RETRACTED: Physcion blocks cell cycle and induces apoptosis in human B cell precursor acute lymphoblastic leukemia cells by downregulating HOXA5

Gao, Fei; Liu, Wenjun; Guo, Qulian; Bai, Yiping; Yang, Hong; Chen, Hongying

doi:10.1016/j.biopha.2017.07.149

Cited by 29 publications

(22 citation statements)

References 38 publications

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“…Consistent with our results, Xudong Peng et al observed that HOXA5 inhibited GC cell proliferation in vivo [36]. In addition, another study also found that HOXA5 abrogated cell apoptosis induced by physcion, which is in line with our study [37].…”

Section: Discussionsupporting

confidence: 93%

Silencing long non-coding RNA HOTAIR exerts anti-oncogenic effect on human acute myeloid leukemia via demethylation of HOXA5 by inhibiting Dnmt3b

Wang

Huang

et al. 2019

Cancer Cell Int

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Background As an aggressive hematological malignancy, acute myeloid leukemia (AML) remains a dismal disease with poor prognosis. Long non-coding RNAs (lncRNAs) have been widely reported to be involved in tumorigenesis of AML. Here, we define an important role of lncRNA HOTAIR in AML in relation to HOXA5 methylation. Methods Firstly, the expression of HOTAIR was examined in AML samples and cells collected. Next, gain- or loss-of function experiments were conducted in AML cells to explore the effect of HOTAIR on AML. Then, relationship among HOXA5 promoter methylation, HOTAIR and Dnmt3b was measured. Expression of HOXA5 and cell proliferation/apoptosis-related genes was also detected. A last, in vivo assay was performed to assess the tumor formation in nude mice in order to explore the roles of HOTAIR and HOXA5 in cell apoptosis and proliferation. Results LncRNA HOTAIR was found to be upregulated in AML cells and tissues. With silencing of HOTAIR and overexpression of HOXA5, AML cell proliferation was decreased while the apoptosis was induced. Furthermore, HOTAIR was observed to recruit Dnmt3b and to increase HOXA5 promoter methylation. Moreover, silencing HOTAIR and upregulating HOXA5 were found to induce apoptosis and reduce proliferation of AML cells in vivo. Conclusion Our findings highlight the anti-tumor ability of HOTAIR silencing in AML, suggesting that silencing HOTAIR was able to inhibit AML progression through HOXA5 promoter demethylation by decreasing Dnmt3b. Electronic supplementary material The online version of this article (10.1186/s12935-019-0808-z) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 93%

Silencing long non-coding RNA HOTAIR exerts anti-oncogenic effect on human acute myeloid leukemia via demethylation of HOXA5 by inhibiting Dnmt3b

Wang

Huang

et al. 2019

Cancer Cell Int

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“…The anthraquinone emodin and its derivative, physcion, are major quinones in P. cuspidatum and have potential antimicrobe and anticancer applications (Jayasuriya et al, 1992; Teich et al, 2004; Su et al, 2005; Olsen et al, 2007; Yang et al, 2007; Shan et al, 2008; Narender et al, 2013; Chen et al, 2015; Xiong et al, 2015; Han et al, 2016; Hong et al, 2016; Li et al, 2016; Liu et al, 2016; Pan et al, 2016; Gao et al, 2017). Physcion significantly inhibits cancer cell proliferation and tumor growth in mice by specifically decreasing the activity of 6-phosphogluconate dehydrogenase, thus affecting the oxidative pentose phosphate pathway (Lin et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Assembly and Annotation of a Draft Genome of the Medicinal Plant Polygonum cuspidatum

Zhang

Zheng

et al. 2019

Front. Plant Sci.

