RETRACTED ARTICLE: Overexpression of microRNA-221 promotes the differentiation of stem cells from human exfoliated deciduous teeth to neurons through activation of Wnt/β-catenin pathway via inhibition of CHD8

Wen, Bing; He, Chenjiang; Zhang, Qin; Zhang, Fanglin; Li, Na; Pan, Yan; Deng, Mengting; Wang, Yue; Li, Jianping; Qiu, Jiaxuan

doi:10.1080/15384101.2020.1816308

Cited by 6 publications

(3 citation statements)

References 37 publications

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“…Differentiation to odontogenic/osteogenic lineages was the most frequently investigated [34 studies], three studies [ 43 – 45 ] investigated neuronal differentiation, two [ 42 , 46 ] investigated angiogenic (endothelial) differentiation, and one [ 47 ] investigated differentiation to myogenic lineage. Regarding the stem cell type, 35 studies had recruited hDPSCs and five studies [ 42 – 44 , 48 , 49 ] had used SHEDs (Table 1 ). In all studies of this group, hDPSCs were isolated from human third molar or premolar teeth, and SHEDs were harvested from human deciduous teeth of healthy donors.…”

Section: Resultsmentioning

confidence: 99%

“…Among them, hsa-miR-140-5p, hsa-miR-218 and hsa-miR-143 family were more frequently reported suppressing the odontogenic/osteogenic differentiation of hDP-MSCs [ 36 , 50 – 58 ]. Two miRNAs (hsa-miR-221 and hsa-miR-124) promoted [ 44 , 45 ], and one (hsa-miR-140-5p) inhibited neuronal differentiation [ 43 ], one (hsa-miR-26a-5p) promoted [ 42 ] and another one (hsa-miR-424) suppressed angiogenic differentiation [ 46 ], and two (hsa-miR-135 and hsa-miR-143) inhibited differentiation to myogenic lineage [ 47 ].…”

Section: Resultsmentioning

confidence: 99%

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MicroRNAs-mediated regulation of the differentiation of dental pulp-derived mesenchymal stem cells: a systematic review and bioinformatic analysis

et al. 2023

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Background Human dental pulp-derived mesenchymal stem cells (hDP-MSCs), which include human dental pulp stem cells (hDPSCs) and stem cells from human exfoliated deciduous teeth (SHEDs), are promising cell sources for regenerative therapies. Nevertheless, a lack of knowledge relating to the mechanisms regulating their differentiation has limited their clinical application. microRNAs (miRNAs) are important regulatory molecules in cellular processes including cell differentiation. This systematic review aims to provide a panel of miRNAs that regulate the differentiation of hDP-MSCs including hDPSCs and SHEDs. Additionally, bioinformatic analyses were conducted to discover target genes, signaling pathways and gene ontologies associated with the identified miRNAs. Methods A literature search was performed in MEDLINE (via PubMed), Web of Science, Scopus, Embase and Cochrane Library. Experimental studies assessing the promotive/suppressive effect of miRNAs on the differentiation of hDP-MSCs and studies evaluating changes to the expression of miRNAs during the differentiation of hDP-MSCs were included. miRNAs involved in odontogenic/osteogenic differentiation were then included in a bioinformatic analysis. A miRNA-mRNA network was constructed, and Gene Ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed. A protein–protein interaction (PPI) network was also constructed. Results Of 766 initially identified records through database searching, 42 and 36 studies were included in qualitative synthesis and bioinformatic analyses, respectively. Thirteen miRNAs promoted and 17 suppressed odontogenic/osteogenic differentiation of hDP-MSCs. hsa-miR-140-5p, hsa-miR-218 and hsa-miR-143 were more frequently reported suppressing the odontogenic/osteogenic differentiation of hDP-MSCs. hsa-miR-221 and hsa-miR-124 promoted and hsa-miR-140-5p inhibited neuronal differentiation, hsa-miR-26a-5p promoted and hsa-miR-424 suppressed angiogenic differentiation, and hsa-miR-135 and hsa-miR-143 inhibited differentiation within myogenic lineages. A miRNA-mRNA network including 1890 nodes and 2171 edges was constructed. KEGG pathway analysis revealed MAPK, PI3K-Akt and FoxO as key signaling pathways involved in the odontogenic/osteogenic differentiation of hDP-MSCs. Conclusions The findings of this systematic review support the potential application of the specific miRNAs to regulate the directed differentiation of hDP-MSCs in the field of regenerative therapies.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

