2019
DOI: 10.1177/1535370219838785
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Retinol isotope dilution accurately predicts liver reserves in piglets but overestimates reserves in lactating sows

Abstract: Retinol isotope dilution (RID) is used to estimate total body vitamin A (VA) stores in groups to assess VA status. Metabolic differences during lactation may affect RID calculations as currently applied. We evaluated the time required for isotopic equilibration between serum and liver retinol in piglets, and the utility of milk retinol isotopic enrichment as a proxy for serum in lactating sows. Piglets ( n = 24) and sows ( n = 6) were fed 1.75 or 20 µmol 13 C2-retinyl acetate, respectively. Piglets ( n = 5 or… Show more

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Cited by 8 publications
(3 citation statements)
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“…Labeled VA ([ 13 C]-retinol) given to piglets followed a similar serum:liver distribution pattern seen in studies of older humans or animals, first appearing in the serum at 1–2 d following dosing, remaining higher in serum than liver at day 4, decreasing at day 7, and trending lower at 14 d with a 0.91 serum:liver enrichment ratio, indicating the dose mixed with the body pool and had lower enrichment in serum than the liver due to a small amount of VA coming through the diet ( 92 ).…”
Section: Resultsmentioning
confidence: 99%
“…Labeled VA ([ 13 C]-retinol) given to piglets followed a similar serum:liver distribution pattern seen in studies of older humans or animals, first appearing in the serum at 1–2 d following dosing, remaining higher in serum than liver at day 4, decreasing at day 7, and trending lower at 14 d with a 0.91 serum:liver enrichment ratio, indicating the dose mixed with the body pool and had lower enrichment in serum than the liver due to a small amount of VA coming through the diet ( 92 ).…”
Section: Resultsmentioning
confidence: 99%
“…A correction factor was not needed in VA-deficient piglets between 7 and 14 days. 16 While a seven-day draw could assess the TLR during VA program evaluation, the underlying VA status might affect the early calculations because of incomplete mixing of the dose with the body pool. Modification of the method to evaluate RID in blood samples seven days after dosing has the potential to quantitatively assess TBS in a shorter timeframe and because of less catabolic loss of the labeled retinol, it may reduce sample volumes needed for analysis on some mass spectrometers.…”
Section: Discussionmentioning
confidence: 99%
“…In VA-deficient piglets, mean TLR calculations at 7 and 14 days after isotope administration did not differ. 16 Thus, we tested whether a shorter sampling period (seven days) could be used in children, which might be more practical in field settings.…”
Section: Introductionmentioning
confidence: 99%