RETRACTED ARTICLE: The circular RNA hsa-circ-0072309 plays anti-tumour roles by sponging miR-100 through the deactivation of PI3K/AKT and mTOR pathways in the renal carcinoma cell lines

Chen, Tao; Shao, Shixiu; Li, Wenxian; Liu, Yong; Cao, Yimei

doi:10.1080/21691401.2019.1657873

Cited by 45 publications

(46 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In human intracranial aneurysms, circ 0072309 acts as a ceRNA to affect discoid domain receptor 2 and systemic amino acid transporter 2 through miR-519e-5p and miR-516b-5p [10]. In renal carcinoma, circ 0072309 exerts anti-tumor roles by sponging miR-100 [13]. In our present study, we predicted that miR-580-3p is a target of circ 0072309 by bioinformatics analysis and employed luciferase report assay and RNA pull down methods to confirm that circ 0072309 directly binds to miR-580-3p.…”

Section: Discussionmentioning

confidence: 99%

“…CircRNA has circ 0072309 was identified to be located on chromosome 5 between the base sites 38523520 and 38530768 [10]. Some reports showed that has circ 0072309 was lowly expressed in breast cancer [11,12], human intracranial aneurysms [10] and renal carcinoma [13]. However, the function of has circ 0072309 in the progression of NSCLC remains elusive.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Circular RNA hsa_circ_0072309 inhibits non-small cell lung cancer progression by sponging miR-580-3p

et al. 2020

View full text Add to dashboard Cite

Objective: Non-small cell lung cancer (NSCLC) continues to top the list of cancer mortalities worldwide. Early diagnosis and therapeutic interventions targeting NSCLC is becoming the world's significant challenge. Circular RNAs (circRNAs) are emerging as a group of potential cancer biomarkers. Materials and methods: Quantitative real-time PCR (qRT-PCR) was employed to examine the expression of circ 0072309 in NSCLC tissues and cell lines. Cell counting kit 8 (CCK-8), wound healing and Transwell assays were used to analyze cell proliferation, migration and invasion in A549 and H1299 cells. The relationship between circ 0072309 and miR-580-3 was analyzed by Luciferase reporter and RNA pull down assays. Results: We screened circ 0072309 from Gene Expression Omnibus and found that circ 0072309 was lowly expressed in NSCLC tissues and cell lines. The transfection of circ 0072309-overexpressing vector significantly suppressed the cell proliferation, migration and invasion in A549 and H1299 cells. We predicted that miR-580-3p is a target of circ 0072309 by using publicly available bioinformatic algorithms Circinteractome tool and confirmed that circ 0072309 directly bound to miR-580-3p. Furthermore, the addition of miR-580-3p mitigated the blockage of cell proliferation, migration and invasion induced by circ 0072309. Conclusions: These data showed that circ 0072309 inhibits the progression of NSCLC progression via blocking the expression of miR-580-3p. These findings revealed the anti-tumor role of circ 0072309 during the development of NSCLC and provided a novel diagnostic biomarker and potential therapy for NSCLC.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Circular RNA hsa_circ_0072309 inhibits non-small cell lung cancer progression by sponging miR-580-3p

et al. 2020

View full text Add to dashboard Cite

show abstract

“…16 Several circRNAs abnormally expressed in RCC have been discovered. 11,12 For instance, circ-ZNF609 plays a ceRNA to upregulate FOXP4 level via targeting miR-138-5p in RCC. 17 Androgen receptor could promote RCC cell metastasis by targeting the circHIAT1/miR-195-5p/29a-3p/29c-3p/CDC42 pathway.…”

Section: Discussionmentioning

confidence: 99%

“…8,9 Currently, several reports also described the functions of a few circRNAs in RCC. 10,11 For example, Chen et al 12 unveiled that circ_0072309 acts as an antitumor role via targeting miR-100 through the deactivation of PI3K/AKT and mTOR signaling in RCC. In recent years, the competing endogenous RNA (ceRNA) concept proposes that circRNAs regulate gene expression through functioning as a molecular sponge of microRNAs (miRNAs), illuminating the importance of such interactions during the tumorigenic process.…”

Section: Introductionmentioning

confidence: 99%

Circular RNA circ‐EGLN3 promotes renal cell carcinoma proliferation and aggressiveness via miR‐1299‐mediated IRF7 activation

