This study was performed to detect reticuloendotheliosis virus (REV) as a contaminant in fowl pox vaccines. A total of 30 fowl pox vaccine samples for the presences of REV using the in vitro a total and in vivo methods. In the in vitro test, the fowl pox vaccine samples were inoculated into chicken embryo fibroblast (CEF) cultures prepared from SPF embryonated chichen egg and examined by PCR test for detection of REV. In the in vivo test, each fowl pox vaccine sample was inoculated in five days old SPF chicks and kept under observation up to 12 weeks post inoculation (PI); serum samples were collected on 15th , 30th and 45th day PI for the detection of antibodies against REV by commercial ELIZA kit, and tissue specimens were collected at 8th and 12th weeks PI for histopathological examination. The results revealed that: only one imported vaccine sample gave positive results by PCR test a product of 291-bp was obtained by the vaccine sample.

show abstract