RETRACTED ARTICLE: Has_circ_0008583 modulates hepatocellular carcinoma progression through the miR-1301-3p/METTL3 pathway

Abstract

Has_circ_0008583 is reported to be involved in the progression of hepatocellular carcinoma (HCC), while its biological role in HCC remains unclear. Here, the qRT-PCR was used to detect the expression of has_circ_0008583. The CCK-8 kit was performed to measure cell proliferation. The cell migration and invasion were evaluated by Transwell. A dual-luciferase reporter assay was performed to confirm the target combination between the genes in has_circ_0008583/miR-1301-3p/METTL3 axis. The in vivo role of has_circ_0008583 was verified by murine xenograft assay. Our data showed that hsa_circ_0008583 was upregulated in HCC tissues and cells. Hsa_circ_0008583 overexpression promoted Hep3B cell proliferation, migration and invasion, but hsa_circ_0008583 silencing had an opposing influence. MiR-1301-3p is directly bound to hsa_circ_0008583 and METTL3. MiR-1301-3p overexpression or METTL3 knockdown could partially counteract hsa_circ_0008583 overexpression-mediated influence on HCC cell behaviors. In addition, hsa_circ_0008583 depletion inhibits HCC tumor growth in vivo . In conclusion, hsa_circ_0008583 promotes HCC progression through the miR-1301-3p/METTL3 axis.

RNA modifications in the progression of liver diseases: from fatty liver to cancer

“…Other circRNAs regulate m 6 Arelated proteins as well. Has_circ_0008583 sponges miR-1301-3p to promote METTL3 expression (Wang et al, 2022a), and circMAP2K4 sponges miR-139-5p to promote YTHDF1 expression (Chi et al, 2021), both of which further HCC progression.…”

Non-coding RNA methylation modifications in hepatocellular carcinoma: interactions and potential implications

“…Functioning as a miR-1305 sponge, circ-ARL3 further accentuates HBV-associated HCC tumor formation. Moreover, METTL3 mediates the effects of hsa_circ_0008583 on the behavior of HCC cells [ 97 ]. Furthermore, hsa_circ_0008583 accelerates the development of HCC via the miR-1301-3p/METTL3 axis.…”

The bidirectional interplay between ncRNAs and methylation modifications in gastrointestinal tumors

“…Knockdown of circ_0000467 upregulated DNMT3B expression and suppressed the malignant phenotype of colorectal cancer cells [98]. In HCC, circ_0008583 also promoted HCC cancer progression by inhibiting miR-1301-3p and up-regulating METTL3 [99]. In addition to acting as miRNA sponges, circRNAs can also regulate digestive tumor progression in other ways.…”