RETRACTED: Potential role of andrographolide in the proliferation of osteoblasts mediated by the ERK signaling pathway

Li, Bo; Hu, Ruyin; Lee, Sun; Luo, Rui; Lu, Kai-Hang; Tian, Xiaobin

doi:10.1016/j.biopha.2016.07.033

Cited by 10 publications

(9 citation statements)

References 25 publications

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“…In addition, the mRNA and protein expression of these factors were dramatically decreased after 20 μmol L −1 XAV‐939 treatment, indicating that XAV‐939 can significantly reversed GP‐12‐induced upregulation in mRNA and protein expression of the above genes ( P < 0.05). A number of previous studies have demonstrated similar results during the investigation of osteoblasts proliferation . Taken together, we proposed that GP‐12 was probably exerting its functions by upregulating the expression of Cyclin D1 and Runx 2; and it then activated the downstream transcription factor (Osterix) and promoted the synthesis and secretion of Col I, and hence promoting the osteoblastic proliferation and differentiation.…”

Section: Resultssupporting

confidence: 75%

“…As expected, the mRNA and protein expression of these factors were dramatically downregulated after 20 μmol L −1 XAV‐939 treatment, indicating that XAV‐939 can significantly reversed GP‐12‐induced upregulations in mRNA and protein expression of the above genes ( P < 0.05), which further confirmed that Wnt/ β ‐catenin pathway plays a crucial role in GP‐12‐induced osteoblasts proliferation and differentiation. A number of previous studies have demonstrated similar results during the investigation of osteoblasts proliferation …”

Section: Resultsmentioning

confidence: 99%

“…A number of previous studies have demonstrated similar results during the investigation of osteoblasts proliferation. 20,37,38 Taken together, we proposed that GP-12 was probably exerting its functions by upregulating the expression of Cyclin D1 and Runx 2; and it then activated the downstream transcription factor (Osterix) and promoted the synthesis and secretion of Col I, and hence promoting the osteoblastic proliferation and differentiation.…”

Section: Wileyonlinelibrarycom/jsfamentioning

confidence: 87%

“…20,38 As expected, the mRNA and protein expression of these factors were dramatically downregulated after 20 μmol L −1 XAV-939 treatment, indicating that XAV-939 can significantly reversed GP-12-induced upregulations in mRNA and protein expression of the above genes (P < 0.05), which further confirmed that Wnt/⊎-catenin pathway plays a crucial role in GP-12-induced osteoblasts proliferation and differentiation. A 15,20,37 An increased mRNA and protein expression of GSK-3⊎ were observed after treatment with GP-12. It is recognized that the GSK-3⊎, which phosphorylates and promotes the degradation of ⊎-catenin in quiescent cells, is a key negative regulator of ⊎-catenin in the canonical Wnt/⊎-catenin signaling pathway.…”

Section: Wileyonlinelibrarycom/jsfamentioning

confidence: 99%

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Yak (Bos grunniens) bones collagen‐derived peptides stimulate osteoblastic proliferation and differentiation via the activation of Wnt/β‐catenin signaling pathway

Zhang

Zhu

et al. 2020

J Sci Food Agric

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BACKGROUND As the world's population is transitioning gradually to an ageing stage, the incidence of osteoporosis is increasing annually. Yak bone is one of the major components of Tibetan medicine and it has mainly been associated with an improvement in bone health, for example against osteoporosis. However, the functional bioactive ingredients and the underlying mechanisms are still unclear. RESULTS Sequential purification of yak‐bone hydrolysates was achieved by ultrafiltration, size exclusion chromatography, and semi‐preparative reverse‐phase high‐performance liquid chromatography. After this, 35 novel peptides were identified by mass spectrometry analysis, of which peptide GPAGPPGPIGNV (GP‐12) displayed the highest osteoblast proliferation‐promoting activity, with an increase of 42.7% in cell growth. An in vitro stability study demonstrated that GP‐12 was digested into smaller peptides (GP‐9, GV‐9, AV‐10 and GP‐11) after simulated gastrointestinal digestion and absorption (Caco‐2 cell monolayers) experiments. However, some of them still can be absorbed intact through the (Caco‐2 cell monolayers by a paracellular route (Papp: 5.36 ± 0.34 cm s−1). Flow cytometry results indicated that GP‐12 enhanced osteoblastic proliferation by inducing the alteration of the cell‐cycle progression both from the G0/G1 to the S phase and from the S to the G2/M phase. Quantitative real‐time polymerase chain reaction (PCR) and western blot results revealed that GP‐12 induced osteoblastic proliferation and differentiation in a dose–response manner through the activation of a Wnt/β‐catenin signaling pathway. CONCLUSION These findings highlighted that such peptides hold the promise of discovering candidates for functional and health‐promoting foods, which could be potentially used for the treatment of osteoporosis. © 2020 Society of Chemical Industry

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Section: Resultssupporting

confidence: 75%

Section: Resultsmentioning

confidence: 99%

Section: Wileyonlinelibrarycom/jsfamentioning

confidence: 87%

Section: Wileyonlinelibrarycom/jsfamentioning

confidence: 99%

See 2 more Smart Citations

Yak (Bos grunniens) bones collagen‐derived peptides stimulate osteoblastic proliferation and differentiation via the activation of Wnt/β‐catenin signaling pathway

Zhang

Zhu

et al. 2020

J Sci Food Agric

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“…Parthenolide, AP and other terpenoids exerting an effect on osteoclasts or osteoblasts has been found in vitro (Kim et al, 2014;Qu et al, 2014;Zhang et al, 2014;Li et al, 2016). But whether they have any effect on bone destruction in RA is unknown, it is critical to verified terpenoids' pharmacological action of bone protection in vivo.…”

Section: Terpenoidsmentioning

confidence: 99%

Potential Advantages of Bioactive Compounds Extracted From Traditional Chinese Medicine to Inhibit Bone Destructions in Rheumatoid Arthritis

et al. 2020

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Bone destruction is an important pathological feature of rheumatoid arthritis (RA), which finally leads to the serious decline of life quality in RA patients. Bone metabolism imbalance is the principal factor of bone destruction in RA, which is manifested by excessive osteoclast-mediated bone resorption and inadequate osteoblast-mediated bone formation. Although current drugs alleviate the process of bone destruction to a certain extent, there are still many deficiencies. Recent studies have shown that traditional Chinese medicine (TCM) could effectively suppress bone destruction of RA. Some bioactive compounds from TCM have shown good effect on inhibiting osteoclast differentiation and promoting osteoblast proliferation. This article reviews the research progress of bioactive compounds exacted from TCM in inhibiting bone destruction of RA, so as to provide references for further clinical and scientific research.

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An overview on the potential biomedical applications of polysaccharides

Rajalekshmy

Devi

Joseph

et al. 2019

Functional Polysaccharides for Biomedical Applications

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RETRACTED: Potential role of andrographolide in the proliferation of osteoblasts mediated by the ERK signaling pathway

Cited by 10 publications

References 25 publications

Yak (Bos grunniens) bones collagen‐derived peptides stimulate osteoblastic proliferation and differentiation via the activation of Wnt/β‐catenin signaling pathway

Yak (Bos grunniens) bones collagen‐derived peptides stimulate osteoblastic proliferation and differentiation via the activation of Wnt/β‐catenin signaling pathway

Potential Advantages of Bioactive Compounds Extracted From Traditional Chinese Medicine to Inhibit Bone Destructions in Rheumatoid Arthritis

An overview on the potential biomedical applications of polysaccharides

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