RETRACTED: Development and validation of an LC–MS method with electrospray ionization for quantitation of digoxin in human plasma and urine: Application to a pharmacokinetic study

Shi, Shaojun; Li, Zhongfang; Chen, Huating; Fan-dian, Zeng

doi:10.1016/j.jchromb.2008.09.016

Cited by 5 publications

(1 citation statement)

References 15 publications

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“…First done literature survey related to new Digoxin tablet formulation, solvent selection, method validation and result analysis. Several reports have described the methods for quantifying Digoxin in different samples and applying various modes of detections such as liquid chromatography-tandem mass spectrometry [4][5][6][7] , LC-MS / MS analysis of Digoxin 8 , RP-HPLC method for β-acetyldigoxin 9 , pulsed amperometric detection 10 and liquid chromatography with ultraviolet detection 11 . The official Pharmacopoeia present monographs of Digoxin raw material, tablets, oral solution and injection 12 , 13 .…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Estimation of Drug Digoxin in Tablet Dosage Form by UV Spectrophotometric Method

Paulzagade¹,

Borkar²

2021

Int J Life Sci Pharm Res

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Digoxin has a cardiac glycoside property. Aim of the present study was to develop UV -Spectrophotometric method using simultaneous equation for the drug Digoxin in tablet formulation. Objective of the study is to develop a simple and precise analytical method for the drug Digoxinwith statistical data. In validation, our study includes accuracy, precision, specificity, limit of detection, limit of quantitation, linearity and range parameters. Digoxin has antihypertensive activity and various new tablet formulations were already introduced in the market, so we compared the results of marketed tablet formulation with standard drug Digoxin. Literature review helped us in planning of work that includes selection of solvent, wavelength identification, sample preparation, analyzing test & standard solutions and validation study. The detection of the drug was carried out in 220 nm. The method was linear (Correlation Coefficient= 0.99) over the range of 25 to 125 µg/ml, precise (Standard Deviation = 0-1, Relative Standard Deviation < 2%), accurate (mean recoveries from 97.5% and 104.3%), and had a LOD and LOQ equal to 0.12 and 0.38 µg/ml, respectively. For specific stability, the forced degradation study (alkali, acid, oxide and heat) was performed on marketed formulations to show the stability indicating ability of the developed method. The method showed robustness, remaining unaffected by deliberated variations in spectrophotometric conditions. Due to correlative results the validated analytical method was successfully applied for the quantification of Digoxin and demonstrated the uniform distribution of the drug into the systems. Finally, we developed an accurate, less time consuming and cheaper UV-Spectrophotometric method for the determination of the Digoxin in tablet formulation.

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Section: Introductionmentioning

confidence: 99%

Simultaneous Estimation of Drug Digoxin in Tablet Dosage Form by UV Spectrophotometric Method

Paulzagade¹,

Borkar²

2021

Int J Life Sci Pharm Res

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Current literature in mass spectrometry

2009

J. Mass Spectrom.

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In order to keep subscribers up‐to‐date with the latest developments in their field, John Wiley & Sons are providing a current awareness service in each issue of the journal. The bibliography contains newly published material in the field of mass spectrometry. Each bibliography is divided into 11 sections: 1 Reviews; 2 Instrumental Techniques & Methods; 3 Gas Phase Ion Chemistry; 4 Biology/Biochemistry: Amino Acids, Peptides & Proteins; Carbohydrates; Lipids; Nucleic Acids; 5 Pharmacology/Toxicology; 6 Natural Products; 7 Analysis of Organic Compounds; 8 Analysis of Inorganics/Organometallics; 9 Surface Analysis; 10 Environmental Analysis; 11 Elemental Analysis. Within each section, articles are listed in alphabetical order with respect to author

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A rapid chromatographic/mass spectrometric method for digoxin quantification in human plasma

et al. 2009

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The relatively simple liquid-liquid extraction LC-MS/MS method using a UPLC column for measurement of digoxin concentrations both in medium and human plasma is described. Digitoxin was used as internal standard. The developed method possesses satisfactory accuracy, precision, and repeatability, and is economical and not time-consuming. It is the first method for precise measurement of digoxin concentrations in human plasma using combined HPLC equipment and UPLC column with a low limit of quantitation equal to 0.1 ng ml -1 as verified in more than 1500 samples analyzed in a GCP GLP bioequivalence study. The developed method was successfully used in digoxin bioequivalence studies in which 26 volunteers were enrolled.

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RETRACTED: Development and validation of an LC–MS method with electrospray ionization for quantitation of digoxin in human plasma and urine: Application to a pharmacokinetic study

Cited by 5 publications

References 15 publications

Simultaneous Estimation of Drug Digoxin in Tablet Dosage Form by UV Spectrophotometric Method

Simultaneous Estimation of Drug Digoxin in Tablet Dosage Form by UV Spectrophotometric Method

Current literature in mass spectrometry

A rapid chromatographic/mass spectrometric method for digoxin quantification in human plasma

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