Retinoid-Related Orphan Receptor α Controls the Early Steps of Purkinje Cell Dendritic Differentiation

Boukhtouche, Fatiha; Janmaat, Sonja; Vodjdani, Guilan; Gautheron, Vanessa; Mallet, Jacques; Dusart, Isabelle; Mariani, Jean

doi:10.1523/jneurosci.4636-05.2006

Cited by 83 publications

(90 citation statements)

References 59 publications

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“…5A) (Kapfhammer, 2004). We first observed that, as previously described in the mouse (Boukhtouche et al, 2006), rat PCs in organotypic slices cultured at P0 are bipolar and fusiform (stage 1) during the very first days in vitro (div). This stage corresponds to the early fusiform stage described in vivo (Ramó n y Cajal, 1926;Armengol and Sotelo, 1991).…”

Section: Sclip Is Strongly Expressed In Developing Purkinje Cells In mentioning

confidence: 63%

“…Indeed, the large majority of transduced cells infected at P0 are PCs (Fig. 4A) (Boukhtouche et al, 2006). Furthermore, the neighboring untransduced PCs within the same cellular environment are not affected, and exhibit a normal morphology with protrusions at stage 2 and dendritic processes at stages 3 and 4.…”

Section: Discussionmentioning

confidence: 95%

“…Their dendritic differentiation involves early and late developmental stages characterized by dynamic regressive and growth processes that are tightly regulated (Ramó n y Cajal, 1911;Armengol and Sotelo, 1991;Boukhtouche et al, 2006). In this study, we examined the biological properties and functions of the stathmin family protein SCLIP during PC dendritic development.…”

Section: Discussionmentioning

confidence: 99%

“…We classified PC differentiation stages as described previously (Boukhtouche et al, 2006). Briefly, fusiform PCs with a bipolar shape were defined as stage 1, stellate PCs with processes all around the soma as stage 2, PCs with several long and mature perisomatic dendritic processes as stage 3, and PCs with a single dendritic tree giving rise to additional side branches as stage 4.…”

Section: Imaging Quantification Of Pc Differentiation Stages and Momentioning

confidence: 99%

“…Various factors have been implicated in the regulation of these late phases of dendritic development (Kapfhammer, 2004). However, those controlling early stages of dendritogenesis remain poorly characterized (Boukhtouche et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

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SCLIP Is Crucial for the Formation and Development of the Purkinje Cell Dendritic Arbor

Poulain¹,

Chauvin²,

Wehrlé³

et al. 2008

J. Neurosci.

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Section: Sclip Is Strongly Expressed In Developing Purkinje Cells In mentioning

confidence: 63%

Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Section: Imaging Quantification Of Pc Differentiation Stages and Momentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

SCLIP Is Crucial for the Formation and Development of the Purkinje Cell Dendritic Arbor

Poulain¹,

Chauvin²,

Wehrlé³

et al. 2008

J. Neurosci.

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Formation and maturation of parallel fiber‐purkinje cell synapses in the staggerer cerebellum ex vivo

Janmaat¹,

Frédéric²,

Sjollema³

et al. 2008

J of Comparative Neurology

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In vivo, homozygous staggerer (Rorasg/sg) Purkinje cells (PCs) remain in an early stage of development with rudimentary spineless dendrites, associated with a lack of parallel fiber (PF) input and the persistence of multiple climbing fibers (CFs). In this immunocytochemical study we used cerebellar organotypic cultures to monitor the development of Rorasg/sg PF‐PC synapses in the absence of CF innervation. Ex vivo the vesicular glutamate transporters VGluT1 and VGluT2 reactivity was preferentially localized around the Rorasg/sg PC soma and proximal dendrites, which are typically CF domains. The shift from VGluT2 to VGluT1 in PF terminals during development was delayed in Rorasg/sg slices. The postsynaptic receptors mGluR1 and GluRδ2 were differently distributed on Rorasg/sg PCs. mGluR1 reactivity was evenly distributed in PC soma and dendrites, whereas GluRδ2 reactivity, normally restricted at PF synapses, was dense in Rorasg/sg PC somata. The presynaptic distribution of VGluT1 and VGluT2 on Rorasg/sg PCs matched the postsynaptic distribution of the glutamate receptor GluRδ2, but not mGluR1. J. Comp. Neurol. 512:467–477, 2009. © 2008 Wiley‐Liss, Inc.

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Death and survival of heterozygous Lurcher Purkinje cells In vitro

Zanjani¹,

McFarland²,

Cavelier³

et al. 2009

Developmental Neurobiology

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The differentiation and survival of heterozygous Lurcher (+/Lc) Purkinje cells in vitro was examined as a model system for studying how chronic ionic stress affects neuronal differentiation and survival. The Lurcher mutation in the δ2 glutamate receptor (GluRδ2) converts an orphan receptor into a membrane channel that constitutively passes an inward cation current. In the GluRδ2+/Lc mutant, Purkinje cell dendritic differentiation is disrupted and the cells degenerate following the first week of postnatal development. To determine if the GluRδ2+/Lc Purkinje cell phenotype is recapitulated in vitro, +/+ and +/Lc Purkinje cells from postnatal day 0 pups were grown in either isolated cell or cerebellar slice cultures. GluRδ2+/+ and GluRδ2+/Lc Purkinje cells appeared to develop normally through the first 7 days in vitro (DIV), but by 11 DIV GluRδ2+/Lc Purkinje cells exhibited a significantly higher cation leak current. By 14 DIV, GluRδ2+/Lc Purkinje cell dendrites were stunted and the number of surviving GluRδ2+/Lc Purkinje cells was reduced by 75% compared to controls. However, treatment of +/Lc cerebellar cultures with 1-naphthyl acetyl spermine (NASP) increased +/Lc Purkinje cell survival to wild type levels. These results support the conclusion that the Lurcher mutation in GluRδ2 induces cell autonomous defects in differentiation and survival. The establishment of a tissue culture system for studying cell injury and death mechanisms in a relatively simple system like GluRδ2+/Lc Purkinje cells will provide a valuable model for studying how the induction of a chronic inward cation current in a single cell type affects neuronal differentiation and survival.

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Retinoid-Related Orphan Receptor α Controls the Early Steps of Purkinje Cell Dendritic Differentiation

Cited by 83 publications

References 59 publications

SCLIP Is Crucial for the Formation and Development of the Purkinje Cell Dendritic Arbor

SCLIP Is Crucial for the Formation and Development of the Purkinje Cell Dendritic Arbor

Formation and maturation of parallel fiber‐purkinje cell synapses in the staggerer cerebellum ex vivo

Death and survival of heterozygous Lurcher Purkinje cells In vitro

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