RETRACTED: MicroRNA-138 modulates metastasis and EMT in breast cancer cells by targeting vimentin

Zhang, Jun; Liu, Dan; Feng, Zhuo; Murata, Jun; Zhang, Chunying; Lü, Ying; Li, Jiazhi; Zhang, Qingqing; Li, Qing; Li, Lianhong

doi:10.1016/j.biopha.2015.12.018

Cited by 99 publications

(65 citation statements)

References 20 publications

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“…In addition, the overall survival of NSCLC patients with low miR-138 expression was significantly shorter than those with high miR-138 expression (27). Zhang et al (29) reported that low miR-138 expression levels were associated with lymph node metastasis and invasion in breast cancer. Another study revealed that in HCC, miR-138 was associated with an advanced clinical stage, the presence of portal vein invasion and lymph node metastasis (30).…”

Section: Discussionmentioning

confidence: 99%

“…miR-138/SP1-based targeted therapy could therefore represent a promising therapeutic strategy for HCC patients. Downregulation of miR-138 has been reported in diverse types of human cancer, including anaplastic thyroid carcinoma (21), head and neck squamous cell carcinoma (22), clear cell renal cell carcinoma (23), ovarian cancer (24), glioblastoma (25), colorectal cancer (26), non-small cell lung cancer (NSCLC) (27), pancreatic cancer (28) and breast cancer (29). Furthermore, reduced miR-138 expression was associated with a number of cancer characteristics.…”

Section: Discussionmentioning

confidence: 99%

“…Zhang et al (29) reported that miR-138 regulated metastasis and the epithelial-mesenchymal transition in breast cancer cells. In NSCLC, miR-138 suppressed cell proliferation, migration, in vitro cisplatin sensitivity and in vivo tumor growth (31)(32)(33).…”

Section: Discussionmentioning

confidence: 99%

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MicroRNA‑138 targets SP1 to inhibit the proliferation, migration and invasion of hepatocellular carcinoma cells

et al. 2017

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Abstract. The identification of microRNAs (miRNAs/miRs) has enabled the improved understanding of the carcinogenesis and progression of hepatocellular carcinoma (HCC). miRNAs are small non-coding RNAs comprised of 19-24 nucleotides that regulate the expression of target genes. In the present study, miR-138 was demonstrated to be downregulated in human HCC tissues and cell lines. Restoration of miR-138 expression repressed the proliferation, migration and invasion of HCC cells. Furthermore, specificity protein 1 (SP1) was identified as a target gene of miR-138 in HCC using bioinformatics analysis, luciferase reporter assay, reverse transcription-quantitative polymerase chain reaction and western blot analysis. Knockdown of SP1 produced similar suppressive effects to those induced by miR-138 overexpression in HCC cells. These results indicate that miR-138 targeted SP1 to repress the growth, migration and invasion of HCC cells, and may therefore represent a therapeutic target in human HCC.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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MicroRNA‑138 targets SP1 to inhibit the proliferation, migration and invasion of hepatocellular carcinoma cells

et al. 2017

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“…Despite various treatment methods, recurrence and metastasis are major obstacles for BC treatments (3). Studies have shown that about 40% of BC patients have experienced recurrence, and about 60-70% of patients display distant metastasis (4).…”

Section: Introductionmentioning

confidence: 99%

“…If there are no blood vessels that can provide adequate nutrients for tumor cells, the tumor volume cannot exceed 2-3 mm 3 , and organ structures and functions will also be adversely affected (10,11). As endothelial cells are necessary to form blood vessels, no clinically detectable tumors and subsidiary blood vessels will form in the absence of endothelial cell proliferation (12).…”

