Retinal Microvasculature-on-a-Chip for Modeling VEGF-Induced Permeability

Ragelle, Héloïse; Dernick, K.; Westenskow, Peter D.; Kustermann, Stefan

doi:10.1007/978-1-0716-2217-9_18

Cited by 1 publication

(2 citation statements)

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“…These consists of EC monolayer coated microfluidic channels, forming a tube or vessel-like structure adjacent to a gel-based mixture of different cell types [250,351]. Such a system using human retinal microvascular cells has been developed to assess permeability responses using leakage mediators in a retinal microvasculature-on-a chip model [352]. Although these models are useful to study barrier permeability or TJ protein expression, but they still cannot fully epitomize the in vivo permeability or morphology, such as vessel diameter and branching [351].…”

Section: Three-dimensional (3d) In Vitro Modelsmentioning

confidence: 99%

“…Labor-intensive; high heterogeneity between cell lines. [345,346,352,356,358] Abbreviations: FITC, fluorescein isothiocyanate; NIR, near infra-red; OCT, optical coherence tomography; EDI-OCT, enhanced depth imaging OCT; SS-OCT, swept source OCT; OCTA, OCT angiography; TEER, transendothelial electrical resistance; TJ, tight junction; 2-D, two dimensional; 3-D, three dimensional.…”

Section: -D Models Teer or Tracersmentioning

confidence: 99%

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Assessment of Inner Blood–Retinal Barrier: Animal Models and Methods

Bora,

Kushwah,

Maurya

et al. 2023

Cells

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Proper functioning of the neural retina relies on the unique retinal environment regulated by the blood–retinal barrier (BRB), which restricts the passage of solutes, fluids, and toxic substances. BRB impairment occurs in many retinal vascular diseases and the breakdown of BRB significantly contributes to disease pathology. Understanding the different molecular constituents and signaling pathways involved in BRB development and maintenance is therefore crucial in developing treatment modalities. This review summarizes the major molecular signaling pathways involved in inner BRB (iBRB) formation and maintenance, and representative animal models of eye diseases with retinal vascular leakage. Studies on Wnt/β-catenin signaling are highlighted, which is critical for retinal and brain vascular angiogenesis and barriergenesis. Moreover, multiple in vivo and in vitro methods for the detection and analysis of vascular leakage are described, along with their advantages and limitations. These pre-clinical animal models and methods for assessing iBRB provide valuable experimental tools in delineating the molecular mechanisms of retinal vascular diseases and evaluating therapeutic drugs.

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