RETRACTED ARTICLE: Downregulation of NEAT1 reverses the radioactive iodine resistance of papillary thyroid carcinoma cell via miR-101-3p/FN1/PI3K-AKT signaling pathway

Abstract

Considering the resistance of papillary thyroid cancer (PTC) 131 I therapy, this study was designed to find a solution at molecular respect. By probing into lncRNA-NEAT1/miR-101-3p/FN1 axis and PI3K/AKT signaling pathway, this study provided a potential target for PTC therapy. 131 I-resistant cell lines were established by continuous treatment with median-lethal 131 I. Bioinformatic analysis was applied to filtrate possible lncRNA/miRNA/mRNA and related signaling pathway. Luciferase reporter assay was employed in the verification of the targeting relationship between lncRNA and miRNA as well as miRNA and mRNA. MTT assay and flow cytometry assay were performed to observe the impact of NEAT1/miR-101-3p/FN1 on cell viability and apoptosis in radioactivity iodine (RAI)-resistant PTC cell lines, respectively. Western blot and qRT-PCR were conducted to measure the expression of proteins and mRNAs in RAI-resistant PTC tissues and cells. Meanwhile, endogenous PTC mice model were constructed, in order to verify the relation between NEAT1 and RAI-resistance in vivo. NEAT1 was over-expressed in RAI-resistant PTC tissues and cell lines and could resist RAI by accelerating proliferation accompanied by suppressing apoptosis. It indicated that overexpressed NEAT1 restrained the damage of RAI to tumor in both macroscopic and microcosmic. Besides, NEAT1/miR-101-3p exhibited a negative correlation by directly targeting each other. The expression of FN1, an overexpressed downstream protein in RAI-resistance PTC tissues, could be tuned down by miR-101-3p, while the decrease could be restored by NEAT1. In conclusion, both in vitro and in vivo, NEAT1 suppression could inhibit 131 I resistance of PTC by upregulating miR-101-3p/FN1 expression and inactivated PI3K/AKT signaling pathway both in vitro and in vivo.

STAT3-induced up-regulation of lncRNA NEAT1 as a ceRNA facilitates abdominal aortic aneurysm formation by elevating TULP3

“…However, NEAT1 was also reported to interact with many other miRNAs. For example, NEAT1 was reported to sponge miR-101 in non-small cell lung cancer [34], papillary thyroid carcinoma [35], hepatocellular carcinoma [36] and breast cancer [11]. Besides, NEAT1 sponged miR-124 in neuroblastoma [37], retinoblastoma [38], Alzheimer's disease [38] and nasopharyngeal carcinoma [39].…”

MicroRNA-384 Inhibits the Progression of Papillary Thyroid Cancer by Targeting PRKACB

“…MicroRNAs are small noncoding RNAs which could negatively regulate the expression of the target genes by directly binding their 3′-UTRs [24]. It has been found that the deregulation of miRNA expression is a common feature of many types of human cancers, including thyroid cancer [25,26]. Accumulated evidence has demonstrated that the aberrant expression of miRNAs plays crucial roles in cancer initiation and progression [27][28][29][30].…”

Retracted Article: Long non-coding RNA NEAT1 accelerates cell progression in cervical cancer by regulating the miR-889-3p/E2F7 axis through the activation of the PI3K/AKT pathway

“…The suppression of NEAT1 lowers the radioactive iodine resistance of the cells by targeting miR-101-3p to regulate FN1 in papillary thyroid carcinoma through the alteration of PI3K-AKT signaling. 27 Therefore, we suggested that NEAT1 could modulate cell behavior by interacting with a specic target gene in CC. vitro and in vivo by targeting DAB2IP in esophageal squamous cell carcinomas.…”