Section: Introductionmentioning

confidence: 99%

Involvement of breast cancer stem cells in tumor angiogenesis

Wang

et al. 2017

Oncol Lett

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Abstract. The aim of the present study was to investigate the role of breast cancer stem cells (BCSCs) in the angiogenesis of breast cancer tumors. The expression levels of mutant p53, cluster of differentiation (CD)31, vascular endothelial factor (VEGF), in addition to human epidermal growth factor (HER)2, were detected in the blood vessels of human breast cancer (BC) tissue samples. CD44 + /CD24 -/low cells were selected from single-cell suspensions of BC tissues to assess the expression of CD31 and CD105, in addition to the ability of these cells to metabolize acetylated low-density lipoprotein (Ac-LDL). Furthermore, vascular-like structures were observed histologically. Mutant p53, CD31 and VEGF were all expressed in these tissues. CD44+ cells comprised 7.5±2.6 and 94.3±4.7% of the cell population prior to and following sorting, respectively. CD24 + cells comprised 48.2±9.4 and 4.3±4% of the cell population prior to and following sorting, respectively. A low proportion of CD24 + cells corresponded to a high proportion of CD24 -/low cells. The percentages of CD105 + and CD31 + glomus cells in the mammary gland were 4.5±0.9 and 6.2±1.3%, respectively, and following passaging for three generations, these increased to 79.6±9.3 and 84.1±10.7%, respectively (P<0.05). Cells were cultured using an endothelial cell culture system, and they internalized DiL-Ac-LDL. Here, vascular endothelial cells formed vascular-like structures, whereas the control group demonstrated no such structures. Overall, the results suggest that BCSCs-derived endothelial cells may contribute to tumor angiogenesis.

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microRNA‐193a‐5p inhibits migration of human HT‐29 colon cancer cells via suppression of metastasis pathway

Shirafkan

Shomali

Kazemi

et al. 2018

J of Cellular Biochemistry

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Purpose Altered expression of microRNAs (miRNAs) is indicated strongly in colorectal cancer (CRC). This study aims to evaluate the inhibitory role of miR‐193a‐5p on epithelial‐mesenchymal transition markers in CRC lines. The cellular effects and potential mechanisms of miR‐193a‐5p were also examined. Methods Quantitative reverse‐transcription polymerase chain reaction (RT‐PCR) was performed to determine the expression of miR‐193a‐5p in three CRC cell lines (HCT‐116, SW‐480, and HT‐29) and its impact on metastasis‐related genes ( vimentin and CXCR4) before and after mimic transfection. Of those, the cell line with the highest changes was selected for the next upcoming experiments such as wound‐healing assay, 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT), and annexin‐V staining tests. Results Our results revealed that miR‐193a‐5p was significantly downregulated in three CRC cell lines and that HT‐29 displayed the most decrease ( P < 0.0001). The restoration of miR‐193a‐5p in human HT‐29 cell line inhibited cell migration. But, miR‐193a‐5p transfection did not affect cell viability and had no significant effect on apoptosis induction. Also, the quantitative RT‐PCR analysis of miR‐193a‐5p mimic transfected cells revealed a significant increase in miR‐193a‐5p messenger RNA (mRNA) expression level ( P < 0.0001) with reduction of vimentin and CXCR4 mRNA expression levels in HT‐29 cell line ( P < 0.01 and < 0.05, respectively). Conclusion Our results indicated that miR‐193a‐5p acts as a tumor suppressor miRNA and its downregulation plays an important role in metastasis via upregulation of metastasis‐related genes in CRC. Therefore, it can be considered as a potential therapeutic target for applying in CRC management in the future.

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RETRACTED: MicroRNA-138 modulates metastasis and EMT in breast cancer cells by targeting vimentin

Cited by 99 publications

References 20 publications

MicroRNA‑138 targets SP1 to inhibit the proliferation, migration and invasion of hepatocellular carcinoma cells

MicroRNA‑138 targets SP1 to inhibit the proliferation, migration and invasion of hepatocellular carcinoma cells

Involvement of breast cancer stem cells in tumor angiogenesis

microRNA‐193a‐5p inhibits migration of human HT‐29 colon cancer cells via suppression of metastasis pathway

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