RETRACTED: The myth of plant transformation

Rao, Abdul Qayyum; Bakhsh, Allah; Kiani, Sarfraz; Shahzad, Kamran; Shahid, Ahmad Ali; Husnain, Tayyab; Riazuddin, Sheikh

doi:10.1016/j.biotechadv.2009.04.028

Cited by 102 publications

(70 citation statements)

References 127 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…After 3 d, embryos were subcultured on MS medium containing kanamycin 50 mg/ml optimized for cotton plants for selection. After two months of selection on kanamycin medium, putative transgenic plants were shifted in shoot and root regeneration media without kanamycin, as determined by Rao et al (2009). The healthy putative transgenic cotton plants were shifted to pots containing loamy soil.…”

Section: Plant Transformationmentioning

confidence: 99%

Overexpression of the phytochrome B gene from Arabidopsis thaliana increases plant growth and yield of cotton (Gossypium hirsutum)

Rao

Irfan

Saleem

et al. 2011

J. Zhejiang Univ. Sci. B

Self Cite

View full text Add to dashboard Cite

Abstract:The phytochrome B (PHYB) gene of Arabidopsis thaliana was introduced into cotton through Agrobacterium tumefaciens. Integration and expression of PHYB gene in cotton plants were confirmed by molecular evidence. Messenger RNA (mRNA) expression in one of the transgenic lines, QCC11, was much higher than those of control and other transgenic lines. Transgenic cotton plants showed more than a two-fold increase in photosynthetic rate and more than a four-fold increase in transpiration rate and stomatal conductance. The increase in photosynthetic rate led to a 46% increase in relative growth rate and an 18% increase in net assimilation rate. Data recorded up to two generations, both in the greenhouse and in the field, revealed that overexpression of Arabidopsis thaliana PHYB gene in transgenic cotton plants resulted in an increase in the production of cotton by improving the cotton plant growth, with 35% more yield. Moreover, the presence of the Arabidopsis thaliana PHYB gene caused pleiotropic effects like semi-dwarfism, decrease in apical dominance, and increase in boll size.

show abstract

Section: Plant Transformationmentioning

confidence: 99%

Overexpression of the phytochrome B gene from Arabidopsis thaliana increases plant growth and yield of cotton (Gossypium hirsutum)

Rao

Irfan

Saleem

et al. 2011

J. Zhejiang Univ. Sci. B

Self Cite

View full text Add to dashboard Cite

show abstract

“…The ability of Agrobacterium to genetically transform a wide variety of plant and non-plant species has earned it an honour of "nature's genetic engineer" and placed it at the forefront of future biotechnological applications (Rao et al, 2009). However, the Agrobacterium-mediated genetic transformation is still an extremely inefficient process, in which only few of the host cells can be infected, and T-DNA integration and stable expression occur in an even smaller fraction of the infected cells.…”

Section: Discussionmentioning

confidence: 99%

Agrobacterium-Mediated Genetic Transformation: History and Progress

Guo¹,

Bian²,

Xiao³

et al. 2011

Genetic Transformation

View full text Add to dashboard Cite

“…After more than 25 years, Agrobacterium-mediated gene transfer is still the most used method for fruit species transformation including apple, almond, banana, orange, grapevine, melon etc. (Table 1; Rao et al, 2009). …”

Section: A Complex Relationshipmentioning

confidence: 99%

“…Plant genetic engineering has opened new avenues to modify crops, and provided new solutions to solve specific needs (Rao et al, 2009). Contrary to conventional plant breeding, this technology can integrate foreign DNA into different plant cells to produce transgenic plants with new desirable traits (Chilton et al, 1977;Newell, 2000).…”

Section: Introductionmentioning

confidence: 99%