The aim of the study was to assess the effect of natural phytoremediation, impact of growing fast-growing trees on contaminated agricultural land on ecosystem services using a Matrix evaluation system. The Matrix system is a table that links an ecosystem to its potential to provide ecosystem services. To determine the monetary price of natural capital for the provision of ecosystem services, we chose the value transfer method, based on which it is possible to assign a price (1 point = 40.7 EUR) to point values. Monitoring of the study site Kuchyňa (Mollic Fluvisol) is running since year 2010. The fastgrowing willow was planted (between 2006 to 2018 year) on an area of about 43 hectares. During the monitoring of soil parameters, we observed a slight decrease in the value of the active soil reaction, a negative trend in the content of available phosphorus and a positive trend in the development of the total content risk elements in the soil. The phytoremediation ability of willow regarding risk elements was manifested by an increase in the value and price of production and regulatory ecosystem services at the monitored location. The price of natural phytoremediation of fast-growing willow on the given area was 218762.5 EUR for production services and 171509.8 EUR for regulatory services.