The aim of this study was to evaluate the antimicrobial and antibiofilm activity of silver nanoparticles of Azadirachta indica (AISNPs) against the pathogens isolated from effluent. Green synthesis approach was opted for the synthesis of the synthesis of AISNPs by using the ethanolic extract of Azadirachta indica which was added to 1mM solution of AgNO 3 . These synthesized nanoparticles were molecularly characterized by UV-Visible spectrophotometer, TEM, Zeta Sizer, Zeta Potential, XRD and FTIR respectively. Antimicrobial activity of the AISNPs was carried by well diffusion method on Muller Hinton Agar against biofilm-producing multi-drug resistant effluent pathogens, which were identified as E. coli, P. aeruginosa, S. aureus and K. pneumoniae. The zone of inhibition of AISNPs observed in case of E. coli was 16.08 mm, 18.65 mm against S. aureus, 10.46 mm against P. aeruginosa and 15.34 mm against K. pneumoniae. Antibiofilm activity of AISNPs demonstrated the decline of biofilm producing activity by 76% against P. aeruginosa, 74% against S. aureus, 60% against K. pneumoniae and 59% against E. coli respectively, indicating the strong antibiofilm properties of the AISNPs. It can be concluded from the present study that green synthesized AISNPs can be used as an alternate approach to combat the multiple drugresistant biofilm producing pathogens isolated from effluent.
INTRODUCTION:Environment is a multifactorial component, comprising of the natural resources like air, soil and water as essential ingredients and each of these units are directly associated with humans and all the living population found on this planet. Amongst these environmental components utilized by the humans, water is one of the most vital sources.