RETRACTED ARTICLE: External Qi of Yan Xin Qigong induces cell death and gene expression alterations promoting apoptosis and inhibiting proliferation, migration and glucose metabolism in small-cell lung cancer cells

Abstract

Small-cell lung cancer (SCLC) is a highly malignant carcinoma with poor long-term survival. Effective treatment remains highly demanded. In the present study, we demonstrated that External Qi of Yan Xin Qigong (YXQ-EQ) exerted potent cytotoxic effect towards SCLC cell line NCI-H82 via induction of apoptosis. Global gene expression profiling identified 39 genes whose expression was altered by YXQ-EQ in NCI-82 cells. Among them, semi-quantitative RT-PCR and real-time qPCR analyses confirmed that the gene expression levels of apoptotic proteins death-associated protein kinase 2 and cell death-inducing DFFA-like effector b were upregulated, whereas that of oncoproteins DEK and MYCL1, cell migration-promoting proteins CD24 and integrin-alpha 9, and glycolytic enzyme aldolase A were downregulated. These findings suggest that YXQ-EQ may exert anticancer effect through modulating gene expression in a way that facilitates cancer cell apoptosis while represses proliferation, metastasis, and glucose metabolism.

Anti-inflammatory effect on genes expression after four days of Qigong training in peripheral mononuclear blood cells in healthy women

“…There is also data available in the subject literature which shows an induction of apoptosis caused by qigong in different cells such as colorectal cancer cells [10,38] and [39]. In the presented study, upregulation of HSF-1 mRNA and antiapoptotic HSPA1A mRNA were also observed.…”

External Qi of Yan Xin Qigong Inhibits Activation of Akt, Erk/12 and NF-ĸB and Induces Cell Cycle Arrest and Apoptosis in Colorectal Cancer Cells

“…The concept “External Qi of Qigong” refers to the technology and ability of “Qi deployment” therapy and health preservation of traditional Chinese medicine [4,5,6,7]. External Qi therapy has been used as one of the medical practices in China for thousands of years and is under management of the Chinese health authorities [8].…”

“…A great amount of YXQ-EQ experimental studies have shown significant effects of YXQ-EQ on structure and properties of DNA, RNA, protein and lipid molecules [10,14,15,16,17,18,19]. YXQ-EQ has been shown to regulate signaling pathways and gene expression in normal and malignant cells [4,5,6,7,18]. YXQ-EQ exerts potent cytotoxic effect on a variety of cancer cells and the underlying molecular mechanisms have recently begun to be addressed [4,5,6,7].…”

“…YXQ-EQ has been shown to regulate signaling pathways and gene expression in normal and malignant cells [4,5,6,7,18]. YXQ-EQ exerts potent cytotoxic effect on a variety of cancer cells and the underlying molecular mechanisms have recently begun to be addressed [4,5,6,7]. No cytotoxicity to normal cells was observed in these studies with YXQ-EQ.…”

Einfluss des therapeutischen Qi auf die Herzratenvariabilität bei Akupunktur: Eine randomisierte kontrollierte Studie

“…Der positive Einfluss von Qi Gong wurde einerseits bei den Praktizierenden selbst beobachtet [7,8], andererseits führte durch das Qi Gong hervorgebrachte externe Qi bei verschiedenen Krebszelllinien zur Apoptose [9,10,11]. Dementsprechend wird bei Anwendung von Akupunktur in der Chinesischen Medizin versucht, das Qi des Patienten positiv zu beeinflussen [12].…”