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Polygonum cuspidatum (Japanese knotweed, also known as Huzhang in Chinese), a plant that produces bioactive components such as stilbenes and quinones, has long been recognized as important in traditional Chinese herbal medicine. To better understand the biological features of this plant and to gain genetic insight into the biosynthesis of its natural products, we assembled a draft genome of P. cuspidatum using Illumina sequencing technology. The draft genome is ca. 2.56 Gb long, with 71.54% of the genome annotated as transposable elements. Integrated gene prediction suggested that the P. cuspidatum genome encodes 55,075 functional genes, including 6,776 gene families that are conserved in the five eudicot species examined and 2,386 that are unique to P. cuspidatum. Among the functional genes identified, 4,753 are predicted to encode transcription factors. We traced the gene duplication history of P. cuspidatum and determined that it has undergone two whole-genome duplication events about 65 and 6.6 million years ago. Roots are considered the primary medicinal tissue, and transcriptome analysis identified 2,173 genes that were expressed at higher levels in roots compared to aboveground tissues. Detailed phylogenetic analysis demonstrated expansion of the gene family encoding stilbene synthase and chalcone synthase enzymes in the phenylpropanoid metabolic pathway, which is associated with the biosynthesis of resveratrol, a pharmacologically important stilbene. Analysis of the draft genome identified 7 abscisic acid and water deficit stress-induced protein-coding genes and 14 cysteine-rich transmembrane module genes predicted to be involved in stress responses. The draft de novo genome assembly produced in this study represents a valuable resource for the molecular characterization of medicinal compounds in P. cuspidatum, the improvement of this important medicinal plant, and the exploration of its abiotic stress resistance.

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“…The expression and dysfunction of homeobox (HOX) genes play an important role in the development of various malignant tumors, such as lung cancer, breast cancer, colon cancer, prostate cancer and leukemia (6)(7)(8)(9). HOXA5 is a member of HOX gene family and is a transcriptional regulator that regulates cell growth, differentiation and apoptosis (10,11). As the HOX genes have become a 'hot topic' in cancer research, more researchers have paid close attention concerning the relationship between HOXA5 and multiple malignancies in recent years.…”

Section: Introductionmentioning

confidence: 99%

HOXA5 inhibits the proliferation and induces the apoptosis of cervical cancer cells via regulation of protein kinase B and p27

Wang

2018

Oncol Rep

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Homeobox A5 (HOXA5) is a member of the homeobox gene (HOX) family, which plays an important role in the development of various malignant tumors. Here, we speculated that HOXA5 has an effect on cervical cancer development. In our study, we aimed to explore the role and molecular mechanism of HOXA5 in regards to the cell proliferation and apoptosis in cervical cancer. We found that expression levels of HOXA5 measured by RT-qPCR and western blot assays in cervical cancer cell lines and tissues were both significantly downregulated. We performed a gain-of-function experiment by the transfection with pcDNA.3.1-HOXA5 in ME-180 and HT-3 cells to overexpress HOXA5, and the caspase-3 activity measured by caspase-3 activity assay kit and cell apoptosis detected by flow cytometry were obviously promoted. Meanwhile, cell proliferation tested by BrdU assay, invasion determined by Transwell and cell viability tested by MTT were inhibited. Moreover, protein kinase B (AKT) was activated by incubation with SC79 (AKT activator; 1 µg/ml) after HOXA5 overexpression, and reversed the effect of HOXA5 overexpression on p27 expression. Additionally, significant elevation of AKT activation measured by western blot analysis abrogated the effect of HOXA5 on caspase-3 activity, cell apoptosis, proliferation, invasion and cell viability. Taken together, this study revealed that HOXA5 inhibits cervical cancer progression by regulating AKT/p27, proposing the potential role of HOXA5 in the prevention and treatment of cervical cancer.

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RETRACTED: Physcion blocks cell cycle and induces apoptosis in human B cell precursor acute lymphoblastic leukemia cells by downregulating HOXA5

Cited by 29 publications

References 38 publications

Silencing long non-coding RNA HOTAIR exerts anti-oncogenic effect on human acute myeloid leukemia via demethylation of HOXA5 by inhibiting Dnmt3b

Silencing long non-coding RNA HOTAIR exerts anti-oncogenic effect on human acute myeloid leukemia via demethylation of HOXA5 by inhibiting Dnmt3b

Assembly and Annotation of a Draft Genome of the Medicinal Plant Polygonum cuspidatum

HOXA5 inhibits the proliferation and induces the apoptosis of cervical cancer cells via regulation of protein kinase B and p27

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