RETRACTED ARTICLE: Silencing of PTEN inhibits the oxidative stress damage and hippocampal cell apoptosis induced by Sevoflurane through activating MEK1/ERK signaling pathway in infant rats

Abstract

Phosphatase and tensin homolog (PTEN) is a suppressive player in tumor but its concrete role in oxidative stress (OS) damage and cell apoptosis remains much exploration. Thus, this study is conducted to explore the participation of PTEN and its mechanisms in OS damage and cell apoptosis in hippocampal cells. Infant rats were grouped into normal, Sevo, Sevo + si-negative control (NC), Sevo + si-PTEN and Sevo + si-PTEN + PD (MEK1/ERK signaling pathway inhibitor) groups. Infant hippocampal cells were grouped into blank, Sevo, Sevo + si-NC, Sevo + si-PTEN and Sevo + si-PTEN + PD groups. The expressions of PTEN and MEK1/ERK signaling pathway-related proteins were determined. OSrelated indices in hippocampal tissues and cells were detected. Cell apoptosis was detected by flow cytometry. Sevoflurane up-regulated PTEN expression and silencing of PTEN activates MEK1/ERK signaling pathway in hippocampal tissues and cells of infant rats. Silencing of PTEN alleviated hippocampal tissue pathological status and inhibited sevoflurane-induced cell apoptosis in hippocampal tissues of infant rats. Silencing of PTEN alleviated OS damage in hippocampal tissues of infant rats. Silencing of PTEN inhibited sevoflurane-induced apoptosis after OS damage in hippocampal cells of infant rats. Silencing of PTEN reduced sevoflurane-induced OS damage in hippocampal cells of infant rats. Our study demonstrates that PTEN silencing inhibits the OS damage and cell apoptosis in hippocampal cells induced by Sevoflurane through activating MEK1/ERK signaling pathway in infant rats.

Tandem mass tag-based quantitative proteomic analysis of effects of multiple sevoflurane exposures on the cerebral cortex of neonatal and adult mice

“…In neonatal mice exposed to sevoflurane anesthesia, elevated PTEN expression may indicate neuronal loss in key brain regions that mediate cognitive function during neurodevelopment, and excessive activation of autophagy leads to autophagic programmed cell death ( 23 , 24 ). The observed alterations in PTEN expression are consistent with several previous studies ( 25 , 26 ). MAP2 exhibits microtubule stabilization activities that involve neurogenesis, morphogenesis, and migration for the development of axons and dendrites.…”

“…Combined with our findings exhibited in the BP of enriched GO terms (Figure 4A), multiple exposures to sevoflurane anesthesia could induce dysfunctional synaptic transmission in neonatal mice by increasing CHGB expression. PTEN protein is known to play a potential role in regulating the structure and plasticity of neurons, which hinders the Akt/mTOR signaling pathway to decrease the growth and proliferation of neurons and consistent with several previous studies (25,26). MAP2 exhibits microtubule stabilization activities that involve neurogenesis, morphogenesis, and migration for the development of axons and dendrites.…”

Activation of PTEN/P13K/AKT Signaling Pathway by miRNA-124-3p-Loaded Nanoparticles to Regulate Oxidative Stress Attenuates Cardiomyocyte Regulation and Myocardial Injury

“…Multiple researches have indicated the great importance of miR-124-3p in cardiovascular diseases [ 25 ]. The PTEN gene is the first novel tumor suppressor gene with specific phosphatase activity that was cloned in 1997 and can effectively inhibit the growth of tumor cells [ 26 ]. The current research elucidated that the target gene of miR-124-3p PTEN mediated the progression of myocardial infarction.…”

Exosomal microRNA‐139‐5p from mesenchymal stem cells accelerates trophoblast cell invasion and migration by motivation of the ERK/MMP‐2 pathway via downregulation of protein tyrosine phosphatase

“…In the current study, microarray analysis and dual‐luciferase reporter gene assay displayed that miR‐139‐5p was able to mediate PTEN negatively. Through triggering the MEK1/ERK pathway, PTEN silencing could hinder the oxidative stress injury and apoptosis in hippocampal cells in infant rats caused by Sevoflurane 37 . Avermectin regulated the PI3K‐ERK pathway negatively to reduce respiratory burst in carp by activating PTEN demethylation 38 .…”

“…Through triggering the MEK1/ERK pathway, PTEN silencing could hinder the oxidative stress injury and apoptosis in hippocampal cells in infant rats caused by Sevoflurane. 37 Avermectin regulated the PI3K-ERK pathway negatively to reduce respiratory burst in carp by activating PTEN demethylation. 38 Down-regulation of a trophoblast-derived MMP-9, and the main protein hydrolase ERK/MMP-2 pathways could lead to invasion disorders of trophoblasts in the PE placenta.…”