RETRACTED: LINC00174 Facilitates Cell Proliferation, Cell Migration and Tumor Growth of Osteosarcoma via Regulating the TGF-β/SMAD Signaling Pathway and Upregulating SSH2 Expression

Zheng, Changjun; Li, Ronghang; Zheng, Shuang; Fang, Hongjuan; Xu, Meng; Zhong, Lei

doi:10.3389/fmolb.2021.697773

Cited by 9 publications

(4 citation statements)

References 44 publications

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“…circ-RANGAP1 and MYLIP expressions were normalized to GAPDH while miR-542-3p expression was to U6. The 2 - ΔΔ Ct method was utilized to calculate gene expression, and the primer sequence was presented in Table 1 [ 13 ].…”

Section: Methodsmentioning

confidence: 99%

circ-RANGAP1/MicroRNA-542-3p/Myosin Regulatory Light Chain Interacting Protein Axis Modulates the Osteosarcoma Cell Progression

Sheng

Liu

et al. 2022

Applied Bionics and Biomechanics

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Objective. This study is aimed at exploring the influence of circular RNA- (circRNA-) RANGAP1 targeting microRNA- (miR-) 542-3p/myosin regulatory light chain interacting protein (MYLIP) on the biological function of osteosarcoma (OS) cells. Methods. Tumor tissues and normal tissues were collected from OS patients and circ-RANGAP1, miR-542-3p, and MYLIP expression was tested by RT-qPCR. The correlation between the clinicopathology/prognosis of patients with OS and circ-RANGAP1 expression was observed. Human OS cell line MG-63 was screened to determine the influences of circ-RANGAP1 and miR-542-3p on OS cell progression. The targeting relation of circ-RANGAP1, miR-542-3p, and MYLIP was probed. Results. circ-RANGAP1 expression was elevated in tumor tissues from OS patients, which was correlated to the poor clinicopathology. circ-RANGAP1 expression was augmented in males or patients younger than 20 years old or patients with advanced OS. Higher circ-RANGAP1 expression indicated a poor prognosis in OS patients. After silencing circ-RANGAP1 or elevating miR-542-3p in MG63 cells, cell progression was limited. miR-542-3p downregulation reduced the therapeutic efficacy of silenced circ-RANGAP1. circ-RANGAP1 bound with miR-542-3p to target MYLIP. Conclusion. Silenced circ-RANGAP1 boosts MYLIP expression via competitive binding of miR-542-3p to facilitate OS cell progression.

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Section: Methodsmentioning

confidence: 99%

circ-RANGAP1/MicroRNA-542-3p/Myosin Regulatory Light Chain Interacting Protein Axis Modulates the Osteosarcoma Cell Progression

Sheng

Liu

et al. 2022

Applied Bionics and Biomechanics

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“…One of the five mitophagy-related lncRNAs, LINC00174, is an lncRNA whose high expression was associated with poor prognosis in our risk model. LINC00174 plays an oncogenic role in several cancers, including colorectal cancer [ 34 , 35 ], renal clear cell carcinoma [ 36 ], hepatocellular carcinoma [ 37 ], breast cancer [ 38 ], glioma [ 39 – 42 ], osteosarcoma [ 43 ], thymic epithelial tumors [ 44 ], and lung cancer [ 45 , 46 ]. Contrary to these conclusions, Cheng et al suggested that LINC00174 acts as a tumor suppressor gene in non-small cell lung cancer, and overexpression of LINC00174 inhibits NSCLC cell migration and proliferation, and induces apoptosis [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

Mitophagy-related long non-coding RNA signature predicts prognosis and drug response in Ovarian Cancer

Wang,

Zhang,

Zheng

et al. 2023

J Ovarian Res

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Background Ovarian cancer (OC) is the most malignant tumor with the worst prognosis in female reproductive system. Mitophagy and long non-coding RNAs (lncRNAs) play pivotal roles in tumorigenesis, development, and drug resistance. The effects of mitophagy-related lncRNAs on OC prognosis and therapeutic response remain unelucidated. Methods We retrieved OC-related RNA sequence, copy number variation, somatic mutation, and clinicopathological information from The Cancer Genome Atlas database and mitophagy-related gene sets from the Reactome database. Pearson’s correlation analysis was used to distinguish mitophagy-related lncRNAs. A prognostic lncRNA signature was constructed using UniCox, LASSO, and forward stepwise regression analysis. Individuals with a risk score above or below the median were classified as high- or low-risk groups, respectively. The risk model was analyzed using the Kaplan–Meier estimator, receiver operating characteristic curve, decision curve analysis, and Cox regression analysis and validated using an internal dataset. LINC00174 was validated in clinical samples and OC cell lines. We also reviewed reports on the role of LINC00174 in cancer. Subsequently, a nomogram model was constructed. Furthermore, the Genomics of Drug Sensitivity in Cancer database was used to explore the relationship between the risk model and anti-tumor drug sensitivity. Gene set variation analysis was performed to assess potential differences in biological functions between the two groups. Finally, a lncRNA prognostic signature-related competing endogenous RNA (ceRNA) network was constructed. Results The prognostic signature showed that patients in the high-risk group had a poorer prognosis. The nomogram exhibited satisfactory accuracy and predictive potential. LINC00174 mainly acts as an oncogene in cancer and is upregulated in OC; its knockdown inhibited the proliferation and migration, and promoted apoptosis of OC cells. High-risk patients were more insensitive to cisplatin and olaparib than low-risk patients. The ceRNA network may help explore the potential regulatory mechanisms of lncRNAs. Conclusion The mitophagy-related lncRNA signature can help estimate the survival and drug sensitivity, the ceRNA network may provide novel therapeutic targets for patients with OC.

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“…In osteosarcoma (OS), high levels of lnc00174 form a ceRNA network with miR-378a-3p/SSH2 and activate the TGF-β/SMAD pathway to promote OS cell proliferation ( 121 ).…”

Section: Tgf-β Pathway-related Lncrnas and Other Cancer Typesmentioning

confidence: 99%

Roles of TGF‑β signalling pathway‑related lncRNAs in cancer (Review)

Liu

et al. 2023

Oncol Lett

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Long non-coding RNAs (lncRNAs) are a class of RNAs that are >200 nucleotides in length that do not have the ability to be translated into protein but are associated with numerous diseases, including cancer. The involvement of lncRNAs in the signalling of certain signalling pathways can promote tumour progression; these pathways include the transforming growth factor (TGF)-β signalling pathway, which is related to tumour development. The expression of lncRNAs in various tumour tissues is specific, and their interaction with the TGF-β signalling pathway indicates that they may serve as new tumour markers and therapeutic targets. The present review summarized the role of TGF-β pathway-associated lncRNAs in regulating tumorigenesis in different types of cancer and their effects on the TGF-β signalling pathway.

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RETRACTED: LINC00174 Facilitates Cell Proliferation, Cell Migration and Tumor Growth of Osteosarcoma via Regulating the TGF-β/SMAD Signaling Pathway and Upregulating SSH2 Expression

Cited by 9 publications

References 44 publications

circ-RANGAP1/MicroRNA-542-3p/Myosin Regulatory Light Chain Interacting Protein Axis Modulates the Osteosarcoma Cell Progression

circ-RANGAP1/MicroRNA-542-3p/Myosin Regulatory Light Chain Interacting Protein Axis Modulates the Osteosarcoma Cell Progression

Mitophagy-related long non-coding RNA signature predicts prognosis and drug response in Ovarian Cancer

Roles of TGF‑β signalling pathway‑related lncRNAs in cancer (Review)

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