RETRACTED: Circular RNA circ-FoxO3 Inhibits Myoblast Cells Differentiation

Abstract

CircRNA is a type of closed circular non-coding RNA formed by reverse splicing and plays an important role in regulating the growth and development of plants and animals. To investigate the function of circ-FoxO3 in mouse myoblast cells’ (C2C12) differentiation and proliferation, we used RT-qPCR to detect the expression level of circ-FoxO3 in mouse myoblast cells at different densities and different differentiation stages, and the specific interference fragment was used to inhibit the expression level of circ-FoxO3 in myoblast cells to observe its effect on myoblast cells proliferation and differentiation. We found that the expression level of circ-FoxO3 in myoblast cells increased with the prolongation of myoblast cells differentiation time, and its expression level decreased with the proliferation of myoblast cells. At the same time, we found that the differentiation ability of the cells was significantly increased (p < 0.05), but the cell proliferation was unchanged (p > 0.05) after inhibiting the expression of circ-FoxO3 in myoblast cells. Combining the results of bioinformatics analysis and the dual luciferase reporter experiment, we found that circ-FoxO3 is a sponge of miR-138-5p, which regulates muscle differentiation. Our study shows that circ-FoxO3 can inhibit the differentiation of C2C12 myoblast cells and lay a scientific foundation for further study of skeletal muscle development at circRNA levels.

circRNAs expressed in human peripheral blood are associated with human aging phenotypes, cellular senescence and mouse lifespan

“…It is not clear whether the previously reported circular FOXO3 transcripts have the same structure as the one we have identified, since previous studies do not give its exon structure. A circRNA from the FOXO3 gene identical to the one we have identified has also previously been demonstrated to inhibit myoblast differentiation in mouse cells (Li et al 2019). Genetic variation in the FOXO3 gene itself has previously been associated with extreme longevity (Flachsbart et al 2017;Fuku et al 2016) and has also been associated with maintenance of telomere length (Davy et al 2018).…”

A Novel Nanobody Directed against Ovine Myostatin to Enhance Muscle Growth in Mouse

“…MSTN belongs to the TGF-β superfamily and can negatively control muscle growth. Our laboratory has contributed to develop a safe and effective strategy to enhance animal growth and meat production for a long time [ 11 , 12 , 24 ]. Various studies have shown that inhibiting MSTN activity can effectively promote the growth of animal muscles [ 25 , 26 ].…”

Functions of Circular RNAs in Regulating Adipogenesis of Mesenchymal Stem Cells

“…In skeletal muscle satellite cells (SMSCs), it subsequently activates myogenesis through sponging miR-7 [70,71]. Several circRNAs also work in the same way by sponging miRNAs to promote or repress the myogenic differentiation of MSCs, such as circHIPK3 and circ-FoxO3 [72][73][74]. Other than that, cir-cRNAs have been identified to be differentially expressed in different differentiation stages of neural stem cells (NSCs).…”