RETRACTED: The effect of DNA-PKcs gene silencing on proliferation, migration, invasion and apoptosis, and in vivo tumorigenicity of human osteosarcoma MG-63 cells

Jin, Peiying; Lu, Hong-Jie; Tang, Yao; Fan, Shao‐Hua; Zhang, Zi-Feng; Wang, Yan; Li, Xu-Ning; Wu, Dongmei; Lü, Jun; Zheng, Yuxin

doi:10.1016/j.biopha.2017.11.079

Cited by 10 publications

(4 citation statements)

References 40 publications

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“…Cell migration was determined using Transwell chambers as previously described [16]. Briefly, cells were starved overnight, and suspended with the concentration of 1 × 10 5 per well.…”

Section: Cell Migrationmentioning

confidence: 99%

lncRNA MIAT suppression alleviates corneal angiogenesis through regulating miR-1246/ACE

et al. 2019

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Corneal neovascularization (CRNV) is a prevalence eye disorder that affects the transparency and refraction properties of eyes. To explore the correlation between the level of Angiotensin II (Ang II) and corneal angiogenesis, the rat model of CRNV was established using alkali-burn, while the human umbilical vein endothelial cells (HUVECs) were stimulated using VEGF to induce the CRNV cells in vitro. RNA immunoprecipitation (RIP) and RNA pull-down were performed to validate the relationship between MIAT and miR-1246. The expression of MIAT and Ang II was increased, while miR-1246 was decreased in CRNV rat model. VEGF stimulation significantly promoted cell proliferation and migration of HUVECs, knockdown of MIAT dramatically reversed the effects of VEGF, while cells co-transfected with miR-1246 inhibitor obviously abolished the effect of VEGF+si-MIAT, however, enalaprilat abolished the effects of VEGF+si-MIAT+miR-1246 inhibitor. MIAT directly regulated the expression of miR-1246. In conclusion, VEGF stimulation promoted cell proliferation and migration of HUVECs mainly through regulating MIAT/miR-1246/ACE. ARTICLE HISTORY

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“…Cell migration was determined using Transwell chambers as previously described [16]. Briefly, cells were starved overnight, and suspended with the concentration of 1 × 10 5 per well.…”

Section: Cell Migrationmentioning

confidence: 99%

lncRNA MIAT suppression alleviates corneal angiogenesis through regulating miR-1246/ACE

et al. 2019

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“…Upon moving into the nucleus, EGFR can act as a transcription factor for a series of oncogenes to accelerate cellular proliferation and reduce apoptosis (39)(40)(41). Besides, silencing of DNA-PK is able to promote cell apoptosis (42). As the link between EGFR signaling pathways and DNA repair mechanisms has been demonstrated (43), we considered that EGFR transports into cell nucleus after irradiation, and triggers DNA repair through association with DNA-PK, which then decreases cell apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Ginsenoside Rg3 Sensitizes Nasopharyngeal Carcinoma Cells to Radiation by Suppressing Epithelial Mesenchymal Transition

Luo

Guo

et al. 2023

Radiation Research

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Radioresistance restrains the therapeutic effect of nasopharyngeal carcinoma (NPC). Ginsenoside Rg3 (Rg3), an active pharmaceutical component extracted from ginseng, shows antitumor effects in various cancers. In this study, we aimed to determine whether Rg3 sensitized NPC cells to radiation and to explore the possible mechanisms. Our results revealed that Rg3 increased radiosensitivity in both HNE1 and CNE2 cell lines. Radiation-induced epithelial mesenchymal transition (EMT) in NPC cells and Rg3 blocked this effect. In addition, Rg3 attenuated radiation-induced epidermal growth factor receptor (EGFR) nuclear transport and DNA-dependent protein kinase expression. What's more, Rg3 significantly accelerated the apoptosis rates in irradiated NPC cells. In summary, our data suggested that Rg3 sensitized NPC cells to radiation and suppressed radiation-induced EMT. This effect is mediated through restrained EGFR nuclear translocation and increased cell apoptosis. Thus, Rg3 may be a potential radiation sensitizing agent for NPC.

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“…For this study, we adopted a subcutaneous osteosarcoma mouse model. 26,27 Although an in situ mouse experimental proximal bone model has also been broadly used for osteosarcoma research, [28][29][30][31] the subcutaneous tumor model has the advantage of establishing the direct observation of the tumor growth and being able to readily collect important data such as animal weight, tumor growth curve, and tumor weight. At the same time, a subcutaneous tumor can be used for correlation analysis of tissue and body fluid samples, which is widely used in biomedical research and drug development.…”

Section: Discussionmentioning

confidence: 99%

DEPDC1as a crucial factor in the progression of human osteosarcoma

Shen

Liu

et al. 2022

Cancer Medicine

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Objective: Novel therapeutic strategies are emerging with the increased understanding of the underlying mechanisms of human osteosarcoma. This current study tends to decipher the potentially critical role of DEP domain-containing 1 (DEPDC1), a tumor-related gene, during the progression of osteosarcoma.Methods: Bioinformatics analysis of 25,035 genes from the National Center for Biotechnology Information (NCBI) databases was performed to screen differentially expressed genes between osteosarcoma and normal control groups, complemented by the examination of 85 clinical osteosarcoma specimens. Furthermore, the manipulation of DEPDC1 expression levels by using silencing RNA (siRNA) or lentiviral vector intervention on human osteosarcoma cells was performed to reveal its role and interactions in in vitro and in vivo settings. Results: Gene expression profile analysis and immunohistochemical (IHC) examination suggested that DEPDC1 is highly expressed in human osteosarcoma cells and tumor tissue. The silencing of DEPDC1 arrested osteosarcoma cell proliferation, promoted apoptosis, and ceased tumor metastasis. Studies involving clinical human osteosarcoma cases exhibited a strong correlation of DEPDC1 over-expressed osteosarcoma specimens with a reduced patient survival rate. Conclusions: Collectively, this study demonstrated that DEPDC1 is a critical driver in the promotion of osteosarcoma progression and results in poor patient

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RETRACTED: The effect of DNA-PKcs gene silencing on proliferation, migration, invasion and apoptosis, and in vivo tumorigenicity of human osteosarcoma MG-63 cells

Cited by 10 publications

References 40 publications

lncRNA MIAT suppression alleviates corneal angiogenesis through regulating miR-1246/ACE

lncRNA MIAT suppression alleviates corneal angiogenesis through regulating miR-1246/ACE

Ginsenoside Rg3 Sensitizes Nasopharyngeal Carcinoma Cells to Radiation by Suppressing Epithelial Mesenchymal Transition

DEPDC1as a crucial factor in the progression of human osteosarcoma

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