RETRACTED: MicroRNA-527 Induces Proliferation and Cell Cycle in Esophageal Squamous Cell Carcinoma Cells by Repressing PH Domain Leucine-Rich-Repeats Protein Phosphatase 2

Abstract

Increasing evidence indicated that microRNAs served dominant roles in carcinogenesis and cancer progression by targeting potential downstream genes. In our study, we found that miR-527 was an upregulated expression in human esophageal squamous cell carcinoma (ESCC) cells and tissues. Furthermore, overexpression of miR-527 promoted cell proliferation and colony formation, enhanced anchorage-independent growth ability, and contributed to cell cycle. In addition, protein phosphatase 2 (PHLPP2) was identified as the direct downstream target gene of miR-527 and was confirmed by luciferase gene reporter assay. In summary, we concluded that miR-527 acted as an oncogenic microRNA in ESCC development by directly targeting PHLPP2 might be a novel therapeutic target for the treatment of ESCC.

The Role of Noncoding RNAs in the Regulation of Anoikis and Anchorage-Independent Growth in Cancer

“…The expression of miR-527 and miR-760 is upregulated in esophageal and ovarian cancer tissues, respectively, compared to their adjacent normal tissues [ 51 , 54 ]. Functional experiments showed that the anchorage-independent growth ability of cancer cells is enhanced by overexpressing either miR-527 or miR-760, in company with an increase in CCND1 levels ( Figure 1 and Table 1 ).…”

“…Functional experiments showed that the anchorage-independent growth ability of cancer cells is enhanced by overexpressing either miR-527 or miR-760, in company with an increase in CCND1 levels ( Figure 1 and Table 1 ). Besides, it was identified that both miRNAs target PH domain leucine-rich repeat-containing protein phosphatase 2 (PHLPP2), a negative regulator of cell cycle progression [ 51 , 54 ].…”

MicroRNAs, Parkinson’s Disease, and Diabetes Mellitus

“…miRNAs are synthesized from primary miRNAs in two stages by the action of two RNase III-type proteins [ 23 , 24 ]. miRNAs may regulate up to 30% of the protein-coding genes in the human genome [ 25 ] and are well known to be involved in the initiation and progression of cancers [ 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 ]. In addition to being tumor suppressors or oncogenes of cancer, miRNAs also contribute to neurological diseases.…”

MicroRNAs and Apoptosis in Colorectal Cancer

“…Their study focused on B-cell chronic lymphocytic leukemia (B-CLL) [ 40 ]. After that, many studies showed that miRNAs can initiate carcinogenesis or drive the progression of cancer [ 41 , 42 , 43 , 44 ]. The miR-527 acts as an oncogenic miRNA in esophageal squamous cell carcinoma by directly targeting PHLPP2 [ 42 ].…”

“…After that, many studies showed that miRNAs can initiate carcinogenesis or drive the progression of cancer [ 41 , 42 , 43 , 44 ]. The miR-527 acts as an oncogenic miRNA in esophageal squamous cell carcinoma by directly targeting PHLPP2 [ 42 ]. LINC02381 acts as an oncogene in the development of cervical cancer that promotes cell viability and metastasis via miR-133b [ 43 ].…”