Retinoic acid prevents downregulation of RAS recision gene/lysyl oxidase early in adipocyte differentiation

Dimaculangan, Dwight; Chawla, Ajay; Boak, Andra M.; Kagan, Herbert M.; Lazar, Mitchell A.

doi:10.1046/j.1432-0436.1994.5810047.x

Cited by 37 publications

(21 citation statements)

References 47 publications

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“…Except for well-known adipocyte secreted proteins, such as complement C3, adiponectin, adipsin, osteonectin, several collagens, matrix metalloproteinase-2, metalloproteinase inhibitor 2, haptoglobin, and a housekeeping protein beta-2 microglobulin [40], our identification method confirmed five other secreted proteins that have been reported in only a very limited number of studies, such as MIF [41], protein-lysine 6-oxidase [42], extracellular superoxide dismutase [43], gelsolin, and PEDF [8]. Furthermore, five proteins were newly identified as adipocyte secreted factors that have previously been reported as being secreted by other cell systems: procollagen C-proteinase enhancer protein [44], galectin-1, cyclophilin A and C, and SF20/IL-25.…”

Section: Growth Regulation As a New Aspect Of Adipokines Revealed By supporting

confidence: 66%

Profiling of the secreted proteins during 3T3-L1 adipocyte differentiation leads to the identification of novel adipokines

Wang

Mariman

Keijer³

et al. 2004

CMLS, Cell. Mol. Life Sci.

132

170

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Adipose tissue is an endocrine organ capable of secreting a number of adipokines with a role in the regulation of adipose tissue and whole-body metabolism. We used two-dimensional gel electrophoresis combined with mass spectrometry to profile the secreted proteins from (pre)adipocytes. The culture medium of 3T3-L1 cells during adipocyte differentiation was screened, and 41 proteins that responded to blocking of secretion by 20 degrees C treatment and/or brefeldin A treatment were identified. Prohibitin, stress-70 protein, and adhesion-regulating molecule 1 are reported for the first time as secreted proteins. In addition, procollagen C-proteinase enhancer protein, galectin-1, cyclophilin A and C, and SF20/IL-25 are newly identified as adipocyte secreted factors. Secretion profiles indicated a dynamic environment including an actively remodeling extracellular matrix and several factors involved in growth regulation.

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Section: Growth Regulation As a New Aspect Of Adipokines Revealed By supporting

confidence: 66%

Profiling of the secreted proteins during 3T3-L1 adipocyte differentiation leads to the identification of novel adipokines

Wang

Mariman

Keijer³

et al. 2004

CMLS, Cell. Mol. Life Sci.

132

170

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“…This normal regulation of C/EBP␤ expression in the presence of RA stands in contrast to the regulation of PPAR␥, as shown in Fig. 3, as well as numerous other genes, including those encoding C/EBP␣, Rev-ErbA␣, lysyl oxidase, and aP2, whose regulated expression during adipocyte differentiation is completely prevented by RA (10,20,69). Since RA blocked the induction of PPAR␥ but not C/EBP␤, the primary mechanism of RA inhibition in normal adipogenesis appears to be at the level of C/EBP-mediated transcription, which would be consistent with the ability of RA to prevent both adipocyte differentiation and PPAR␥ expression in the cells expressing ectopic C/EBP␣ or -␤.…”

Section: Resultsmentioning

confidence: 94%

Retinoic Acid Blocks Adipogenesis by Inhibiting C/EBPβ-Mediated Transcription

Schwarz

Reginato

Shao

et al. 1997

Molecular and Cellular Biology

283

264

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Adipocyte differentiation is thought to involve sequential induction of the transcription factors C/EBP␤, peroxisome proliferator-activated receptor ␥ (PPAR␥), and C/EBP␣. C/EBP␣ expression is both necessary and sufficient for adipocyte differentiation. Here we report that ectopic expression of either C/EBP␣ or C/EBP␤ induces PPAR␥ expression and adipogenesis and that retinoic acid (RA) completely inhibits adipogenesis by either form of C/EBP. In studies of normal preadipocytes, RA does not prevent C/EBP␤ induction but blocks induction of PPAR␥, C/EBP␣, and adipogenesis. In transient transfection studies, liganded RA receptor (RAR) specifically blocks transcriptional activation by either C/EBP␣ or C/EBP␤. These results strongly suggest that C/EBP␣ substitutes for C/EBP␤ to induce adipocyte differentiation and that liganded RAR inhibits adipogenesis by blocking C/EBP␤-mediated induction of downstream genes.

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“…According to Brandebourg and Hu (2005), the retinoids, which are derivatives of vitamin A, have an inhibitory effect on cultured preadipocytes differentiation; therefore, it has been proposed that the influence of dietary vitamin A level on IMF is mediated by adipocyte differentiation (Dimaculangan et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

The effect of immunocastration and a diet based on granulated barley on growth performance and carcass, meat and fat quality in heavy gilts

Daza

Latorre

Olivares

et al. 2014

Animal

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A total of 48 Duroc × (Landrace × Large White) gilts of 33.2 kg BW were used to investigate the influence of immunocastration and diet on growth performance and carcass, meat and fat quality. Four treatments were arranged factorially (2 × 2) with two sexes (immunocastrated gilts: IG v. entire gilts: EG) and two dietary treatments (a commercial feedstuff as control v. granulated barley as a single major ingredient) provided during the finishing period (from 103 to 126 kg BW). There were four replicates of three pigs per treatment. At the end of the trial, the IGs grew faster ( P < 0.05) and ate more feed ( P < 0.05) than the EGs. Carcasses from the IGs had thicker backfat depth than those from the EGs ( P < 0.01) and carried out a lower percentage of rejected carcasses ( P < 0.05) at slaughterhouse owing to lack of fat. Meat from the IGs tended to have higher intramuscular fat (IMF) content in the Longissimus thoracis (LT) muscle than that from the EGs ( P = 0.09). In addition, immunocastration increased the total saturated fatty-acid proportion in subcutaneous fat and IMF ( P < 0.001) and decreased the total monounsaturated fatty acid (MUFA) and total polyunsaturated fatty acid (PUFA) percentages in subcutaneous backfat ( P < 0.05 and P < 0.001, respectively) and in IMF ( P < 0.01 and P = 0.06, respectively). The use of a diet based on granulated barley during the finishing period had no effect on growth performance but tended to increase IMF content in the LT muscle ( P < 0.06), and increased MUFA ( P < 0.05) and decreased PUFA ( P < 0.01) proportions in omental fat. It is concluded that immunocastration of gilts intended for dry-cured ham industry improved some aspects of growth performance and carcass and meat quality, whereas granulated barley had scarce effect on productive traits and fatty-acid profile but tended to improve IMF content.

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Retinoic acid prevents downregulation of RAS recision gene/lysyl oxidase early in adipocyte differentiation

Cited by 37 publications

References 47 publications

Profiling of the secreted proteins during 3T3-L1 adipocyte differentiation leads to the identification of novel adipokines

Profiling of the secreted proteins during 3T3-L1 adipocyte differentiation leads to the identification of novel adipokines

Retinoic Acid Blocks Adipogenesis by Inhibiting C/EBPβ-Mediated Transcription

The effect of immunocastration and a diet based on granulated barley on growth performance and carcass, meat and fat quality in heavy gilts

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