RETRACTED ARTICLE: GPX8 is transcriptionally regulated by FOXC1 and promotes the growth of gastric cancer cells through activating the Wnt signaling pathway

Chen, Hong; Xu, Lu; Shan, Zhili; Shu, Chen; Hu, Hao

doi:10.1186/s12935-020-01692-z

Cited by 28 publications

(27 citation statements)

References 24 publications

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“…PLEK2 is also involved in cancer invasion and metastasis in gallbladder cancer [26], pancreatic cancer [27], non-small cell lung cancer [28]. Consistent with our ndings, GPX8 is also identi ed as a prognostic biomarker of gastric cancer [29], and is essential for tumor aggressiveness in breast cancer [30], non-small cell lung cancer [31], and gastric cancer [32]. FSCN1 was implicated in nasopharyngeal carcinoma [33] and non-small cell lung cancer [34].…”

Section: Discussionsupporting

confidence: 83%

A cancer stemness-related eight-gene signature for predicting prognosis and malignant degree of lung adenocarcinoma

Cheng

Yao

Fang

et al. 2022

Preprint

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To identify a cancer stemness-related prognostic signature, we analyzed RNA-seq data of patients with lung adenocarcinoma from the TCGA and GEO cohort using univariate Cox and LASSO analysis. Eight genes (PLEK2, GPX8, TCN1, PERP, CD79A, PAX5, FSCN1, and CNNM1) were eventually identified to develop a cancer stemness-related signature. AUCs for 5-year overall survival were 0.681, 0.673, and 0.774 in the training, validation, and test sets, respectively. Kaplan-Meier curve analysis showed that the high-risk group has a poor survival as compared with the low-risk group in the training set, validation set, and test set, respectively (P < 0.05). Six genes (PLEK2, GPX8, TCN1, PERP, FSCN1, and CNNM1) were significantly upregulated in the cancerous tissue compared with in the normal tissue (P < 0.001), and correlated with an unwanted prognosis (P < 0.05). We constructed a prognostic nomogram combining the cancer stemness-related signature with TNM staging to precisely assess the prognosis of the patient with lung adenocarcinoma. Finally, we observed that the stemness-related gene signature also could predict the malignant levels of lung adenocarcinoma possibly via hypoxia, epithelial-mesenchymal transition and angiogenesis. Conclusively, this study developed a novel cancer stemness-related gene signature, and identified six pivotal genes which can serve as potential therapeutic targets in the treatment of lung cancer.

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Section: Discussionsupporting

confidence: 83%

A cancer stemness-related eight-gene signature for predicting prognosis and malignant degree of lung adenocarcinoma

Cheng

Yao

Fang

et al. 2022

Preprint

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“…haematobium eggs leads to urothelial hyperplasia, a potentially pre-cancerous state [ 18 ]. Proteins including metalloreductase ( STEAP4 ) [ 41 ], platelet-activating factor acetylhydrolase ( Pla2g7 ) [ 42 ], disabled homolog 2 ( Dab2 ) [ 43 ], peptidyl-prolyl cis-trans isomerase ( Fkbp14 ) [ 44 ], stabilin-1 ( Stab1 ) [ 45 , 46 ], lysyl oxidase homolog 2 ( Loxl2 ) [ 47 ], U4/U6 small nuclear ribonucleoprotein ( Prpf3 ) [ 48 ], latent-transforming growth factor beta-binding protein ( Ltbp3 ) [ 49 ], RNA-binding protein ( Nob1 ) [ 50 ], isoform 1 of RNA-binding protein ( Raly ) [ 51 ], and probable glutathione peroxidase 8 ( Gpx8 ) [ 52 ] are all expressed in many different malignant tissues, including bladder tissue, and are linked with increased cell proliferation, oncogenic properties, and poor cancer prognosis. This is in line with our observations, as all these proteins were more highly expressed in egg-injected mice bladder tissue.…”

Section: Discussionmentioning

confidence: 99%

Host tissue proteomics reveal insights into the molecular basis of Schistosoma haematobium-induced bladder pathology

et al. 2022

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Urogenital schistosomiasis remains a major public health concern worldwide. In response to egg deposition, the host bladder undergoes gross and molecular morphological changes relevant for disease manifestation. However, limited mechanistic studies to date imply that the molecular mechanisms underlying pathology are not well-defined. We leveraged a mouse model of urogenital schistosomiasis to perform for the first time, proteome profiling of the early molecular events that occur in the bladder after exposure to S. haematobium eggs, and to elucidate the protein pathways involved in urogenital schistosomiasis-induced pathology. Purified S. haematobium eggs or control vehicle were microinjected into the bladder walls of mice. Mice were sacrificed seven days post-injection and bladder proteins isolated and processed for proteome profiling using mass spectrometry. We demonstrate that biological processes including carcinogenesis, immune and inflammatory responses, increased protein translation or turnover, oxidative stress responses, reduced cell adhesion and epithelial barrier integrity, and increased glucose metabolism were significantly enriched in S. haematobium infection. S. haematobium egg deposition in the bladder results in significant changes in proteins and pathways that play a role in pathology. Our findings highlight the potential bladder protein indicators for host-parasite interplay and provide new insights into the complex dynamics of pathology and characteristic bladder tissue changes in urogenital schistosomiasis. The findings will be relevant for development of improved interventions for disease control.

