Retinoids and retinoid-binding protein expression in rat adipocytes.

Tsutsumi, Chiharu; Okuno, M; Tannous, L.; Piantedosi, Roseann; Allan, Maggi; Goodman, D S; Blaner, William S.

doi:10.1016/s0021-9258(18)46017-6

Cited by 280 publications

(48 citation statements)

References 40 publications

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“…For example, in agreement with Eriksson et al (34), we found Crbp1 expression to be highest in liver, with progressively lower levels in kidney, lung, and spleen. Although we also detected relatively high expression of Crbp1 in WAT, it is known that this mRNA is exclusively expressed in the stromovascular fraction, whereas Crbp3 is primarily expressed in adipocytes (35, 36). In this regard, we also showed relatively high levels of Crbp3 expression in WAT and BAT.…”

Section: Discussionmentioning

confidence: 43%

Chronic alcohol consumption has a biphasic effect on hepatic retinoid loss

2015

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The alcohol-induced depletion of hepatic retinoid stores correlates with the progression of liver injury; however, the mechanisms underlying alcohol's effects have not been fully elucidated. Our goal was to gain a mechanistic understanding of alcohol-induced hepatic retinoid depletion. Wild-type and mutant mice were continuously fed alcohol through Lieber-DeCarli liquid diets, with matched control animals pair fed an isocaloric alcohol-free diet to ensure equal nutrient and calorie intake between groups. A systematic analysis of tissue retinol and retinyl ester levels was performed with HPLC, complemented by gene and protein expression analyses. Our results delineated 2 phases of alcohol-induced depletion of hepatic retinoid. Initially, ∼15% of hepatic retinoid content was mobilized from the liver, causing extrahepatic tissue retinoid levels to increase. Subsequently, there was a precipitous drop in hepatic retinoid content (>60%), without further retinoid accumulation in the periphery. Follow-up studies in mutant mice revealed roles for RBP, CRBP1, and CD36 in retinoid mobilization and extrahepatic retinoid uptake, as well as a role for CYP2E1 in the catabolism of hepatic retinoid. In summary, alcohol has a biphasic effect on hepatic retinoid stores, characterized by an initial phase of rapid mobilization to extrahepatic tissues followed by extensive catabolism within the liver.

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Section: Discussionmentioning

confidence: 43%

Chronic alcohol consumption has a biphasic effect on hepatic retinoid loss

2015

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“…1 Under normal conditions, hepatocytes are considered the main source of circulating RBP4, but adipose tissue has the second highest expression level (20%-40% of levels in the liver). 2 The regions near the RBP4 locus on human chromosome 10q have been linked to an increased risk of type 2 diabetes mellitus (DM). 3,4 Recently, RBP4 with insulin-mediated suppression of glucose production in the liver.…”

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confidence: 99%

Increased Serum Retinol-Binding Protein 4 Concentrations in Women With Gestational Diabetes Mellitus

Chan

Chen

et al. 2007

Reprod. Sci.

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The authors hypothesized that serum retinol-binding protein 4 (RBP4) concentrations will be higher in gestational diabetes mellitus (GDM) subjects. This study tested both women with GDM and healthy pregnant women and correlated their serum RBP4 concentrations with body mass index (BMI) and a variety of other parameters. Also, since there is no information on the relationship between RBP4 concentrations in maternal and fetal serum, this study measured these at delivery and examined whether there were correlations between the cord serum RBP4 levels and maternal serum RBP4 concentrations, neonatal birth weights, and gestational age at delivery. A total of 40 women were evaluated: 20 women with GDM and 20 healthy pregnant women to serve as control subjects. Serum RBP4 concentrations were analyzed with the use of an enzyme-linked immunosorbent assay kit. Serum RBP4 concentrations at glucose challenge test (GCT) were significantly higher in the GDM group (42.4 +/- 13.8 ng/mL) than in the healthy control group (32.0 +/- 8.7 ng/mL; P = .007). BMI at GCT (P = .003) and GDM/no GDM (P = .014) were significantly correlated to serum RBP4 concentrations at GCT by multiple linear regression analysis. In GDM subjects, serum RBP4 concentrations immediately after delivery were significantly lower than those at GCT (30.1 +/- 11.0 ng/mL, 42.4 +/- 13.8 ng/mL; P < .001), but there was no such difference in normal subjects (30.9 +/- 10.0 ng/mL, 32.0 +/- 8.7 ng/mL; P = .581). Cord serum RBP4 concentrations were significantly lower than maternal serum RBP4 concentrations at delivery (10.9 +/- 3.8 ng/mL, 30.5 +/- 10.4 ng/mL; P < .001). Only fetal birth weight (P = .049) was independently related to cord serum RBP4 concentrations at delivery by multiple linear regression analysis. This study found increased serum RBP4 concentrations at GCT in GDM subjects, and GDM was significantly correlated to serum RBP4 levels after adjustment for the effect of BMI. Lower RBP4 concentrations were found at delivery in GDM subjects. Maternal serum RBP4 concentrations were significantly higher than cord serum RBP4 concentrations, and fetal birth weights were independently correlated to cord serum RBP4 concentrations. These findings may indicate that RBP4 plays a role in the pathogenesis of GDM. However, further experiments are required to clarify this role and find a possible regimen for GDM treatment.

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“…In 1 animal model, RBP4 expression was much higher in the liver than in adipose tissue. 29 A recent trial found that higher RBP4 levels are associated with liver fat content rather than visceral or subcutaneous fat and, consequently, liver IR. 30 In both adults and children, a gender difference in serum RBP4 concentration has been observed, with levels being lower in female than in male participants.…”

Section: Discussionmentioning

confidence: 99%