Retracted: In vitro genotoxicity assessment of nickel(II) oxide nanoparticles on lymphocytes of human peripheral blood

Dumala, Naresh; Mangalampalli, Bhanuramya; Grover, Paramjit

doi:10.1002/jat.3784

Cited by 8 publications

(2 citation statements)

References 48 publications

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“…Immune cells. The work [45] concentrated on studying NiO cytotoxicity in lymphocytes extracted from human peripheral blood. Primary NPs size was 18 nm; they aggregated intensely in water media.…”

Section: Cytotoxicity Of Ni-containing Nanomaterialsmentioning

confidence: 99%

Assessing risks caused by nickel-based nanomaterials: hazardous factor identification

Gmoshinski¹,

lane²,

Хотимченко³

2021

Health Risk Analysis

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Nanoparticles of nickel (Ni) and its compounds attract a lot of attention bearing in mind their promising innovative properties allowing their use as catalysts, components in electrical appliances, electronic devices and photonic appliances, and materials used in producing medications, diagnostic preparations, and pesticides. Production volumes of these materials in their nano-form are likely to grow rapidly in the nearest future and it involves greater loads created by these nanomaterials on a human body. And we should remember that Ni and its compounds are highly toxic for humans even in their traditional disperse forms. Their toxicity induces oxidative stress, cellular membranes and mitochondria dysfunction, expression of nuclear transcription factors that are responsible for apoptosis, caspases, as well as proto-oncogenes. Leading role in toxicity of Ni-containing nanomaterials obviously belongs to ions of heavy Ni++ being emitted from them since this heavy metal has pro-oxidant properties and influences enzyme activity and gene expression. Cytotoxic effects produced by Ni-containing nanomaterials were revealed in Model experiments in vitro performed with suing cellular cultures that were morphologically and functionally similar to epithelial cells of respiratory and gastrointestinal tract, liver, kidneys, and nervous system; these materials were able to stimulate oxidant stress, influence expression of apoptosis proteins and nuclear transcription factors, induce apoptosis and necrosis. There are data indicating that Ni-containing nanomaterials can produce malignant transforming effects in vitro. All the above mentioned proves that nickel compounds in their nanoform are a new hazardous factor that requires assessing related risks for workers, consumer, and population in general. Our review focuses on analyzing literature sources on cytotoxicity of Ni-containing nanomaterials and their effects produced on molecular-genetic and cellular levels taken over a period starting from 2011.

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Section: Cytotoxicity Of Ni-containing Nanomaterialsmentioning

confidence: 99%

Assessing risks caused by nickel-based nanomaterials: hazardous factor identification

Gmoshinski¹,

lane²,

Хотимченко³

2021

Health Risk Analysis

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“…However, their extensive production and usage lead to a demand for toxicity evaluation as well as assessment of health risks at occupational and environmental settings ( Kessler, 2011 ; Batley et al, 2013 ). Among the various NPs, nickel oxide (NiO) is vastly employed in industrial applications such as metal printing, electronics, ceramics, catalysis, and sensing ( Zhou et al, 2017 ; Sousa et al, 2018 ; Dumala et al, 2019 ; Jaji et al, 2020 ; Taeño et al, 2021 ; Wang et al, 2021 ). Moreover, exposure is common at nickel refineries, metal alloy production sites and at occupational setting where welding is performed ( Klein and Costa, 2015 ; Pesch et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Primary and Secondary Genotoxicity of Nanoparticles: Establishing a Co-Culture Protocol for Assessing Micronucleus Using Flow Cytometry

Vallabani

Karlsson

2022

Front. Toxicol.

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Genotoxicity is an important endpoint to assess for understanding the risks associated with nanoparticles (NPs). Most genotoxicity studies performed on NPs have focused on primary genotoxicity analyzed by comet- or micronuclei (MN) assay using microscopic scoring. Here, we established a protocol for a more efficient version of MN assessment using flow cytometry and, importantly, both primary and secondary (inflammation-driven) genotoxicity was assessed. Human bronchial epithelial cells (HBEC-3kt) were exposed to nickel oxide (NiO) NPs directly or indirectly. The indirect exposure was done to assess secondary genotoxicity, and in this case immune cells (THP-1 derived macrophages) were exposed on inserts and the HBEC were cultured in the lower compartment. The results in monocultures showed that no increased MN formation was observed in the HBEC cells but instead a clear MN induction was noted in THP-1 cells indicating higher sensitivity. No MN formation was either observed when the HBEC were indirectly exposed, but an increase in DNA strand breaks was detected using the comet assay. Taken together, the present study emphasizes the feasibility of assessing primary and secondary genotoxicity and, furthermore, shows a clear MN induction in THP-1 monoculture following NiO NPs exposure.

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Genotoxicity Evaluation of Nanosized Materials

Sangeetha

Arun

Mohanan

2023

Biomedical Applications and Toxicity of Nanomaterials

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Retracted: In vitro genotoxicity assessment of nickel(II) oxide nanoparticles on lymphocytes of human peripheral blood

Cited by 8 publications

References 48 publications

Assessing risks caused by nickel-based nanomaterials: hazardous factor identification

Assessing risks caused by nickel-based nanomaterials: hazardous factor identification

Primary and Secondary Genotoxicity of Nanoparticles: Establishing a Co-Culture Protocol for Assessing Micronucleus Using Flow Cytometry

Genotoxicity Evaluation of Nanosized Materials

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