RETRACTED: AEG-1/miR-221 Axis Cooperatively Regulates the Progression of Hepatocellular Carcinoma by Targeting PTEN/PI3K/AKT Signaling Pathway

Kannan, M.D.; Jayamohan, Sridharan; Moorthy, Rajesh Kannan; Chabattula, Siva Chander; Mathan, Ganeshan; Arockiam, Antony Joseph Velanganni

doi:10.3390/ijms20225526

Cited by 20 publications

(24 citation statements)

References 60 publications

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“…Similarly, our studies revealed that miR‐23a‐3p suppresses U251 cell growth and metastasis by targeting PTEN. In addition, several studies have shown that PTEN is negatively correlated with the high invasiveness of cancer cells (Salvatore et al, 2019), for example, upregulation of PTEN was found to suppress cell migration and invasion by blocking the PI3K/Akt pathway in non‐small cell lung cancer (Liao et al, 2019) and hepatocellular carcinoma (Kannan et al, 2019), and inactivation of MEK1/ERK1 in breast cancer (Liang et al, 2019). The above results suggest that the overexpression of PTEN may be an excellent clinical treatment method for glioma.…”

Section: Discussionmentioning

confidence: 99%

RETRACTED:CircFBXW7alleviates glioma progression through regulatingmiR‐23a‐3p/PTENaxis

Gao¹,

Yang²,

Huang³

et al. 2020

The Anatomical Record

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Increasing evidence has confirmed that circular RNAs (circRNAs) are involved in regulating the development and progression of various tumors. The aim of this study was to examine the effect of circFBXW7 on the progression of glioma and to determine its underlying mechanism. qRT‐PCR was performed to measure the expression of circFBXW7, miR‐23a‐3p, and PTEN in tissues and cell lines of glioma. The proliferation ability of glioma cells was examined using the CCK‐8 assay. Glioma cell migration and invasion capacity were detected using Transwell assays. The dual‐luciferase reporter gene assay was employed to examine the correlation between miR‐23a‐3p and circFBXW7 or PTEN. The expression levels of the related genes were determined using western blotting analysis. A glioma xenograft tumor model was employed to evaluate the functional roles of circFBXW7 in vivo. CircFBXW7 was found to be aberrantly downregulated in glioma tumor tissues and cell lines. Overexpression of circFBXW7 was found to significantly inhibit the proliferation, migration and invasion ability of the glioma cells. Moreover, bioinformatic analysis and dual‐luciferase reporter assays confirmed that circFBXW7 can directly target miR‐23a‐3p, which then blocks the binding of miR‐23a‐3p to the 3′ un‐translated region (UTR) of PTEN. Mechanically, circFBXW7 suppresses cell proliferation and metastasis in glioma by sponging miR‐23a‐3p, resulting in elevated PTEN expression. In addition, in vivo experiments also confirmed that circFBXW7 overexpression effectively halts tumor growth and metastasis. Consistent with the in vitro observations, circFBXW7 overexpression significantly decreased miR‐23a‐3p, Ki‐67, and N‐cadherin, as well as increased PTEN and E‐cadherin levels. Our results revealed that circFBXW7 exhibits antiproliferative and antimetastasis activities via sponging miR‐23a‐3p to elevate PTEN expression in glioma, which may offer a novel target for clinical therapy and diagnosis of glioma.

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Section: Discussionmentioning

confidence: 99%

RETRACTED:CircFBXW7alleviates glioma progression through regulatingmiR‐23a‐3p/PTENaxis

Gao¹,

Yang²,

Huang³

et al. 2020

The Anatomical Record

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“…Despite developments in liver cancer treatment, with improved surgical techniques and interventional therapies, the 5-year survival rate remains poor and the mortality rate has increased significantly over the past 20 years ( 2 , 3 ). Unlike other organs, the liver displays some potential to regenerate, but the proliferation rate of liver cancer cells is higher compared with that of normal liver cells due to several deregulated signaling pathways during cancer, such as PTEN, PI3K and AKT ( 4 ). Such deregulation occurs throughout all stages of tumor development, which leads to inconsistent and ineffective diagnosis and treatment of liver cancer ( 5 ).…”