MicroRNAs-mediated regulation of the differentiation of dental pulp-derived mesenchymal stem cells: a systematic review and bioinformatic analysis

et al. 2023

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show abstract

“…Some scholars transfected hDPSCs with miR-124 to observe the changes of neural progenitor cells and protein levels, and found that the expression of neural marker Nestin could be changed, which opened up broad prospects for the treatment and replacement of SGN ( Mehri-Ghahfarrokhi et al, 2019 ). Chromatin unwinding DNA-binding protein 8(CHD8) inhibited the onset of autism spectrum disorder (ASD), and the upregulation of miR-221 activated the Wnt/β-catenin pathway by binding to CHD8, which facilitating the differentiation from SHEDs to neurons ( Wen et al, 2020 ). Autism is a childhood neurodevelopmental disorder with complex genetic origin.…”

Section: Ncrnas and The Differentiation Of Dmscsmentioning

confidence: 99%

Progress in the Study of Non-Coding RNAs in Multidifferentiation Potential of Dental-Derived Mesenchymal Stem Cells

Zeng

2022

Front. Genet.

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For decades, the desire for tissue regeneration has never been quenched. Dental-derived mesenchymal stem cells (DMSCs), with the potential of self-renewal and multi-directional differentiation, have attracted much attention in this topic. Growing evidence suggests that non-coding RNAs (ncRNAs) can activate various regulatory processes. Even with a slight decrease or increase in expression, ncRNAs can weaken or even subvert cellular fate. Therefore, a systematic interpretation of ncRNAs that guide the differentiation of DMSCs into cells of other tissue types is urgently needed. In this review, we introduce the roles of ncRNAs in the differentiation of DMSCs, such as osteogenic differentiation, odontogenic differentiation, neurogenic differentiation, angiogenic differentiation and myogenic differentiation. Additionally, we illustrate the regulatory mechanisms of ncRNAs in the differentiation of DMSCs, such as epigenetic regulation, transcriptional regulation, mRNA modulation, miRNA sponges and signalling. Finally, we summarize the types and mechanisms of ncRNAs in the differentiation of DMSCs, such as let-7 family, miR-17∼92 family, miR-21, lncRNA H19, lncRNA ANCR, lncRNA MEG3, circRNA CDR1as and CircRNA SIPA1L1. If revealing the intricate relationship between ncRNAs and pluripotency of DMSCs 1 day, the application of DMSCs in regenerative medicine and tissue engineering will be improved. Our work could be an important stepping stone towards this future.

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Autophagy and apoptosis cascade: which is more prominent in neuronal death?

Gupta

Ambasta

Kumar

2021

Cell. Mol. Life Sci.

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RETRACTED ARTICLE: Overexpression of microRNA-221 promotes the differentiation of stem cells from human exfoliated deciduous teeth to neurons through activation of Wnt/β-catenin pathway via inhibition of CHD8

Cited by 6 publications

References 37 publications

MicroRNAs-mediated regulation of the differentiation of dental pulp-derived mesenchymal stem cells: a systematic review and bioinformatic analysis

MicroRNAs-mediated regulation of the differentiation of dental pulp-derived mesenchymal stem cells: a systematic review and bioinformatic analysis

Progress in the Study of Non-Coding RNAs in Multidifferentiation Potential of Dental-Derived Mesenchymal Stem Cells

Autophagy and apoptosis cascade: which is more prominent in neuronal death?

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