Retinal receptor types inAglais urticae andPieris brassicae (Lepidoptera), revealed by analysis of the electroretinogram obtained with Fourier interferometric stimulation (FIS)

Steiner, Andreas; Paul, Rüdiger J.; Gemperlein, R.

doi:10.1007/bf00609730

Cited by 35 publications

(13 citation statements)

References 20 publications

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“…Photostable pigments absorbing in the major part of the visible range and transmittant in the red have been demonstrated to exist in photoreceptors of papilionids (Arikawa and Stavenga 1997;Arikawa et al 1999b), pierids (Ribi 1978a(Ribi , 1979bQiu et al 2002) and also in hymenopterans, Sphex (Ribi 1978b). In these cases the red filter pigments function to create red receptors: in papilionids (Arikawa and Uchiyama 1996;Arikawa et al 1999b), pierids (Ribi 1978a;Steiner et al 1987;Scherer and Kolb 1987), and Sphex: (Ribi 1978c). The ommatidia of other butterfly species reflecting in the red can hence be hypothesized to harbour red transmittant filters as well, resulting in photoreceptors with sensitivity spectra peaking in the red (Stavenga 2002).…”

Section: Coloured Screening Pigments Can Have a Display Functionmentioning

confidence: 99%

Colour in the eyes of insects

Stavenga

2002

Journal of Comparative Physiology A: Sensory, Neural, and Behav

109

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Many insect species have darkly coloured eyes, but distinct colours or patterns are frequently featured. A number of exemplary cases of flies and butterflies are discussed to illustrate our present knowledge of the physical basis of eye colours, their functional background, and the implications for insect colour vision. The screening pigments in the pigment cells commonly determine the eye colour. The red screening pigments of fly eyes and the dorsal eye regions of dragonflies allow stray light to photochemically restore photoconverted visual pigments. A similar role is played by yellow pigment granules inside the photoreceptor cells which function as a light-controlling pupil. Most insect eyes contain black screening pigments which prevent stray light to produce background noise in the photoreceptors. The eyes of tabanid flies are marked by strong metallic colours, due to multilayers in the corneal facet lenses. The corneal multilayers in the gold-green eyes of the deer fly Chrysops relictus reduce the lens transmission in the orange-green, thus narrowing the sensitivity spectrum of photoreceptors having a green absorbing rhodopsin. The tapetum in the eyes of butterflies probably enhances the spectral sensitivity of proximal long-wavelength photoreceptors. Pigment granules lining the rhabdom fine-tune the sensitivity spectra.

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Section: Coloured Screening Pigments Can Have a Display Functionmentioning

confidence: 99%

Colour in the eyes of insects

Stavenga

2002

Journal of Comparative Physiology A: Sensory, Neural, and Behav

109

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“…The sensitivity of the method can be enhanced and cell damage due to laser excitation in confocal microscopy can be minimized by the three- photon excitation of NAD(P)H in metabolic studies [replacing phototoxic UVA irradiation by the much better tolerated infra-red ]. The deconvolution of complex fluorescence emission spectra in terms of fluorophore components will be helped by new interferometric methods for the recording of excitation-emission spectra relying on Fourier analysis (Gemperlein 1978;Hirschberg and Kohen 1996;Hirschberg et al, 1997;Malik et al, 1996;Schrock et al, 1886;Soenksen et al, 1996;Steiner, 1987). The methods outlined illustrate a new approach in investigating cellular pathology.…”

Section: Discussionmentioning

confidence: 97%

“…for Microspectrofluorometry For improved excitation fluorescence spectroscopy in conjunction with microspectrofluorometry, Hirschberg and colleagues (Hirschberg and Kohen, 1996;Hirschberg et al, 1997) have developed a method of interferometric measurement (Connes, 1961;Felgett, 1958;Gemperlein, 1978;Jacquinot, 1954;Malik et al, 1996;Schrock et al, 1996;Soenksen et al, 1996;Steiner, 1987) allowing simultaneous excitation by radiation from the whole absorption band of the fluorochrome to be studied, which can potentially be integrated into the present instrument. A Michelson interferometer with one of its mirrors movable has been used to perform the wavelength coding (Gemperlein, 1978;Hirschberg and Kohen, 1996;Hirschberg et al, 1997;Steiner, 1987).…”

Section: Optional Addition To the Instrumental Setupmentioning

confidence: 98%

“…A Michelson interferometer with one of its mirrors movable has been used to perform the wavelength coding (Gemperlein, 1978;Hirschberg and Kohen, 1996;Hirschberg et al, 1997;Steiner, 1987). The interferometer produces sinusoidal variations as the difference between the two optical pathways in the interferometer, x, is varied (Hirschberg and Kohen, 1996;Hirschberg et al, 1997).…”

Section: Optional Addition To the Instrumental Setupmentioning

confidence: 99%

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Microspectrofluorometry and fluorescence imaging in the study of human cytopathology

Kohen

Gatt²,

Schachtschabel

et al. 2000

Microsc. Res. Tech.

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The study of energy pools and dynamics of specific pathways in living cells by microspectrofluorometry and fluorescence imaging produces spectral and topographic images characterizing structural and functional changes associated with cytopathology. Microspectro‐fluorometry and fluorescence imaging have been applied, together with organelle morphometry to a number of cells mimicking certain cytopathologies, including melanoma cells, long‐term malignant cells, and gene‐defective cells. These investigations of cellular pathology indicate that there is a convergence of various physiopathological processes. Cellular states that have similarities include senescence, detoxification, and transformation. While the NAD(P)H metabolic transients have been studied before, our emphasis in this article is on very rapidly scanned fluorescence images related to organelle integration and photoinduced cellular senescence. Microsc. Res. Tech. 51:469–480, 2000. © 2000 Wiley‐Liss, Inc.

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“…The computerized Fourier interferometric method of Gemperlein and colleagues (18)(19)(20)(21)(22)(23) appears to be the only application of the linear response method. In the interferometric method, schematized in Figure 2E, a continuously scanning Michelson interferometer generates stimulus and response interferograms in a range of wavelengths which are frequency coded.…”

Section: Linear-response Methodsmentioning

confidence: 99%

Electrophysiological measurements of spectral sensitivities: a review

DeVoe

de-Souza²,

Ventura³

1997

Braz J Med Biol Res

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Spectral sensitivities of visual systems are specified as the reciprocals of the intensities of light (quantum fluxes) needed at each wavelength to elicit the same criterion amplitude of responses. This review primarily considers the methods that have been developed for electrophysiological determinations of criterion amplitudes of slow-wave responses from single retinal cells. Traditional flash methods can require tedious dark adaptations and may yield erroneous spectral sensitivity curves which are not seen in such modifications as ramp methods. Linear response methods involve interferometry, while constant response methods involve manual or automatic adjustments of continuous illumination to keep response amplitudes constant during spectral scans. In DC or AC computerized constant response methods, feedback to determine intensities at each wavelength is derived from the response amplitudes themselves. Although all but traditional flash methods have greater or lesser abilities to provide on-line determinations of spectral sensitivities, computerized constant response methods are the most satisfactory due to flexibility, speed and maintenance of a constant adaptation level. Correspondence

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Retinal receptor types inAglais urticae andPieris brassicae (Lepidoptera), revealed by analysis of the electroretinogram obtained with Fourier interferometric stimulation (FIS)

Cited by 35 publications

References 20 publications

Colour in the eyes of insects

Colour in the eyes of insects

Microspectrofluorometry and fluorescence imaging in the study of human cytopathology

Electrophysiological measurements of spectral sensitivities: a review

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