In this paper, we present the optimization of green tea
leaf (Camellia sinensis L.) extraction,
carried out using
water and hydroalcoholic solvents, for the subsequent synthesis of
silver nanoparticles (AgNPs). The value ranges for independent variables,
including pH, time, and temperature, were selected based on single-factor
experiments and used for extraction in the order presented by the
Box–Behnken design. Three-dimensional response surface graphs
were used to visually present the optimization results and determine
the optimal extraction conditions: pH = 7, 30 min, 80 °C for
water and pH = 5.5, 50 min, and 80 °C for water–ethanol.
Our findings indicate that the water–ethanol mixture extracted
more polyphenols. We compared the physicochemical properties of AgNPs
obtained using both types of extractants via DLS and TEM analysis.
We proposed a predicted mechanism for the reduction and stabilization
of AgNPs based on the Fourier transform infrared data. The hydroethanolic
extract leads to significant nanoparticle aggregation, which can be
explained by the nucleation theory and agglomeration of nanoparticles
in the presence of excess macromolecular organic substances (flocculation).