Retinoic acid-loaded solid lipid nanoparticles surrounded by chitosan film support diabetic wound healing in in vivo study

Arantes, Valquíria T.; Faraco, André Augusto Gomes; Ferreira, Frederico B.; Oliveira, Cleida A.; Martins-Santos, Elisângela; Cassini‐Vieira, Puebla; Barcelos, Lucíola da Silva; Ferreira, Lucas Antônio Miranda; Goulart, Gisele Assis Castro

doi:10.1016/j.colsurfb.2019.110749

Cited by 70 publications

(33 citation statements)

References 58 publications

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“…In fact, to our knowledge, the available literature on SLN administration in vivo is based on nanoparticles loaded with active molecules and/or modified to target specific organs/tissues. In addition, no data on the SLN biodistribution in the whole organism are available but only studies on the short-term accumulation in the target organs have been performed (eg, [36][37][38][39][40]. In this view, our study represents a novel contribution to the evaluation of biocompatibility of blank SLN by i) providing information on their in vivo biodistribution after systemic (ip) administration in healthy mice up to 4 h (a time sufficient for the complete elimination of lipid nanoparticles from the organism); ii) evaluating ex vivo the accumulation of SLN in multiple organs; iii) analyzing the effects on histological and cytological features of organs that proved to accumulate nanoparticles.…”

Section: Discussionmentioning

confidence: 99%

<p>A Correlative Imaging Study of in vivo and ex vivo Biodistribution of Solid Lipid Nanoparticles</p>

Mannucci¹,

Boschi²,

Cisterna³

et al. 2020

IJN

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Solid lipid nanoparticles are largely used in biomedical research and are characterized by high stability and biocompatibility and are also able to improve the stability of various loaded molecules. In vitro studies demonstrated that these nanoparticles are low cytotoxic, while in vivo studies proved their efficiency as nanocarriers for molecules characterized by a low bioavailability. However, to our knowledge, no data on the systemic biodistribution and organ accumulation of solid lipid nanoparticles in itself are presently available. Methods: In this view, we investigated the solid lipid nanoparticles biodistribution by a multimodal imaging approach correlating in vivo and ex vivo analyses. We loaded solid lipid nanoparticles with two different fluorophores (cardiogreen and rhodamine) to observe them with an optical imager in the whole organism and in the excised organs, and with fluorescence microscopy in tissue sections. Light and transmission electron microscopy analyses were also performed to evaluate possible structural modification or damage due to nanoparticle administration. Results: Solid lipid nanoparticles loaded with the two fluorochromes showed good optic characteristics and stable polydispersity. After in vivo administration, they were clearly detectable in the organism. Four hours after the injection, the fluorescent signal occurred in anatomical districts corresponding to the liver and this was confirmed by the ex vivo acquisitions of excised organs. Brightfield, fluorescence and transmission electron microscopy confirmed solid lipid nanoparticles accumulation in hepatocytes without structural damage. Conclusion: Our results support the systemic biocompatibility of solid lipid nanoparticles and demonstrate their detailed biodistribution from the whole organism to organs until the cells.

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Section: Discussionmentioning

confidence: 99%

<p>A Correlative Imaging Study of in vivo and ex vivo Biodistribution of Solid Lipid Nanoparticles</p>

Mannucci¹,

Boschi²,

Cisterna³

et al. 2020

IJN

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“…The excellent mechanical properties and antibacterial activity of the films contributed to the fast diabetic wound healing process because of the presence of PVA and curcumin, respectively. The in vivo studies of retinoic acid-encapsulated solid lipid nanoparticles enclosed by chitosan films reported by Arantes and co-workers using STZinduced diabetic mice showed an increase in wound contraction after the 5th-day postsurgery when compared to the blank films, indicating that retinoic acid-encapsulated solid lipid nanoparticles enclosed by chitosan films accelerate skin wound closure in diabetes [83]. The features of lipid nanoparticles such as the nanosized structure are the main reason for the rapid wound closure.…”

