RETRACTED: Genetic polymorphism and phylogenetic analyses of 21 non‐CODIS STR loci in a Chinese Han population from Shanghai

Zhou, Zhihan; Shao, Chengchen; Xie, Jianhui; Xu, Hongmei; Liu, Yidong; Zhou, Yueqin; Liu, Zhiping; Zhao, Ziqin; Tang, Qiqun; Sun, Kuan

doi:10.1002/mgg3.1083

Cited by 6 publications

(2 citation statements)

References 30 publications

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“…Given the utility of STR-based approaches to answer questions related to wildlife crime investigations as forensic genetics, efforts should be devoted to the characterization of microsatellite primers for species threatened by illegal trafficking. However, while in human forensics, the selection of around 20 core STR loci allowed the standardization around the globe for human identity testing [43,44], accomplishing this same achievement is more challenging for the hyper-diverse animal assemblage encountered in wildlife forensics [38].…”

Section: Discussionmentioning

confidence: 99%

Cross-Amplification in Strigiformes: A New STR Panel for Forensic Purposes

Giangregorio

Naldi

Mengoni

et al. 2021

Genes

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Strigiformes are affected by a substantial decline mainly caused by habitat loss and destruction, poaching, and trapping. Moreover, the increasing trend in bird trade and the growing interest in wild-caught rather than captive-bred birds are expected to encourage illegal trade. The biomolecular investigation represents a valuable tool to track illegal trade and to explore the genetic variability to preserving biodiversity. Microsatellite loci (STRs) are the most used markers to study genetic variability. Despite the availability of species-specific microsatellite loci in Strigiformes, a unique panel permitting the description of the genetic variability across species has not been identified yet. We tested 32 highly polymorphic microsatellite markers to evaluate the reliability of a unique microsatellite panel in different species of Strigiformes and its use for conservation and forensic purposes. We included in the study 84 individuals belonging to 28 parental groups and 11 species of Strigiformes. After screening polymorphic microsatellite loci, the description of genetic variability, and the kinship assessment, we characterized a final panel of 12 microsatellite loci able to identify individuals in 9 Strigiformes species. This STR panel might support the authorities in the forensic investigation for suspected smugglers and false parental claims; moreover, it can be useful to evaluate relatedness among individuals in captive-bred populations and to implement research projects finalized to the description of the genetic variability in wild populations.

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Section: Discussionmentioning

confidence: 99%

Cross-Amplification in Strigiformes: A New STR Panel for Forensic Purposes

Giangregorio

Naldi

Mengoni

et al. 2021

Genes

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“…12 STR loci is sufficient for paternity testing [30,31]. Therefore, given the limitations of CE and SNP-based NGS, mini-STR-based NGS with high testing capacity and high PIC is a better choice for NIPPT.…”

Section: Plos Onementioning

confidence: 99%

Non-invasive prenatal paternity testing by analysis of Y-chromosome mini-STR haplotype using next-generation sequencing

Song¹,

Nan²,

Zhou³

et al. 2022

PLoS ONE

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Objectives To assess the efficacy of Y-chromosome mini-STR-based next-generation sequencing (NGS) for non-invasive prenatal paternity testing (NIPPT). Methods DNA was extracted from the plasma of 24 pregnant women, and cell-free fetal DNA (cffDNA) haplotyping was performed at 12 Y-chromosome mini-STR loci using the Illumina NextSeq 500 system. The cffDNA haplotype was validated by the paternal haplotype. Subsequentlly, the paternity testing parameters were attributed to each case quantitatively. Results The biological relationship between the alleged fathers and infants in all 24 family cases were confirmed by capillary electrophoresis (CE). The Y-chromosome mini-STR haplotypes of all 14 male cffDNA were obtained by NGS without any missing loci. The alleles of cffDNA and paternal genomic DNA were matched in 13 cases, and a mismatched allele was detected at the DYS393 locus in one case and considered as mutation. No allele was detected in the 10 female cffDNA. The combined paternity index (CPI) and probability of paternity calculation was based on 6 loci Y-haplotype distributions of a local population. The probability of paternity was 98.2699–99.8828% for the cases without mutation, and 14.8719% for the case harboring mutation. Conclusions Our proof-of-concept study demonstrated that Y-chromosome mini-STR can be used for NGS-based NIPPT with high accuracy in real cases, and is a promising tool for familial searching, paternity exclusion and sex selection in forensic and medical applications.

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