RETRACTED: An alkali-thermostable xylanase from Bacillus pumilus functionally expressed in Kluyveromyces lactis and evaluation of its deinking efficiency

Thomas, Lyn C.; Ushasree, Mrudula Vasudevan; Pandey, Ashok

doi:10.1016/j.biortech.2014.03.037

Cited by 40 publications

(20 citation statements)

References 16 publications

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“…Among bacteria, Bacillus species have been reported widely as the most potent xylanolytic enzyme producers such as Bacillus sp., B. halodurans (Gupta et al 2015), B. pumilus (Thomas et al 2014), B. subtilis (Banka et al 2014), B. amyloliquefaciens, B. circulans, andB. stearothermophilus (Chakdar et al 2016).…”

Section: Bacterial Sources Of Xylanasementioning

confidence: 99%

“…MSY-2 and Flavobacterium frigidarium (Humphry et al 2001;Dornez et al 2011) Pseudoalteromonas haloplanktis TAH3A (Van Petegem et al 2002). Several alkali stable xylanases have been isolated from firmicutes such as B. pumilus (Thomas et al 2014), B. halodurans TSEV1 (Kumar and Satyanarayana 2014) and Geobacillus thermoleovorans (Verma and Satyanarayana 2012b) and actinomycetes such as Actinomadura sp. Cpt20 (Taibi et al 2012) and Streptomyces althioticus LMZM (Luo et al 2016).…”

Section: Bacterial Sources Of Xylanasementioning

confidence: 99%

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A detailed overview of xylanases: an emerging biomolecule for current and future prospective

Bhardwaj

Kumar

Verma

2019

Bioresour. Bioprocess.

299

105

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Xylan is the second most abundant naturally occurring renewable polysaccharide available on earth. It is a complex heteropolysaccharide consisting of different monosaccharides such as l-arabinose, d-galactose, d-mannoses and organic acids such as acetic acid, ferulic acid, glucuronic acid interwoven together with help of glycosidic and ester bonds. The breakdown of xylan is restricted due to its heterogeneous nature and it can be overcome by xylanases which are capable of cleaving the heterogeneous β-1,4-glycoside linkage. Xylanases are abundantly present in nature (e.g., molluscs, insects and microorganisms) and several microorganisms such as bacteria, fungi, yeast, and algae are used extensively for its production. Microbial xylanases show varying substrate specificities and biochemical properties which makes it suitable for various applications in industrial and biotechnological sectors. The suitability of xylanases for its application in food and feed, paper and pulp, textile, pharmaceuticals, and lignocellulosic biorefinery has led to an increase in demand of xylanases globally. The present review gives an insight of using microbial xylanases as an "Emerging Green Tool" along with its current status and future prospective.

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Section: Bacterial Sources Of Xylanasementioning

confidence: 99%

Section: Bacterial Sources Of Xylanasementioning

confidence: 99%

A detailed overview of xylanases: an emerging biomolecule for current and future prospective

Bhardwaj

Kumar

Verma

2019

Bioresour. Bioprocess.

299

105

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“…Bacillus sp. is a potential source of xylanases among bacteria, and a number of bacilli, such as B. stearothermophilus, B. circulans, B. licheniformis, B. pumilus, B. subtilis, B. halodurans and B. amyloliquefaciens attributed to synthesize extracellular xylanase (9, 10, 11).…”

Section: Introductionmentioning

confidence: 99%

Exploration of endo-xylanase from novel strain ofBacillus velezensisAG20 isolated from the cave of Meghalaya

