RETRACTED ARTICLE: Enhanced suppression of tumor growth using a combination of NK4 plasmid DNA-PEG engrafted cationized dextran complex and ultrasound irradiation

Hosseinkhani, Hossein; Kubota, Toshihiro; Matsumoto, Kunio; Nakamura, Toshikazu; Tabata, Yasuhiko

doi:10.1038/sj.cgt.7700918

Cited by 41 publications

(14 citation statements)

References 53 publications

(47 reference statements)

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“…Animal study also confirmed that HGC nanoparticles could be used as a potent gene delivery vehicle in vivo [44]. The complexation with poly (ethylene glycol) (PEG) -engrafted cationized dextran in combination with ultrasound (US) irradiation is a promising way to target the NK4 plasmid DNA to the tumor for gene expression [45]. Therefore, developments of ORF nanparticles are underway in our laboratory to for achieving efficient supplementation delivery.…”

Section: Discussionmentioning

confidence: 99%

Gamma-oryzanol rich fraction regulates the expression of antioxidant and oxidative stress related genes in stressed rat's liver

et al. 2010

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BackgroundGamma-oryzanol (OR), a phytosteryl ferulate mixture extracted from rice bran oil, has a wide spectrum of biological activities in particular, it has antioxidant properties.MethodsThe regulatory effect of gamma-oryzanol rich fraction (ORF) extracted and fractionated from rice bran using supercritical fluid extraction (SFE) in comparison with commercially available OR on 14 antioxidant and oxidative stress related genes was determined in rat liver. Rats were subjected to a swimming exercise program for 10 weeks to induce stress and were further treated with either ORF at 125, 250 and 500 mg/kg or OR at 100 mg/kg in emulsion forms for the last 5 weeks of the swimming program being carried out. The GenomeLab Genetic Analysis System (GeXPS) was used to study the multiplex gene expression of the selected genes.ResultsUpon comparison of RNA expression levels between the stressed and untreated group (PC) and the unstressed and untreated group (NC), seven genes were found to be down-regulated, while seven genes were up-regulated in PC group compared to NC group. Further treatment of stressed rats with ORF at different doses and OR resulted in up-regulation of 10 genes and down regulation of four genes compared to the PC group.ConclusionsGamma-oryzanol rich fraction showed potential antioxidant activity greater than OR in the regulation of antioxidants and oxidative stress gene markers.

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Section: Discussionmentioning

confidence: 99%

Gamma-oryzanol rich fraction regulates the expression of antioxidant and oxidative stress related genes in stressed rat's liver

et al. 2010

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“…These cationic polysaccharides were prepared by reductive amination of the oligoamine and periodate-oxidized polysaccharides. Although most of these cationic conjugates formed stable complexes with pDNAs as determined by ethidium bromide quenching assay, only the dextran-spermine- (D-SPM- ) based polycations were found to be highly effective in transfecting various cell lines [25–31]. Dextran-spermine conjugates have also been modified with increasing amounts of polyethylene glycol chains (PEG).…”

Section: Introductionmentioning

confidence: 99%

Gene Transfer into the Lung by Nanoparticle Dextran-Spermine/Plasmid DNA Complexes

Abdullah

Yeo

Hosseinkhani

et al. 2010

Journal of Biomedicine and Biotechnology

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A novel cationic polymer, dextran-spermine (D-SPM), has been found to mediate gene expression in a wide variety of cell lines and in vivo through systemic delivery. Here, we extended the observations by determining the optimal conditions for gene expression of D-SPM/plasmid DNA (D-SPM/pDNA) in cell lines and in the lungs of BALB/c mice via instillation delivery. In vitro studies showed that D-SPM could partially protect pDNA from degradation by nuclease and exhibited optimal gene transfer efficiency at D-SPM to pDNA weight-mixing ratio of 12. In the lungs of mice, the levels of gene expression generated by D-SPM/pDNA are highly dependent on the weight-mixing ratio of D-SPM to pDNA, amount of pDNA in the complex, and the assay time postdelivery. Readministration of the complex at day 1 following the first dosing showed no significant effect on the retention and duration of gene expression. The study also showed that there was a clear trend of increasing size of the complexes as the amount of pDNA was increased, where the sizes of the D-SPM/pDNA complexes were within the nanometer range.

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“…We have reported that the dextran-spermine based polycations have been found to be highly effective as nonviral vectors in transfecting many different cells in vitro. [11][12][13][14][15][16][17] Our recent study also demonstrated that electrospun poly (glycolic acid) (PGA)/ collagen nanofibers significantly enhanced cell adhesion compared with PGA/collagen microfibers. 18 Based on these results, the present study aims to fabricate a tissue-like 3D transgene cell construct for use as a therapeutic cell-based gene delivery system or as an in vitro model system for testing genetic manipulations to understand the effects of gene expression on tissue development.…”

Section: Introductionmentioning

confidence: 99%

Development of 3D in vitro platform technology to engineer mesenchymal stem cells

Hosseinkhani¹,

Chen²,

Subramani³

et al. 2012

IJN

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This study aims to develop a three-dimensional in vitro culture system to genetically engineer mesenchymal stem cells (MSC) to express bone morphogenic protein-2. We employed nanofabrication technologies borrowed from the spinning industry, such as electrospinning, to mass-produce identical building blocks in a variety of shapes and sizes to fabricate electrospun nanofiber sheets comprised of composites of poly (glycolic acid) and collagen. Homogenous nanoparticles of cationic biodegradable natural polymer were formed by simple mixing of an aqueous solution of plasmid DNA encoded bone morphogenic protein-2 with the same volume of cationic polysaccharide, dextran-spermine. Rat bone marrow MSC were cultured on electrospun nanofiber sheets comprised of composites of poly (glycolic acid) and collagen prior to the incorporation of the nanoparticles into the nanofiber sheets. Bone morphogenic protein-2 was significantly detected in MSC cultured on nanofiber sheets incorporated with nanoparticles after 2 days compared with MSC cultured on nanofiber sheets incorporated with naked plasmid DNA. We conclude that the incorporation of nanoparticles into nanofiber sheets is a very promising strategy to genetically engineer MSC and can be used for further applications in regenerative medicine therapy.

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RETRACTED ARTICLE: Enhanced suppression of tumor growth using a combination of NK4 plasmid DNA-PEG engrafted cationized dextran complex and ultrasound irradiation

Cited by 41 publications

References 53 publications

Gamma-oryzanol rich fraction regulates the expression of antioxidant and oxidative stress related genes in stressed rat's liver

Gamma-oryzanol rich fraction regulates the expression of antioxidant and oxidative stress related genes in stressed rat's liver

Gene Transfer into the Lung by Nanoparticle Dextran-Spermine/Plasmid DNA Complexes

Development of 3D in vitro platform technology to engineer mesenchymal stem cells

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