Retos diagnósticos de la gripe

Leonardo, Raúl Ortiz de Lejarazu; Rello, Silvia Rojo; Sanz-Muñoz, Iván

doi:10.1016/s0213-005x(19)30182-x

Cited by 3 publications

(1 citation statement)

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“…This process is time-consuming and laborious. The use of viral culture, an essential tool in the epidemiological surveillance and diagnosis of influenza viruses, has been relegated by the use of more sensitive and affordable molecular techniques [ 13 ]. For example, in molecular assays, especially in multiplex formats, a multiplex reverse transcriptase polymerase chain reaction (conventional RT–PCR) was developed to simultaneously differentiate the avian H5, H7 and H9 subtypes [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Differential Detection of H5, H7, H9 and Nine NA Subtypes of Avian Influenza Viruses via a GeXP Assay

Luo,

Xie,

et al. 2024

Microorganisms

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H5, H7 and H9 are the most important subtypes of avian influenza viruses (AIVs), and nine neuraminidase (NA) subtypes (N1–N9) of AIVs have been identified in poultry. A method that can simultaneously detect H5, H7, H9 and the nine NA subtypes of AIVs would save time and effort. In this study, 13 pairs of primers, including 12 pairs of subtype-specific primers for detecting particular subtypes (H5, H7, H9 and N1–N9) and one pair of universal primers for detecting all subtypes of AIVs, were designed and screened. The 13 pairs of primers were mixed in the same reaction, and the 13 target genes were simultaneously detected. A GeXP assay using all 13 pairs of primers to simultaneously detect H5, H7, H9 and the nine NA subtypes of AIVs was developed. The GeXP assay showed specific binding to the corresponding target genes for singlet and multiplex templates, and no cross-reactivity was observed between AIV subtypes and other related avian pathogens. Detection was observed even when only 102 copies of the 13 target genes were present. This study provides a high-throughput, rapid and labor-saving GeXP assay for the simultaneous rapid identification of three HA subtypes (H5, H7 and N9) and nine NA subtypes (N1–N9) of AIVs.

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Section: Introductionmentioning

confidence: 99%