RETRACTED: Evaluation of antioxidant activities, toxicity studies and the DNA damage protective effect of various solvent extracts of Litsea cubeba fruits

Abstract

Litsea cubeba is devoured by the ethnic individuals of Arunachal Pradesh in India as food and has been traditionally used for curing different ailments. The purpose of present study was to investigate the antioxidant activities of fruits of L. cubeba using different solvent extracts, quantification of phenolics, toxicity studies and DNA damage protective activities. The antioxidant activities of fruits using five different solvent extracts completed utilizing different in vitro examines. The quantitation of phenolic and polyphenolic compounds in the methanol extract of the fruits was carried out by HPLC. The in vitro haemolytic examination of plant concentrates were completed on rat erythrocytes. Appraisal of cytotoxicity of eatable fruits was assessed by MTT measure. The genotoxicity of the contemplated plant was tried by the single-cell gel electrophoresis comet measure. The DNA defensive impacts of the aqueous extracts of fruits on rodent lymphocyte DNA lesions were likewise assessed with the comet test. The extract obtained by methanol exhibited the highest antioxidant activity. The HPLC examination of the methanol concentrate of the plant demonstrated the occurrence of different phenolic acids and flavonoids like caffeic acid (145.96μg/100mg DE), syringic acid (125.85 μg/100mg DE), ferulic acid (155.89 μg/ 100mg DE), apigenin (28.43 μg/100mg DE), kaempferol (53.41 μg/100mg DE) etc. in various amounts. The consequences of haemolytic lethality, cytotoxicity and genotoxicity of fluid concentrates of the edible plant ensure the security at cell and genomic level. The fluid concentrate of the plant fundamentally repressed DNA harm and these information recommend that the watery concentrate of L. cubeba can forestall oxidative DNA harm to rodent lymphocytes, which is likely because of antioxidant constituents in the concentrate. These outcomes demonstrate that L. cubeba can be utilized in dietary applications with a possibility to diminish oxidative pressure.

Effect of combined treatment including Litsea cubeba essential oil and wood smoke on Aspergillus niger development and consumer acceptability of dried catfish (Ariidae)

“…These antioxidants act as radical scavengers and are capable of radical scavenging, recombining radicals, chelating the transition of metal ions and transferring electrons, with the formation of stable food products as the result of those activities. LC has been reported to have high antioxidant activity, similar to chemical antioxidants (Seal et al., 2020). Citral has been shown to be the major component responsible for ferric reducing antioxidant potential and important antioxidant activities (Bouzenna et al., 2017).…”

Ameliorating Effect on Aβ-Induced Alzheimer’s Mice by Litsea cubeba Persoon Powder

“…These compounds demonstrated significant antioxidant, radical-scavenging and protective effects in β-amyloid-induced neurotoxicity [ 40 ]. The high levels of phenolic acids and flavonoid compounds further demonstrated the antioxidant, hemolytic lethality, and cytotoxicity activities of L. cubeba fruit extracts [ 41 ]. Based on the above findings, it could be postulated that LCP was utilized in dietary applications to reduce oxidative pressure and related damage in in vivo and in vitro studies.…”

Effects of Litsea cubeba Essential Oil–Chitosan/Corn Starch Composite Films on the Quality and Shelf-Life of Strawberry (Fragaria × ananassa)

“…For fruits stored at 4 • C (Figure 3C), the accumulation of MDA was much slower, and after only 18 d of storage, its concentration reached 10.74 and 9.86 µmoL/g for CH/CS/gly film-coated fruits and LCEO/CH/CS/gly film-coated fruits, respectively. The much slower accumulation of MDA was most likely due to the incorporation of LCEO in the coating mixture, as it is well documented that LCEO, like most EOs, has strong antioxidant activities [40], which would retard the oxidation of the lipids in the cell membrane.…”