Section: Introductionmentioning

confidence: 99%

Low HOXC10 expression in liver cancer regulates proliferation via a mechanism involving miR‑221 and the MAPK signaling pathway

Zhao

Guo

et al. 2020

Oncol Lett

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Homeodomain-containing gene 10 (HOXC10) is associated with the progression of a variety of different types of human cancer; however, the role of HOXC10 in liver cancer is not completely understood. The present study aimed to investigate the mechanisms underlying the effects of HOXC10 on liver cancer tumorigenesis. Quantitative PCR and western blotting were used to detect the expression patterns of HOXC10 in cancer and adjacent healthy tissues. EdU, Cell Counting Kit-8 and colony formation assays were used to determine the functions of HOXC10 in liver cancer cell lines. ENCORI, TargetScan and miRTarBase were used to identify microRNAs that target HOXC10. The verification of the interaction between HOXC10 and microRNA-221 was determined by a luciferase assay. Compared with adjacent non-cancerous tissues, the expression of HOXC10 was markedly decreased in liver cancer tissues. A HOXC10 small interfering (si)RNA significantly attenuated HOXC10 expression at the mRNA and protein levels, and enhanced cell proliferation compared with the siRNA-negative control group. In addition, the luciferase reporter assay indicated that microRNA-221 directly bound to the 3'-untranslated region of HOXC10, and interfered with the inhibitory effect of HOXC10 on proliferation. In addition, HOXC10 knockdown elevated the expression levels of mitogen-activated protein kinase signaling pathway markers compared with the siRNA-negative control group. Therefore, the results of the present study may aid with the development of novel therapeutic regimens and diagnostic markers of liver cancer.

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“…Emerging evidence has con rmed low expression of miR-125b in human breast cancer clinical biopsy samples and cell lines [22], whereas miR-125b has been shown to be highly expressed in prostate cancer clinical tissue samples [23]. Although previous studies have reported that miR-221 can act as an oncogene and is involved in the regulation of various cellular processes, including cell differentiation, apoptosis, proliferation, survival, and metastasis in different types of cancers [24,25], in this study, miR-221 may act as a tumor suppressor in NPC.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-221 Targets FBXW11 to Inhibit NPC Cell Proliferation by Regulating PTEN Signaling

Song

Zeng

et al. 2021

Preprint

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Background: In the current study, we aim to demonstrate the biological function and molecular regulatory mechanisms of miR-221 in human nasopharyngeal carcinoma (NPC). Methods: The quantitative real time-polymerase chain reaction was used to measure the expression of miR-221 in NPC clinical tissues and cells. And the flow cytometry assay was used to demonstrate the role of miR-221 on cell cycle, and the potential target of miR-221 was predicted and identified using luciferase reporter assay.Results: Our results demonstrate that miR-221 expression was significantly decreased in NPC tissues and cell lines. We also confirmed that inhibition of miR-221 could induce G1/S cell cycle transition through upregulation of the cyclin D-CDK4/6 complex but not cyclin E-CDK2 complexes. Furthermore, luciferase reporter assay demonstrated that miR-221 could directly bind to the 3’-UTR of FBXW11. FBXW11 expression was found to increase in NPC, and was inversely correlated with miR-221 expression; thus, FBXW11 expression interfered in the biological function of miR-221. We further confirmed that miR-221 targeted FBXW11 to inhibit proliferation and promote apoptosis in NPC cell lines through regulating the PTEN signaling pathway. Conclusion: our findings suggest that miR-221 plays an important role as a tumor suppressive factor in the occurrence and progression of NPC.

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RETRACTED: AEG-1/miR-221 Axis Cooperatively Regulates the Progression of Hepatocellular Carcinoma by Targeting PTEN/PI3K/AKT Signaling Pathway

Cited by 20 publications

References 60 publications

RETRACTED:CircFBXW7alleviates glioma progression through regulatingmiR‐23a‐3p/PTENaxis

RETRACTED:CircFBXW7alleviates glioma progression through regulatingmiR‐23a‐3p/PTENaxis

Low HOXC10 expression in liver cancer regulates proliferation via a mechanism involving miR‑221 and the MAPK signaling pathway

MicroRNA-221 Targets FBXW11 to Inhibit NPC Cell Proliferation by Regulating PTEN Signaling

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