RETRACTED ARTICLE: Guangxi cobra venom-derived NGF promotes the osteogenic and therapeutic effects of porous BCP ceramic

Abstract

Neuro-osteological interactions have an important role in the regulation of bone metabolism and regeneration. Neuropeptides combined with porous biphasic calcium phosphates (BCP) using protein adsorption may contribute to the acceleration of bone formation. In the present study, we investigated the effect of BCP combined with nerve growth factor (NGF) on the growth of osteoblasts in vitro and the combinational therapeutic effect on the repair of calvarial defects in vivo. NGF was separated and purified from Chinese cobra venom using a simplified three-step chromatography method. BCP combined with NGF exerted a potent effect on osteoblast differentiation, as evidenced by enhanced cell proliferation, increased ALP activity and the up-regulated expression of osteogenesis-related genes and proteins. Further, combinational therapy with BCP and NGF improved calvarial regeneration, which was superior to treatment with therapy alone, as observed using imageological and morphological examination and histological and immunohistochemical staining. The results confirmed the effect of neuro-osteological interactions through combinatorial treatment with NGF and BCP to promote osteogenesis and bone formation, which may provide an effective and economical strategy for clinical application.

Effects of exogenous nerve growth factor on the expression of BMP-9 and VEGF in the healing of rabbit mandible fracture with local nerve injury

“…The result concluded that the serum levels of NGF content were higher in the fracture combined with the brain injury group than in the control group [ 36 , 37 ]. This result was also verified by the report that NGF stimulated osteoblasts to promote bone cell growth by phosphorylation of NGF receptors [ 38 ]. Therefore, NGF, as an important bridge transmitter, could affect the metabolism of bone tissues in the nervous system and then play an important role in the healing of mandible fractures.…”

Effects of Exogenous Nerve Growth Factor on The Expression of BMP-9 and VEGF in The Healing of Rabbit Mandible Fracture With Local Nerve Injury

“…The result concluded that the serum levels of NGF content were higher in the fracture combined with the brain injury group than in the control group [35,36]. This result was also veri ed by the report that NGF stimulated osteoblasts to promote bone cell growth by phosphorylation of NGF receptors [37]. Therefore, NGF, as important bridge transmitters, could affect the metabolism of bone tissues in the nervous system and then play an important role in the healing of mandible fractures.…”

Effects of exogenous nerve growth factor on the expression of BMP-9 and VEGF in the healing of rabbit mandible fracture with local nerve injury

“…Fracture healing especially to mandible fracture due to special physiology and facial parts refers to the process of repair, which is jointly affected by nerve regulation and humoral regulation after the physiological results and functions of the bone are damaged [34]. NGF is widely distributed in central and peripheral nervous systems that not only regulate growth, development, differentiation, regeneration, and functional protein expression of neurons [35] but also stimulate osteoblasts to promote bone cell growth by NGF receptors' phosphorylation [36]. In this study, rabbit models of incomplete mandible fracture with mental neurovascular bundle were created in the NGF group, GS group, and blank group.…”