RETRACTED: Biocatalytic strategies for the production of high fructose syrup from inulin

Singh, Ram Sarup; Chauhan, Kanika; Pandey, Ashok; Larroche, Christian

doi:10.1016/j.biortech.2018.03.127

Cited by 65 publications

(37 citation statements)

References 81 publications

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“…High fructose syrup (HFS) is a major nutritive sweetener presented as a mixture of glucose and fructose (Singh et al, 2018a). In modern food and beverage industries, HFS has replaced sucrose as an alternative sweetener because of its many advantages (Rippe, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…In modern food and beverage industries, HFS has replaced sucrose as an alternative sweetener because of its many advantages (Rippe, 2014). For example, HFS is easier to store than sucrose because of its high osmotic pressure and anti-drying properties (Singh et al, 2018a). Furthermore, HFS is crystallization-controlled and easy to dissolve, making it flexible for many applications, such as seasoning and baking (Zhang et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

“…Commercially, HFS is obtained from cornstarch through multi-enzymatic hydrolysis and transformation in four steps, including (a) enzymatic liquefaction or partial hydrolysis of starch with α-amylase, (b) conversion of liquefied starch into dextrose hydrolyzate by employing amylo-glucosidase, (c) isomerization of dextrose to fructose using isomerase, and (d) refinement of the final fructose product (Singh et al, 2018a). Ultra-filtration and crystallization steps, such as activated carbon decolorization, ion exchange, chromatographic separation, and evaporation, are necessary to obtain highpurity fructose (Guthrie and Morton, 2000;Chen et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Identification and Immobilization of an Invertase With High Specific Activity and Sucrose Tolerance Ability of Gongronella sp. w5 for High Fructose Syrup Preparation

et al. 2020

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Invertases catalyze the hydrolysis of sucrose into fructose and glucose and can be employed as an alternative in producing high fructose syrup. In this study, we reported the heterologous expression of an invertase gene (GspInv) of Gongronella sp. w5 in Komagataella pastoris. GspInv activity reached 147.6 ± 0.4 U/mL after 5 days of methanol induction. GspInv is invertase with a high specific activity of 2,776.1 ± 124.2 U/mg toward sucrose. GspInv showed high tolerance to sucrose (IC 50 = 1.2 M), glucose (IC 50 > 2 M), fructose (IC 50 = 1.5 M), and a variety of metal ions that make it an ideal candidate for high fructose syrup production. A carbohydratebinding module was sequence-optimized and fused to the N-terminus of GspInv. The fusion protein had the highest immobilization efficiency at room temperature within 1 h adsorption, with 1 g of cellulose absorption up to 8,000 U protein. The celluloseimmobilized fusion protein retained the unique properties of GspInv. When applied in high fructose syrup preparation by using 1 M sucrose as the substrate, the sucrose conversion efficiency of the fused protein remained at approximately 95% after 50 h of continuous hydrolysis on a packed bed reactor. The fused protein can also hydrolyze completely the sucrose in sugarcane molasses. Our results suggest that GspInv is an unusual invertase and a promising candidate for high fructose syrup preparation.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification and Immobilization of an Invertase With High Specific Activity and Sucrose Tolerance Ability of Gongronella sp. w5 for High Fructose Syrup Preparation

et al. 2020

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“…It is an important agricultural by‐product and commonly used in the food sector, drug transport systems, tissue engineering, pharmaceutical and cosmetic products 1‐5 . In the food sector, it is used as dietary fiber to increase the nutritional value of products and is one of the most beneficial natural probiotics 6‐11 . Additionally, inulin can be considered a renewable material in bioprocesses for biofuel production.…”