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“…This is consistent with the observation that mouse bladder wall injection with S. haematobium eggs leads to urothelial hyperplasia, a potentially pre-cancerous state [18]. Proteins including metalloreductase ( STEAP4 ) [44], platelet-activating factor acetylhydrolase ( Pla2g7 ) [45], disabled homolog 2 ( Dab2 ) [46], peptidyl-prolyl cis-trans isomerase ( Fkbp14 ) [47], stabilin-1 ( Stab1 ) [48, 49], lysyl oxidase homolog 2 ( Loxl2 ) [50], U4/U6 small nuclear ribonucleoprotein ( Prpf3 ) [51], latent-transforming growth factor beta-binding protein ( Ltbp3 ) [52], RNA-binding protein ( Nob1 ) [53], isoform 1 of RNA-binding protein ( Raly ) [54], and probable glutathione peroxidase 8 ( Gpx8 ) [55] are all expressed in many different malignant tissues, including bladder tissue, and are linked with increased cell proliferation, oncogenic properties, and poor cancer prognosis. This is in line with our observations, as all these proteins were more highly expressed in egg-injected mice bladder tissue.…”

Section: Discussionmentioning

confidence: 99%

Host tissue proteomics reveal insights into the molecular basis of Schistosoma haematobium-induced bladder pathology

Osakunor

Ishida

Lamanna

et al. 2021

Preprint

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BackgroundUrogenital schistosomiasis remains a major public health concern worldwide. In response to egg deposition, the host bladder undergoes gross and molecular morphological changes relevant for disease manifestation. However, limited mechanistic studies to date imply that the molecular mechanisms underlying pathology have not been well-defined. We leveraged a mouse model of urogenital schistosomiasis to perform for the first time, proteome profiling of the early molecular events that occur in the bladder after exposure to S. haematobium eggs, and to elucidate the protein pathways involved in urogenital schistosomiasis-induced pathology.MethodsPurified S. haematobium eggs or control vehicle were microinjected into the bladder walls of mice. Mice were sacrificed seven days post-injection and bladder proteins isolated and processed for proteome profiling using mass spectrometry.ResultsWe demonstrate that biological processes including carcinogenesis, immune and inflammatory responses, increased protein translation or turnover, oxidative stress responses, reduced cell adhesion and epithelial barrier integrity, and increased glucose metabolism were significantly enriched in S. haematobium infection.ConclusionS. haematobium egg deposition in the bladder results in significant changes in proteins and pathways that play a role in pathology. Our findings highlight the potential bladder protein indicators for host-parasite interplay and provide new insights into the complex dynamics of pathology and characteristic bladder tissue changes in urogenital schistosomiasis. The findings will be relevant for development of improved interventions for disease control.Author summaryThe molecular mechanisms underlying the urinary and genital pathology from urogenital schistosomiasis have not been well-defined. This has mainly been due to limited mechanistic studies and the lack of a suitable animal model for S. haematobium infection. We leveraged a mouse model of urogenital schistosomiasis, along with proteomics analysis, to determine the early molecular events that occur in the bladder after exposure to S. haematobium eggs, and to define protein pathways that may be involved in morbidity from urogenital schistosomiasis infection. Our results show that S. haematobium egg deposition in the bladder results in significant changes in proteins and pathways that play a role in pathology. Biological processes including carcinogenesis, immune and inflammatory responses, increased protein translation or turnover, oxidative stress responses, reduced cell adhesion and epithelial barrier integrity, and increased glucose metabolism are significantly enriched in S. haematobium infection. Our findings highlight important protein indicators of host-parasite interactions in the bladder and their association with tissue changes in urogenital schistosomiasis. The findings presented here will be relevant for development of improved interventions for disease control, and contribute to realizing the 2021-2030 NTD goals by demonstrating evidence on the early events that occur during schistosome infection..

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RETRACTED ARTICLE: GPX8 is transcriptionally regulated by FOXC1 and promotes the growth of gastric cancer cells through activating the Wnt signaling pathway

Cited by 28 publications

References 24 publications

A cancer stemness-related eight-gene signature for predicting prognosis and malignant degree of lung adenocarcinoma

A cancer stemness-related eight-gene signature for predicting prognosis and malignant degree of lung adenocarcinoma

Host tissue proteomics reveal insights into the molecular basis of Schistosoma haematobium-induced bladder pathology

Host tissue proteomics reveal insights into the molecular basis of Schistosoma haematobium-induced bladder pathology

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