RETRACTED: Cucumber PDR8/ABCG36 and PDR12/ABCG40 plasma membrane proteins and their up-regulation under abiotic stresses

Migocka, Magdalena; Papierniak, Anna; Rajsz, Adam

doi:10.1007/s10535-016-0679-2

Cited by 12 publications

(6 citation statements)

References 34 publications

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“…PDR1 (XP_015937455.1) is orthologous to AtPDR12/ABCG40, which is a plasma membrane transporter that functions as an efflux pump for Pb 2+ exclusion in Arabidopsis [30]. In cucumber, CsPDR12/CsABCG40 was up-regulated under Cd stress [31]. Thus, the up-regulation of these transporters by Cd exposure in Silihong might enhance Cd detoxification by increasing its vacuolar sequestration or efflux from symplast to apoplast.…”

Section: Discussionmentioning

confidence: 99%

Comparative proteomics analysis of peanut roots reveals differential mechanisms of cadmium detoxification and translocation between two cultivars differing in cadmium accumulation

Jiang

et al. 2019

BMC Plant Biol

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Background Peanut is one of the most important oil and protein crops, and it exhibits wide cultivar variations in shoot Cd accumulation ability. However, the mechanism of Cd accumulation in peanut shoots has not been well understood. In this study, the root proteomics of two cultivars differing in seed Cd accumulation, Fenghua 1 (F, low Cd cultivar) and Silihong (S, high Cd cultivar), were investigated under 0 (CK) and 2 μM Cd conditions. Results A total of 4676 proteins were identified by proteomics screening. Of them, 375, 1762, 1276 and 771 proteins were identified to be differentially expressed proteins (DEPs) for comparison of F Cd /F CK , S Cd /S CK , F CK /S CK and F Cd /S Cd , respectively. Silihong is more sensitive to Cd exposure than Fenghua 1 in terms of root proteomics. A total of 30 and 86 DEPs were identified to be related with heavy metal transport and cell wall modification, respectively. The up-regulation of ABCB25, ABCC14, ABCC2, PDR1 and V-ATPases by Cd exposure in Silihong might enhance vacuolar sequestration of Cd and its efflux from symplast to apoplast. The higher Cd accumulation in the root CWs of Silihong might be resulted from its higher capability of CW modification, in which many proteins such as IRX10L, BGLU12-like, BGLU42, EXLB1, XTH30, XTH6, XYL7, PAL3, COMT, CAD1, and CCR1 were involved. Conclusions The vacuolar sequestration and efflux of Cd as well as its adsorption in CW might be the principal mechanism of cadmium detoxification in Silihong. The higher capacity of Cd accumulation and translocation of Silihong is an inherent characteristics in which ACA8 and ZIP1 might be involved. Electronic supplementary material The online version of this article (10.1186/s12870-019-1739-5) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Comparative proteomics analysis of peanut roots reveals differential mechanisms of cadmium detoxification and translocation between two cultivars differing in cadmium accumulation

Jiang

et al. 2019

BMC Plant Biol

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“…AtABCG36/PDR8, localized at the plasma membrane, plays an important role in Cd extrusion from root cells [18]. The transcription of cucumber genes CsPDR8/CsABCG36 can be up-regulated under Cd stress [19]. The rice OsABCG43/PDR5 is a Cd inducible-transporter and confers high Cd resistance in yeast cells [20].…”

Section: Introductionmentioning

confidence: 99%

Ectopic Expression of Poplar ABC Transporter PtoABCG36 Confers Cd Tolerance in Arabidopsis thaliana

Wang

Liu

Peng

et al. 2019

IJMS

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Cadmium (Cd) is one of the most toxic heavy metals for plant growth in soil. ATP-binding cassette (ABC) transporters play important roles in biotic and abiotic stresses. However, few ABC transporters have been characterized in poplar. In this study, we isolated an ABC transporter gene PtoABCG36 from Populus tomentosa. The PtoABCG36 transcript can be detected in leaves, stems and roots, and the expression in the root was 3.8 and 2 times that in stems and leaves, respectively. The PtoABCG36 expression was induced and peaked at 12 h after exposure to Cd stress. Transient expression of PtoABCG36 in tobacco showed that PtoABCG36 is localized at the plasma membrane. When overexpressed in yeast and Arabidopsis, PtoABCG36 could decrease Cd accumulation and confer higher Cd tolerance in transgenic lines than in wild-type (WT) lines. Net Cd2+ efflux measurements showed a decreasing Cd uptake in transgenic Arabidopsis roots than WT. These results demonstrated that PtoABCG36 functions as a cadmium extrusion pump participating in enhancing tolerance to Cd through decreasing Cd content in plants, which provides a promising way for making heavy metal tolerant poplar by manipulating ABC transporters in cadmium polluted areas.

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“…We also identified some DEGs encoding plasma membrane-localized transporter proteins related to Cd efflux, including ABCG36/PDR8, PDR1, YSL3/7, MTP9, DTX43 and ZIP1/5 (Table 1). These genes have been reported to regulate in Cd accumulation or detoxification in other plants [38–41]. Migocka et al [38] reported that CsMTP9 in cucumber was a plasma membrane H + -coupled antiporter involved in the Mn 2+ and Cd 2+ efflux from root cells.…”

Section: Discussionmentioning

confidence: 99%

Comparative transcriptome analysis revealed key factors for differential cadmium transport and retention in roots of two contrasting peanut cultivars

et al. 2018

BMC Genomics

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BackgroundPeanut is the world’s fourth largest oilseed crop that exhibits wide cultivar variations in cadmium (Cd) accumulation. To establish the mechanisms of Cd distribution and accumulation in peanut plants, eight cDNA libraries from the roots of two contrasting cultivars, Fenghua 1 (low-Cd cultivar) and Silihong (high-Cd cultivar), were constructed and sequenced by RNA-sequencing. The expression patterns of 16 candidate DEGs were validated by RT-qPCR analysis.ResultsA total of 75,634 genes including 71,349 known genes and 4484 novel genes were identified in eight cDNA libraries, among which 6798 genes were found to be Cd-responsive DEGs and/or DEGs between these two cultivars. Interestingly, 183 DEGs encoding ion transport related proteins and 260 DEGs encoding cell wall related proteins were identified. Among these DEGs, nine metal transporter genes (PDR1, ABCC4 and ABCC15, IRT1, ZIP1, ZIP11, YSL7, DTX43 and MTP4) and nine cell wall related genes (PEs, PGIPs, GTs, XYT12 CYP450s, LACs, 4CL2, C4H and CASP5) showed higher expression in Fenghua 1 than in Silihong.ConclusionsBoth the metal transporters and cell wall modification might be responsible for the difference in Cd accumulation and translocation between Fenghua 1 and Silihong. These findings would be useful for further functional analysis, and reveal the molecular mechanism responsible for genotype difference in Cd accumulation.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-5304-7) contains supplementary material, which is available to authorized users.

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RETRACTED: Cucumber PDR8/ABCG36 and PDR12/ABCG40 plasma membrane proteins and their up-regulation under abiotic stresses

Cited by 12 publications

References 34 publications

Comparative proteomics analysis of peanut roots reveals differential mechanisms of cadmium detoxification and translocation between two cultivars differing in cadmium accumulation

Comparative proteomics analysis of peanut roots reveals differential mechanisms of cadmium detoxification and translocation between two cultivars differing in cadmium accumulation

Ectopic Expression of Poplar ABC Transporter PtoABCG36 Confers Cd Tolerance in Arabidopsis thaliana

Comparative transcriptome analysis revealed key factors for differential cadmium transport and retention in roots of two contrasting peanut cultivars

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