Ghosh

Mahanta

Banerjee

et al. 2020

Preprint

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32Cave sets the example of extreme ecological niche and habitat for diversified 33 microorganisms. Present study involved in the isolation of endoxylanase producing novel strain 34 Bacillus velezensis AG20 from the Krem Phyllut cave, Meghalaya, India. Culture dependent 35 studies, molecular phylogentics, RNA secondary folding pattern based on 16S rDNA 36 substantiated the identity of this novel strain. Bacillus velezensis AG20 revealed the superbug 37 quality having resistance against various class of broad-spectrum antibiotics. Bacillus velezensis 38 AG20 revealed biofilm formation over the cell surface in FESEM. Highest cell biomass and 39 xylanase production supported in TB medium, further purified partially to 5.3 fold with 21% 40 yield. Molecular weight of the purified xylanase found to be 45 kDa. Enzyme kinetics and 41 pattern of hydrolysis revealed the evidence for the selection of linear birchwood xylan with Vmax 42 = 21.0 ± 3.0 U/ml, Km = 1.25 mg/ml, Kcat = 1.75/s at optimum pH 7 and temperature 50 o C also 43 found significant statistically in Taguchi's orthogonal design. Conversely, ruled out any exo-44 acting activity against synthetic pNP-xylopyranoside substrate. Endo-xylanase isolated from 45 Bacillus velezensis AG20 was moderately thermostable over temperatures 50 and 60 o C. Time 46 dependent hydrolysis of agro-waste sugar cane bagasse depicted the production of xylo-XOS hold their prebiotic potential by promoting the growth of probiotics Bifidobacterium and 49 Lactobacillus as well as high stability (~90%) against systemic fluids. Mixed XOS (300 µg/ml) 50 displayed anti-proliferation activities by reducing the growth of HT-29 and Caco-2 cells 51 significantly 90% and 75%, respectively, after 48 h. 52 53 IMPORTANCE 54 Extremophiles dwelling inside the caves have laden with the extraordinary capabilities of 55 bioconversion by nature. The pristine ecological niche inside the cave, absence of proper light 56 and air, supports the livelihood of novel microorganisms. In India, Meghalaya is hoisting longest 57 caves in the East Khasi Hills, providing conducive environment for novel bacterial strains. With the prime objective of isolating novel bacterial strains that produce extracellular xylanase our studies have been carried out. Considering the present industrial demand for nutraceutical, 60 prebiotics, anti-proliferating agents and biofuels by the conversion of lignocellulosic biomass 61 (LCB), novel enzymes are required. Xylanases from bacterial origin play a significant role in 62 conversion of LCB into oligosaccharides. Therefore, exploration and characterization of 63 xylanase producing novel isolate from cave may pave the new arena for the production of 64 prebiotic and anti-inflammatory oligosaccharides from agro-waste. 65 66 67

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“…Xylanases originating from the Bacillus strains are intrinsically interesting because of their characteristics, such as alkali and salt tolerance, cold adaptation, or thermostability; they are widely employed in industrial applications (Chang et al 2004;Huang et al 2006;Mamo et al 2006;Ruller et al 2008;Khandeparker et al 2011;Haddar et al 2012;Thomas et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Characterization of a Salt-Tolerant and Cold-Adapted Xylanase from Bacillus cellulosilyticus

Sun

Shi

et al. 2016

BioResources

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A xylanase (Xyn10A) gene from the saline-alkali-tolerant microorganism Bacillus cellulosilyticus DSM 2522 was cloned and expressed in Escherichia coli BL21 (DE3). The open reading frame was composed of 1008 base pairs, and it encoded 335 amino acid residues belonging to glycosyl hydrolase family 10. The optimal temperature and pH of the purified Xyn10A were 40 °C and 8.0, respectively. The Xyn10A was sensitive to heat and showed obvious cold-adapted activity, retaining 38.3%, 55.7%, and 82.9% of the optimal activity at 4, 20, and 30 °C, respectively. Xyn10A also showed a high level of NaCl tolerance. The highest activity was observed with 1.5 M NaCl. The specific enzyme activity of Xyn10A was as much as 163.8 U/mg. Kinetic assays showed that Km, Vmax, and Kcat were 2.56 mg/mL, 202.5 μM/min/mg, and 132.6 /s, respectively. Additionally, the main hydrolysis products using birchwood xylan as substrate were xylobiose, xylotriose, and xylotetraose, as determined by thin layer chromatography analysis. As a cold-adapted and salt-tolerant enzyme, Xyn10A is an ideal candidate for further research and biotechnological applications.

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RETRACTED: An alkali-thermostable xylanase from Bacillus pumilus functionally expressed in Kluyveromyces lactis and evaluation of its deinking efficiency

Cited by 40 publications

References 16 publications

A detailed overview of xylanases: an emerging biomolecule for current and future prospective

A detailed overview of xylanases: an emerging biomolecule for current and future prospective

Exploration of endo-xylanase from novel strain ofBacillus velezensisAG20 isolated from the cave of Meghalaya

Characterization of a Salt-Tolerant and Cold-Adapted Xylanase from Bacillus cellulosilyticus

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