Lin

Cai

2020

J of Cellular Biochemistry

View full text Add to dashboard Cite

Renal cell carcinoma (RCC) is a highly lethal cancer with increasing incidence worldwide. The purpose of the present study was to investigate the functions and molecular mechanisms of circular RNA (circRNA), circ‐EGLN3, in RCC progression. The expression levels of circ‐EGLN3 were assessed by a quantitative real‐time polymerase chain reaction. Kaplan‐Meier analysis was applied to uncover the prognostic role of circ‐EGLN3 in patients with RCC. Cell viability was analyzed using cell counting kit‐8 and cell apoptosis was assessed using flow cytometric experiment. Cell migratory and invasive abilities were determined by wound scratch and transwell experiments. Subcellular distribution detection was utilized to investigate the location of circ‐EGLN3. Dual‐luciferase reporter test was utilized for identifying the molecular mechanism of circ‐EGLN3. The results indicated that circ‐EGLN3 was elevated in RCC tissues and cell lines and predicted unfavorable prognosis for the patients with RCC. Silenced circ‐EGLN3 hindered cell proliferation, migration, and invasion but facilitated apoptosis of RCC cells. Ectopic expressed circ‐EGLN3 induced the opposite effects mentioned above. Mechanistically, circ‐EGLN3 was mainly located at the cytoplasm. Circ‐EGLN3 acted as a competing endogenous RNA (ceRNA) to enhance the IRF7 level via sponging miR‐1299. Moreover, circ‐EGLN3 mediated elevation of IRF7 is responsible for RCC cell proliferation and aggressiveness. Collectively, our study suggested that circ‐EGLN3 knockdown suppressed RCC progression through acting as a ceRNA to regulate the IRF7 expression by targeting miR‐1299. Circ‐EGLN3 might be a potential therapeutic target for RCC management.

show abstract

“…Differentially, circRNA threonine-speci c protein kinase 3 (circ-AKT3) overexpression was demonstrated to decrease migrated and invaded CCRCC cells [28]. CircRNA Rap guanine nucleotide exchange factor 5 (cRAPGEF5) [29] and has_circ_0072309 [30] also impeded tumor growth and metastasis in CCRCC. In conformity with these anti-cancer circRNAs, circ_RPL23A overexpression evoked cell cycle arrest, proliferation and metastasis inhibition, and apoptosis elevation.…”

Section: Discussionmentioning

confidence: 99%

Circ_RPL23A acts as a miR-1233 sponge to suppress the progression of clear cell renal cell carcinoma by promoting ACAT2

Cheng

Cao

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Clear cell renal cell carcinoma (CCRCC) is a prevalent urological carcinoma with high metastatic risk. Circular RNAs (circRNAs) have been identified as effective diagnostic and therapeutic biomarkers for CCRCC. This research aims to disclose the involvement of circRNA ribosomal protein L23a (circ_RPL23A) in CCRCC, and how it regulates carcinogenesis. Methods We performed quantitative real-time polymerase chain reaction (qRT-PCR) to examine circ_RPL23A, microRNA-1233 (miR-1233) and acetyl-coenzyme A acetyltransferase 2 (ACAT2). Cellular behavior detection included cell cycle, proliferation, apoptosis and metastasis, which were severally analyzed using propidium iodide (PI)-flow cytometry, 3-(4, 5-dimethylthiazol-2-y1)-2, 5-diphenyl tetrazolium bromide (MTT), Annexin V/PI-flow cytometry and transwell migration/invasion assays. ACAT2 and related proteins of cell cycle, epithelial-mesenchymal transition (EMT) were measured via western blot. Target relationship was analyzed via dual-luciferase reporter assay. A xenograft model was used to study circ_RPL23A action in mice. Results Both in CCRCC tissues and cells, circ_RPL23A had a down-regulated tendency. Explicitly, circ_RPL23A overexpression inhibited cell cycle, proliferation and metastasis but enhanced apoptosis of CCRCC cells, whereas these effects of circ_RPL23A were dependent on the suppression of its target miR-1233. Besides, miR-1233 could target ACAT2 and circ_RPL23A regulated ACAT2 by sponging miR-1233. Also importantly, miR-1233 was a pro-cancer gene in CCRCC via targeting ACAT2. CCRCC tumor growth was also decreased in vivo by circ_RPL23A through miR-1233/ACAT2 axis. Conclusion Altogether, the circ_RPL23A/miR-1233/ACAT2 axis in this report provides an in-depth cognition for CCRCC pathogenesis and circ_RPL23A may has pivotal value in diagnosing and treating CCRCC.

show abstract

RETRACTED ARTICLE: The circular RNA hsa-circ-0072309 plays anti-tumour roles by sponging miR-100 through the deactivation of PI3K/AKT and mTOR pathways in the renal carcinoma cell lines

Abstract: Cao (2019) The circular RNA hsa-circ-0072309 plays anti-tumour roles by sponging miR-100 through the deactivation of PI3K/AKT and mTOR pathways in the renal carcinoma cell lines, Artificial Cells,

Cited by 45 publications

References 38 publications

Circular RNA hsa_circ_0072309 inhibits non-small cell lung cancer progression by sponging miR-580-3p

Circular RNA hsa_circ_0072309 inhibits non-small cell lung cancer progression by sponging miR-580-3p

Circular RNA circ‐EGLN3 promotes renal cell carcinoma proliferation and aggressiveness via miR‐1299‐mediated IRF7 activation

Circ_RPL23A acts as a miR-1233 sponge to suppress the progression of clear cell renal cell carcinoma by promoting ACAT2

Contact Info

Product

Resources

About