Section: Colobatiu Et Al Reported Chitosan-based Films Encapsulated W...mentioning

confidence: 95%

Polymer-Based Wound Dressing Materials Loaded with Bioactive Agents: Potential Materials for the Treatment of Diabetic Wounds

et al. 2022

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Diabetic wounds are severe injuries that are common in patients that suffer from diabetes. Most of the presently employed wound dressing scaffolds are inappropriate for treating diabetic wounds. Improper treatment of diabetic wounds usually results in amputations. The shortcomings that are related to the currently used wound dressings include poor antimicrobial properties, inability to provide moisture, weak mechanical features, poor biodegradability, and biocompatibility, etc. To overcome the poor mechanical properties, polymer-based wound dressings have been designed from the combination of biopolymers (natural polymers) (e.g., chitosan, alginate, cellulose, chitin, gelatin, etc.) and synthetic polymers (e.g., poly (vinyl alcohol), poly (lactic-co-glycolic acid), polylactide, poly-glycolic acid, polyurethanes, etc.) to produce effective hybrid scaffolds for wound management. The loading of bioactive agents or drugs into polymer-based wound dressings can result in improved therapeutic outcomes such as good antibacterial or antioxidant activity when used in the treatment of diabetic wounds. Based on the outstanding performance of polymer-based wound dressings on diabetic wounds in the pre-clinical experiments, the in vivo and in vitro therapeutic results of the wound dressing materials on the diabetic wound are hereby reviewed.

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“…Restoration skin defects in diabetic wounds was crucial for minimising cases of limb amputations in the diabetic patients worldwide. (Arantes et al, 2020) designed and characterized a solid lipid nanoparticles retenoic acid incorporated chitosan solid lipid nanoparticles for enhancing the wound healing activity of skin defects in case of a diabetic mouse model. Further, the characterisation studies revealed about the size uniformity, biocompatibility and controlled release of the developed solid lipid nanoparticles.…”

Section: Retenoic Acidmentioning

confidence: 99%

“…Finally, it was identi ied that retenoic acid loaded solid lipid nanoparticles proved to be a promising approach for the treatment of skin defects in diabetic wounds. (Arantes et al, 2020).…”

Section: Retenoic Acidmentioning

confidence: 99%

Wound Healing Potential of Selected Drug molecules Obtained From Terpenoids

N¹,

Harikrishnan²

2020

ijrps

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Skin is the vital organ of the human system; however, due to its high exposure to the external environment, it was susceptible to various traumatic conditions. During the time of trauma human body played a significant role in the activation of various biological processes for enhancing the wound healing activity in case of damaged tissues. However, in case of skin tissue defects, to enhance the angiogenesis minimal scar formation, several pharmaceutical products were paving their way into the market. Now a days there was a rising worldwide utilization of several bioactive compounds obtained from terpenoids. Terpenoids are 5-carbon compound isoprene with oxygen-containing functional groups were isolated from several plants demonstrates a wide range of activities such as antioxidant, anti-inflammatory and anti-microbial activity. Furthermore, due to their excellent cytoprotective activity, terpenoids based products had played a significant role in wound healing activity. Moreover, in our study, because of their excellent wound healing capability, we reviewed about the few drug molecules such as Tocopherol, Farnesol, Betulin, Retenoic acid, Coenzyme Q10. However, now a day's terpenoids were utilized in several wound-healing formulations because of their excellent anti-inflammatory, re-vascularization and re-epithelization characteristics. Finally, this review focuses on the role of a few drug products obtained from terpenoids as an ideal topical wound healing agent in modern medicine.

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Retinoic acid-loaded solid lipid nanoparticles surrounded by chitosan film support diabetic wound healing in in vivo study

Cited by 70 publications

References 58 publications

<p>A Correlative Imaging Study of in vivo and ex vivo Biodistribution of Solid Lipid Nanoparticles</p>

<p>A Correlative Imaging Study of in vivo and ex vivo Biodistribution of Solid Lipid Nanoparticles</p>

Polymer-Based Wound Dressing Materials Loaded with Bioactive Agents: Potential Materials for the Treatment of Diabetic Wounds

Wound Healing Potential of Selected Drug molecules Obtained From Terpenoids

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