Section: Introductionmentioning

confidence: 99%

Biodegradable super porous inulin cryogels as potential drug carrier

Ari

Şahiner

2020

Polymers for Advanced Techs

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Here, chemically crosslinked super porous inulin cryogels were synthesized by the cryogelation method using divinyl sulfone (DVS) at various mol ratios of 75% to 150% inulin repeating units. Inulin cryogel prepared at 75 mol% DVS crosslinking showed a maximum swelling capacity of 1842 ± 159% in phosphate buffer saline (PBS, pH 7.4). The hydrolytic degradation of inulin cryogels prepared at different ratios were investigated at 37.5 Cat pH 1, 7.4, and 9. Depending on the degree of crosslinking, it was found that inulin cryogels were completely degraded in 1 to 6 days at pH 1, and the degradation continued at pH 7.4 and 9 for up to 21 days. Inulin cryogel with 75 mol% crosslinker had 61% ± 7% and 45% ± 2% weight losses at pH 7.4 and pH 9, respectively, at end of 21 days. Also, 75% crosslinked inulin cryogel was conjugated with amoxicillin trihydrate (AMX) drug and released 65.15 ± 5.35 mg/g of AMX at pH 1 at end of 24 hours. At pH 7.4 and 9, the AMX released amount were determined as 64.83 ± 6.19 and 55.57 ± 4.00 mg/g, respectively, in 120 hours. Furthermore, inulin cryogels were determined to be blood compatible materials via hemolytic tests with hemolysis ratio of 1.08% ± 0.14% and a blood clotting index of 80.67% ± 1.86%. K E Y W O R D S biodegradable polymer network, blood compatible drug carrier, inulin cryogel 1 | INTRODUCTION Inulin is a plant-based linear biopolymer naturally occurring in many plants such as chicory, asparagus, wheat, onion, garlic, banana, etc. It is an important agricultural by-product and commonly used in the food sector, drug transport systems, tissue engineering, pharmaceutical and cosmetic products. 1-5 In the food sector, it is used as dietary fiber to increase the nutritional value of products and is one of the most beneficial natural probiotics. 6-11 Additionally, inulin can be considered a renewable material in bioprocesses for biofuel production. The biocompatible, biodegradable, renewable, and hydrophilic structure, along with natural and plant-based origin, make inulin a very attractive material for diverse applications. 12-14 Cryogels are distinctive types of hydrogels with faster response time to external stimuli due to their highly macro porous interconnected matrices that can be obtained by crosslinking monomeric or polymeric precursors in the partially frozen phase under cryogenic conditions. 15,16 The size and the extent of pores in cryogels can be controlled by various parameters, for example, the amount of water, cooling rate, the reaction temperature, monomer/polymer concentration, the amount of crosslinker, the amount of catalyst, etc. 17-21 Ascryogels exhibit faster response time and mechanical strength compared to normal hydrogels, they offer many advantages in common applications including environmentally related, that is, separation, actuation, and sensors, tissue engineering and as cell carrier in biotechnology due to their super-porous structures. 22-27 Moreover, because it is possible to prepare cryogels from natural sources and they are biodegradable, they can...

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“…The technical and functional characteristics of fructose over other sweetener are as follows: low molecular weight, flavour enhancer, smooth consistency, good humectant, high osmotic pressure, high solubility, no crystal formation, stable in acidic foods, etc. (Singh et al ). Also, fructose plays beneficial physiological roles in the human body such as (i) bypassing metabolic pathway of glucose, (ii) have a low glycaemic index, (iii) increases the bioavailability of iron by forming an iron‐chelate complex, (iv) accelerates ethanol metabolism, etc.…”

Section: Introductionmentioning

confidence: 99%

Functional characterization and in vitro screening of Fructobacillus fructosus MCC 3996 isolated from Butea monosperma flower for probiotic potential

Patil

Jadhav²,

Patil³

2020

Lett Appl Microbiol

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The fructophilic bacterium Fructobacillus fructosus MCC 3996 described in the present investigation was isolated from the nectar of Butea monosperma flower and evaluated in vitro for the manifestation of probiotic features. The strain utilizes fructose faster than glucose and is capable to grow in the range of 1–35% fructose concentration (optimum 5% w/v) and thus denotes its fructophilic nature. In vitro assessments of the strain have examined for the endurance in acidic environment/gastric juice, the better auto‐aggregation ability even in the presence of hydrolytic enzymes, co‐aggregation with pathogenic bacteria, hydrophobicity properties and no haemolytic activity to elucidate its feasible probiotic use. The significant antagonistic activity against several detrimental bacteria, despite lacking the bacteriocin secretion, is an astonishing feature. Owing to the indigenous origin of the isolate, it could be used as a probiotic, starter culture, and/or the active ingredient of food formulation may contribute to improve the desirable fermentation, long‐term storage and nutritional benefits of foods especially rich in fructose. Significance and Impact of the Study This study provided in vitro evidence that Fructobacillus fructosus MCC 3996 have endurance in acidic gastric juice, better co‐aggregation, auto‐aggregation properties, splendid antagonistic activities against several bacteria involved in food spoilage/human infections, pertinent antibiotic susceptibility profile and no haemolytic activity. Also, F. fructosus have the capability to survive in the appreciable amount of fructose, and this advocates that the strain could be used as starter culture and/or the active ingredient of fructose‐rich foods. The current in vitro study provided a strong basis for further in vivo research to identify the health beneficial characteristics of F. fructosus and its potential could be effectively utilized as health‐boosting ingredient in food and pharmaceutical industries.

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RETRACTED: Biocatalytic strategies for the production of high fructose syrup from inulin

Cited by 65 publications

References 81 publications

Identification and Immobilization of an Invertase With High Specific Activity and Sucrose Tolerance Ability of Gongronella sp. w5 for High Fructose Syrup Preparation

Identification and Immobilization of an Invertase With High Specific Activity and Sucrose Tolerance Ability of Gongronella sp. w5 for High Fructose Syrup Preparation

Biodegradable super porous inulin cryogels as potential drug carrier

Functional characterization and in vitro screening of Fructobacillus fructosus MCC 3996 isolated from Butea monosperma flower for probiotic potential

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